Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1976 Sep;25(3):437–441.

In vitro monitoring of cadaver kidney donor pretreatment by lymphocyte culture.

J R Jeffery, R D Guttmann, B Charpentier
PMCID: PMC1541421  PMID: 134860

Abstract

Fourteen cadaver kidney donors were pretreated with a rapid infusion of 5 g of cyclophosphamide and 5 g of methylprednisolone in an attempt to reduce graft immunogenicity by altering immunogenic "passenger" leucocytes. The effects of this drug infusion on peripheral lymphocytes were monitored by measuring the ability of donor lymphocytes to respond and to stimulate recipient cells in mixed lymphocyte culture and by measuring their ability to respond to phytohaemagglutinin stimulation before and after infusion of the drugs. Pretreatment severely decreased the ability of donor cells to function in these tests, but the maximum effect was not seen until several hours after completion of the infusion. The results of this study suggest that donor nephrectomy should be delayed for at least 4 hr after pretreatment to obtain maximum lymphocyte effect using this regimen. This type of in vitro testing may provide means of monitoring donor pretreatment and comparing different pretreatment protocols in human cadaveric allotransplantation.

Full text

PDF
437

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Billingham R. E. The passenger cell concept in transplantation immunology. Cell Immunol. 1971 Feb;2(1):1–12. doi: 10.1016/0008-8749(71)90022-0. [DOI] [PubMed] [Google Scholar]
  2. Boylston A. W., Guttmann R. D., Merrill J. P. A simplified method for the quantitation of in vitro leucocyte culture. Int Arch Allergy Appl Immunol. 1968;34(4):339–344. doi: 10.1159/000230128. [DOI] [PubMed] [Google Scholar]
  3. Elkins W. L., Guttmann R. D. Pathogenesis of a local graft versus host reaction: immunogenicity of circulating host leukocytes. Science. 1968 Mar 15;159(3820):1250–1251. doi: 10.1126/science.159.3820.1250. [DOI] [PubMed] [Google Scholar]
  4. Etheredge E. E., Shons A. R., Hohenthaner K. L., Najarian J. S. Mitomycin C inactivation of leukocytes in the mixed leukocyte culture. Transplantation. 1973 Mar;15(3):331–334. doi: 10.1097/00007890-197303000-00012. [DOI] [PubMed] [Google Scholar]
  5. Freeman J. S., Chamberlain E. C., Reemtsma K., Steinmuller D. Prolongation of rat heart allografts by donor pretreatment with immunosuppressive agents. Transplant Proc. 1971 Mar;3(1):580–582. [PubMed] [Google Scholar]
  6. Guttman R. D., Beaudoin J. G., Morehouse D. D., Klassen J., Knaack J., Jeffery J., Chassot P. G., Abbou C. C. Donor pretreatment as an adjunct to cadaver renal allotransplantation. Transplant Proc. 1975 Mar;7(1):117–121. [PubMed] [Google Scholar]
  7. Guttmann R. D., Beaudoin J. G., Morehouse D. D. Reduction of immunogenicity of human cadaver renal allografts by donor pretreatment. Transplant Proc. 1973 Mar;5(1):663–665. [PubMed] [Google Scholar]
  8. Guttmann R. D., Carpenter C. B., Lindquist R. R., Merrill J. P. An immunosupressive site of action of heterologous antilymphocyte serum. Lancet. 1967 Feb 4;1(7484):248–249. doi: 10.1016/s0140-6736(67)91306-2. [DOI] [PubMed] [Google Scholar]
  9. Guttmann R. D., Falk R. E., Kuramochi T. Thymic influences on cardiac allograft immunogenicity. Transplantation. 1974 Aug;18(2):93–98. doi: 10.1097/00007890-197408000-00001. [DOI] [PubMed] [Google Scholar]
  10. Guttmann R. D., Lindquist R. R., Ockner S. A. Renal transplantation in the inbred rat. IX. Hematopoietic origin of an immunogenic stimulus of rejection. Transplantation. 1969 Oct;8(4):472–484. doi: 10.1097/00007890-196910000-00019. [DOI] [PubMed] [Google Scholar]
  11. Guttmann R. D., Lindquist R. R. Renal transplantation in the inbred rat. XI. Reduction of allograft immunogenicity by cytotoxic drug pretreatment of donors. Transplantation. 1969 Oct;8(4):490–495. [PubMed] [Google Scholar]
  12. Hartzman R. J., Segall M., Bach M. L., Bach F. H. Histocompatibility matching. VI. Miniaturization of the mixed leukocyte culture test: a preliminary report. Transplantation. 1971 Mar;11(3):268–273. doi: 10.1097/00007890-197103000-00005. [DOI] [PubMed] [Google Scholar]
  13. Malek G. H., Richards M. J., Burkholder P. M., Caldwell W. L. Effect of single dose irradiation on isolated perfused canine kidney. Surgery. 1974 Aug;76(2):272–277. [PubMed] [Google Scholar]
  14. Millar D., Salaman J. R. The sensitising properties of renal perfusate. Transplantation. 1974 Sep;18(3):251–255. doi: 10.1097/00007890-197409000-00008. [DOI] [PubMed] [Google Scholar]
  15. Plate J. M., McKenzie I. F. "B"-cell stimulation of allogeneic T-cell proliferation in mixed lymphocyte cultures. Nat New Biol. 1973 Oct 24;245(147):247–249. doi: 10.1038/newbio245247a0. [DOI] [PubMed] [Google Scholar]
  16. Steinmuller D., Hart E. A. Passenger leukocytes and induction of allograft immunity. Transplant Proc. 1971 Mar;3(1):673–675. [PubMed] [Google Scholar]
  17. Steinmuller D., Warden G., Coleman M., Lofgreen J., Reemtsma K., Stuart F., Garrick T., Holter A., Lynch A. Prolonged survival of rat heart and kidney allografts irradiated in vitro. Transplantation. 1971 Aug;12(2):153–156. doi: 10.1097/00007890-197108000-00012. [DOI] [PubMed] [Google Scholar]
  18. Stuart F. P., Bastien E., Holter A., Fitch F. W., Elkins W. L. Role of passenger leukocytes in the rejection of renal allografts. Transplant Proc. 1971 Mar;3(1):461–464. [PubMed] [Google Scholar]
  19. Stuart F. P., Garrick T., Holter A., Lynch A., Bastien E. Delayed rejection of renal allografts in the rat and dog by reduction of passenger leukocytes. Surgery. 1971 Jul;70(1):128–134. [PubMed] [Google Scholar]
  20. Torkelson A. R., LaBudde J. A., Weikel J. H., Jr The metabolic fate of cyclophosphamide. Drug Metab Rev. 1974;3(1):131–165. doi: 10.3109/03602537408993740. [DOI] [PubMed] [Google Scholar]
  21. Woods J. E., Cooperman A. M., Holley K. E. Attempted antigenic alteration of canine kidneys by 24- or 48-hr perfusion with immunosuppressive agents. Transplantation. 1972 Mar;13(3):336–336. doi: 10.1097/00007890-197203000-00023. [DOI] [PubMed] [Google Scholar]
  22. Zincke H., Woods J. E. Attempted immunological alteration of canine renal allograft donors. Transplantation. 1974 Dec;18(6):480–486. doi: 10.1097/00007890-197412000-00002. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES