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. 1988 Feb;71(2):362–367.

Shedding and synthesis de novo of Fc and C3b receptors by cultured guinea-pig macrophages.

G A Limb 1, K A Brown 1, R A Wolstencroft 1, D C Dumonde 1
PMCID: PMC1541439  PMID: 2964964

Abstract

Resident macrophages freshly obtained from the peritoneal cavity of guinea-pigs were demonstrated to form a higher percentage of Fc and C3b rosettes than elicited macrophages when low concentrations of IgG and IgM-C3b were used to sensitize ox red blood cells (ORBC) in rosette assays. Culture of the total resident and elicited macrophages for 6 h at 37 degrees C resulted in a decrease of Fc and C3b rosette-forming cells, the loss of Fc receptor-bearing cells by resident macrophages only being apparent when using a sub-optimal concentration of sensitizing IgG. After 24 h incubation the percentages of Fc and C3b rosettes returned to their initial values. In contrast, there was no decline in the percentage of Fc and C3b rosettes formed by the adherent population of resident and elicited macrophages cultured for 6 h. However, extending the incubation of the adherent macrophage to 24 h produced an increase of Fc receptor-positive cells and a dramatic decrease of C3b receptor-positive cells. Culture supernatants of the total macrophage population that had been incubated for 6 h inhibited Fc and C3b rosette formation by freshly obtained elicited macrophages. These results, together with the demonstration that treatment of the total macrophage population with cycloheximide led to an inhibition of Fc and C3b receptor expression after 24 h culture, suggest that the Fc and C3b receptors of guinea-pig macrophages are shed and synthesized de novo during short-term culture. This system could be applied to the study in vitro of soluble immunoregulatory mediators on macrophage functions which are dependent on the expression of Fc and C3b receptors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arend W. P., Mannik M. The macrophage receptor for IgG: number and affinity of binding sites. J Immunol. 1973 Jun;110(6):1455–1463. [PubMed] [Google Scholar]
  2. Berger M., Cross A. S. Lymphoblastoid cell supernatants increase expression of C3b receptors on human polymorphonuclear leucocytes: direct binding studies with 125I-C3b. Immunology. 1984 Mar;51(3):431–440. [PMC free article] [PubMed] [Google Scholar]
  3. Bianco C., Griffin F. M., Jr, Silverstein S. C. Studies of the macrophage complement receptor. Alteration of receptor function upon macrophage activation. J Exp Med. 1975 Jun 1;141(6):1278–1290. doi: 10.1084/jem.141.6.1278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ehlenberger A. G., Nussenzweig V. The role of membrane receptors for C3b and C3d in phagocytosis. J Exp Med. 1977 Feb 1;145(2):357–371. doi: 10.1084/jem.145.2.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Guimezanes A., Fridman W. H., Kourilsky F. M. Immunoglobulin binding factor (IBF) produced by T cells: selective interaction with slow migrating rabbit IgC. Ann Immunol (Paris) 1975 Oct-Dec;126(5-6):541–551. [PubMed] [Google Scholar]
  6. Kay N. E., Douglas S. D. Detection of shedding of human blood monocyte Fc receptor during in vitro culture. Int Arch Allergy Appl Immunol. 1981;66(2):131–135. doi: 10.1159/000232811. [DOI] [PubMed] [Google Scholar]
  7. Kurnick J. T., Ostberg L., Stegagno M., Kimura A. K., Orn A., Sjöberg O. A rapid method for the separation of functional lymphoid cell populations of human and animal origin on PVP-silica (Percoll) density gradients. Scand J Immunol. 1979;10(6):563–573. doi: 10.1111/j.1365-3083.1979.tb01391.x. [DOI] [PubMed] [Google Scholar]
  8. Lee S. T., Paraskevas F. Macrophage--T cell interactions. I. The uptake by T cells of Fc receptors released from macrophages. Cell Immunol. 1978 Sep 15;40(1):141–153. doi: 10.1016/0008-8749(78)90322-2. [DOI] [PubMed] [Google Scholar]
  9. Mantovani B., Rabinovitch M., Nussenzweig V. Phagocytosis of immune complexes by macrophages. Different roles of the macrophage receptor sites for complement (C3) and for immunoglobulin (IgG). J Exp Med. 1972 Apr 1;135(4):780–792. doi: 10.1084/jem.135.4.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Molenaar J. L., van Galen M., Hannema A. J., Zeijlemaker W., Pondman K. W. Spontaneous release of Fc receptor-like material from human lymphoblastoid cell lines. Eur J Immunol. 1977 Apr;7(4):230–236. doi: 10.1002/eji.1830070409. [DOI] [PubMed] [Google Scholar]
  11. Nathan C., Brukner L., Kaplan G., Unkeless J., Cohn Z. Role of activated macrophages in antibody-dependent lysis of tumor cells. J Exp Med. 1980 Jul 1;152(1):183–197. doi: 10.1084/jem.152.1.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Papamichail M., Tsokos G., Paraskevas F. Release and reconstitution of Fc receptors from normal human mononuclear cells. Immunology. 1980 May;40(1):47–52. [PMC free article] [PubMed] [Google Scholar]
  13. Rhodes J. Macrophage heterogeneity in receptor activity: the activation of macrophage Fc receptor function in vivo and in vitro. J Immunol. 1975 Mar;114(3):976–981. [PubMed] [Google Scholar]
  14. Scott C. S. Maturation-linked expression of C3b and C3b' receptors on developing human bone marrow and peripheral blood granulocytes. Clin Exp Immunol. 1981 Apr;44(1):201–210. [PMC free article] [PubMed] [Google Scholar]
  15. Sármay G., István L., Gergely J. Shedding and reappearance of Fc, C3 and SRBC receptors on peripheral lymphocytes from normal donors and chronic lymphatic leukaemia (CLL) patients. Immunology. 1978 Feb;34(2):315–321. [PMC free article] [PubMed] [Google Scholar]
  16. Wong L., Wilson J. D. The identification of Fc and C3 receptors on human neutrophils. J Immunol Methods. 1975 Apr;7(1):69–76. doi: 10.1016/0022-1759(75)90131-3. [DOI] [PubMed] [Google Scholar]
  17. van der Poel J. J., Bradley B. A. Modified micromethod for detection of human Fc receptor-like material. J Immunol Methods. 1977;16(2):111–117. doi: 10.1016/0022-1759(77)90046-1. [DOI] [PubMed] [Google Scholar]

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