Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1988 Jul;73(1):111–116.

Suppressor cell activity of human alveolar macrophages in interstitial lung diseases.

E M Fireman 1, S Ben Efraim 1, J Greif 1, S Kivity 1, M R Topilsky 1
PMCID: PMC1541470  PMID: 2971484

Abstract

It has been shown that alveolar macrophages (AM) are able to modulate lymphocyte proliferation in vitro. The purpose of this study was to evaluate the effect of AM on the proliferation of autologous peripheral lymphocytes (APL) in patients with interstitial lung disease (ILD) compared to controls. Thirty patients were investigated: eight with idiopathic pulmonary fibrosis (IPF), nine with sarcoidosis(SA), seven with rheumatoid arthritis (RA) and six controls (CO). AM and APL were co-cultured at an increasing macrophage/lymphocyte ratio: 1%, 10%, 20% and 50%. A dose-dependent effect was observed and related to the number of AM added to APL, enhancing at low ratios and suppressing at high ratios. Suppression of proliferation by 50% AM differed in the four groups tested: 94.6% (92-98) in IPF, 73.0% (49-100) in SA, 43% (25-57) in RA, 32.4% (22-41) in the CO (P less than 0.01, P less than 0.05, and P0.05 respectively, compared to CO). Suppressive cell activity of AM from patients with ILD was higher than suppressive cell activity of the CO group. Suppression with 10% of AM in ILD group was 18% (2.2-62) compared with 2.58% (-13-17) in the CO group (P less than 0.05). 20% AM in ILD group showed 35% (3-76) suppression in comparison with 9.76% (-11-27) in the control group (P less than 0.01), 50% AM in ILD have a suppressive activity of 71% (25-100) in contrast to 32.4% (22-41) in control (P less than 0.01). In conclusion, AM from patients with interstitial lung diseases have a significantly stronger suppressive effect on the proliferation of autologous peripheral lymphocytes than controls. This is a new aspect of the study of activation of AM in these kinds of disorders.

Full text

PDF
111

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachwich P. R., Lynch J. P., 3rd, Kunkel S. L. Arachidonic acid metabolism is altered in sarcoid alveolar macrophages. Clin Immunol Immunopathol. 1987 Jan;42(1):27–37. doi: 10.1016/0090-1229(87)90170-x. [DOI] [PubMed] [Google Scholar]
  2. Bitterman P. B., Rennard S. I., Hunninghake G. W., Crystal R. G. Human alveolar macrophage growth factor for fibroblasts. Regulation and partial characterization. J Clin Invest. 1982 Oct;70(4):806–822. doi: 10.1172/JCI110677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark J. G., Kostal K. M., Marino B. A. Bleomycin-induced pulmonary fibrosis in hamsters. An alveolar macrophage product increases fibroblast prostaglandin E2 and cyclic adenosine monophosphate and suppresses fibroblast proliferation and collagen production. J Clin Invest. 1983 Dec;72(6):2082–2091. doi: 10.1172/JCI111173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crystal R. G., Bitterman P. B., Rennard S. I., Hance A. J., Keogh B. A. Interstitial lung diseases of unknown cause. Disorders characterized by chronic inflammation of the lower respiratory tract (first of two parts). N Engl J Med. 1984 Jan 19;310(3):154–166. doi: 10.1056/NEJM198401193100304. [DOI] [PubMed] [Google Scholar]
  5. DeLustro F., Sherer G. K., LeRoy E. C. Human monocyte stimulation of fibroblast growth by a soluble mediator(s). J Reticuloendothel Soc. 1980 Dec;28(6):519–532. [PubMed] [Google Scholar]
  6. Elias J. A., Zurier R. B., Rossman M. D., Berube M. L., Daniele R. P. Inhibition of lung fibroblast growth by human lung mononuclear cells. Am Rev Respir Dis. 1984 Nov;130(5):810–816. doi: 10.1164/arrd.1984.130.5.810. [DOI] [PubMed] [Google Scholar]
  7. Ettensohn D. B., Roberts N. J., Jr Human alveolar macrophage support of lymphocyte responses to mitogens and antigens. Analysis and comparison with autologous peripheral-blood-derived monocytes and macrophages. Am Rev Respir Dis. 1983 Sep;128(3):516–522. doi: 10.1164/arrd.1983.128.3.516. [DOI] [PubMed] [Google Scholar]
  8. Fujiwara H., Ellner J. J. Spontaneous production of a suppressor factor by the human macrophage-like cell line U937. I. Suppression of interleukin 1, interleukin 2, and mitogen-induced blastogenesis in mouse thymocytes. J Immunol. 1986 Jan;136(1):181–185. [PubMed] [Google Scholar]
  9. Holt P. G. Alveolar macrophages. II. Inhibition of lymphocyte proliferation by purified macrophages from rat lung. Immunology. 1979 Jun;37(2):429–436. [PMC free article] [PubMed] [Google Scholar]
  10. Holt P. G. Alveolar macrophages. III. Studies on the mechanisms of inhibition of T-cell proliferation. Immunology. 1979 Jun;37(2):437–445. [PMC free article] [PubMed] [Google Scholar]
  11. Hunninghake G. W., Gadek J. E., Kawanami O., Ferrans V. J., Crystal R. G. Inflammatory and immune processes in the human lung in health and disease: evaluation by bronchoalveolar lavage. Am J Pathol. 1979 Oct;97(1):149–206. [PMC free article] [PubMed] [Google Scholar]
  12. Hunninghake G. W., Kawanami O., Ferrans V. J., Young R. C., Jr, Roberts W. C., Crystal R. G. Characterization of the inflammatory and immune effector cells in the lung parenchyma of patients with interstitial lung disease. Am Rev Respir Dis. 1981 Apr;123(4 Pt 1):407–412. doi: 10.1164/arrd.1981.123.4.407. [DOI] [PubMed] [Google Scholar]
  13. Hunninghake G. W. Release of interleukin-1 by alveolar macrophages of patients with active pulmonary sarcoidosis. Am Rev Respir Dis. 1984 Apr;129(4):569–572. [PubMed] [Google Scholar]
  14. Lawrence E. C., Theodore B. J., Martin R. R. Modulation of pokeweed-mitogen-induced immunoglobulin secretion by human bronchoalveolar cells. Am Rev Respir Dis. 1982 Aug;126(2):248–252. doi: 10.1164/arrd.1982.126.2.248. [DOI] [PubMed] [Google Scholar]
  15. Lee J. C., Gibson C. W., Eisenstein T. K. Macrophage-mediated mitogenic suppression induced in mice of the C3H lineage by a vaccine strain of Salmonella typhimurium. Cell Immunol. 1985 Mar;91(1):75–91. doi: 10.1016/0008-8749(85)90033-4. [DOI] [PubMed] [Google Scholar]
  16. Liu M. C., Proud D., Schleimer R. P., Plaut M. Human lung macrophages enhance and inhibit lymphocyte proliferation. J Immunol. 1984 Jun;132(6):2895–2903. [PubMed] [Google Scholar]
  17. McCombs C. C., Michalski J. P., Westerfield B. T., Light R. W. Human alveolar macrophages suppress the proliferative response of peripheral blood lymphocytes. Chest. 1982 Sep;82(3):266–271. doi: 10.1378/chest.82.3.266. [DOI] [PubMed] [Google Scholar]
  18. Metzger Z., Hoffeld J. T., Oppenheim J. J. Macrophage-mediated suppression. I. Evidence for participation of both hdyrogen peroxide and prostaglandins in suppression of murine lymphocyte proliferation. J Immunol. 1980 Feb;124(2):983–988. [PubMed] [Google Scholar]
  19. Monick M., Glazier J., Hunninghake G. W. Human alveolar macrophages suppress interleukin-1 (IL-1) activity via the secretion of prostaglandin E2. Am Rev Respir Dis. 1987 Jan;135(1):72–77. doi: 10.1164/arrd.1987.135.1.72. [DOI] [PubMed] [Google Scholar]
  20. Nathan C. F., Murray H. W., Cohn Z. A. The macrophage as an effector cell. N Engl J Med. 1980 Sep 11;303(11):622–626. doi: 10.1056/NEJM198009113031106. [DOI] [PubMed] [Google Scholar]
  21. Neumann C., Sorg C. Immune interferon. I. Production by lymphokine-activated murine macrophages. Eur J Immunol. 1977 Oct;7(10):719–725. doi: 10.1002/eji.1830071014. [DOI] [PubMed] [Google Scholar]
  22. Shellito J., Caldwell J. L., Kaltreider H. B. Immune functions of murine alveolar macrophages: binding of lymphocytes and support of lymphocyte proliferation. Exp Lung Res. 1983 Feb;4(2):93–107. doi: 10.3109/01902148309055007. [DOI] [PubMed] [Google Scholar]
  23. Shellito J., Kaltreider H. B. Heterogeneity of immunologic function among subfractions of normal rat alveolar macrophages. Am Rev Respir Dis. 1984 May;129(5):747–753. doi: 10.1164/arrd.1984.129.5.747. [DOI] [PubMed] [Google Scholar]
  24. Todd R. F., 3rd, Reinherz E. L., Schlossman S. F. Human macrophage-lymphocyte interaction in proliferation to soluble antigen. I. Specific delection of lymphocyte proliferative activity on macrophage monolayers. Cell Immunol. 1980 Sep 15;55(1):114–123. doi: 10.1016/0008-8749(80)90142-2. [DOI] [PubMed] [Google Scholar]
  25. Toews G. B., Vial W. C., Dunn M. M., Guzzetta P., Nunez G., Stastny P., Lipscomb M. F. The accessory cell function of human alveolar macrophages in specific T cell proliferation. J Immunol. 1984 Jan;132(1):181–186. [PubMed] [Google Scholar]
  26. Twomey J. J., Laughter A., Brown M. F. A comparison of the regulatory effects of human monocytes, pulmonary alveolar macrophages (PAMs) and spleen macrophages upon lymphocyte responses. Clin Exp Immunol. 1983 May;52(2):449–454. [PMC free article] [PubMed] [Google Scholar]
  27. Wahl S. M., Wahl L. M. Lymphokine modulation of connective tissue metabolism. Ann N Y Acad Sci. 1979;332:411–422. doi: 10.1111/j.1749-6632.1979.tb47135.x. [DOI] [PubMed] [Google Scholar]
  28. Walker W. C., Wright V. Pulmonary lesions and rheumatoid arthritis. Medicine (Baltimore) 1968 Nov;47(6):501–520. doi: 10.1097/00005792-196811000-00003. [DOI] [PubMed] [Google Scholar]
  29. Warnke R., Levy R. Detection of T and B cell antigens hybridoma monoclonal antibodies: a biotin-avidin-horseradish peroxidase method. J Histochem Cytochem. 1980 Aug;28(8):771–776. doi: 10.1177/28.8.7003003. [DOI] [PubMed] [Google Scholar]
  30. Willoughby W. F., Willoughby J. B., Gerberick G. F. Polyclonal activators in pulmonary immune disease. Clin Rev Allergy. 1985 May;3(2):197–216. doi: 10.1007/BF02992983. [DOI] [PubMed] [Google Scholar]
  31. deShazo R. D., Banks D. E., Diem J. E., Nordberg J. A., Baser Y., Bevier D., Salvaggio J. E. Bronchoalveolar lavage cell--lymphocyte interactions in normal nonsmokers and smokers. Analysis with a novel system. Am Rev Respir Dis. 1983 May;127(5):545–548. doi: 10.1164/arrd.1983.127.5.545. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES