Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1988 Apr;72(1):84–90.

Synergy in antigen presentation by thyroid epithelial cells and monocytes from patients with graves' disease

K Eguchi, T Otsubo, Y Kawabe, Chikako Shimomura, Y Ueki, H Nakao, H Tezuka, Mayumi Matsunaga, T Fukuda, N Ishikawa, K Ito, S Nagataki
PMCID: PMC1541511  PMID: 3260842

Abstract

The present study was undertaken to examine the ability of thyrocytes from Graves' patients to present purified protein derivative (PPD) to autologous peripheral blood T cells. Normal human thyrocytes which were pre-cultured with interferon-γ were able to induce the proliferation of T cells in response to PPD antigen, but unstimulated thyrocytes failed to do. Thyrocytes from Graves' patients on which HLA-DR antigens were expressed have an ability to induce the proliferation of T cells. Thyrocytes from Graves' patients which were pulsed with PPD antigen for 4 h were capable of stimulating proliferation of the T cells. However, the stimulation index of T cells co-cultured with thyrocytes and PPD were significantly lower than that of T cells co-cultured with monocytes and PPD. Sub-optimal numbers of monocytes which by themselves were unable to support T-cell proliferation synergistically augmented antigen presentation by thyrocytes. These results suggest that cellular interactions among thyrocytes, monocytes and T cells may perpetuate immune or autoimmune responses in thyroid tissues from Graves' patients.

Full text

PDF
84

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albert F., Hua C., Truneh A., Pierres M., Schmitt-Verhulst A. M. Distinction between antigen receptor and IL 2 receptor triggering events in the activation of alloreactive T cell clones with calcium ionophore and phorbol ester. J Immunol. 1985 Jun;134(6):3649–3655. [PubMed] [Google Scholar]
  2. Arnold A., Lipkowitz S., Suthanthiran M., Novogrodsky A., Stenzel K. H. Human B lymphoblastoid cell lines provide an interleukin 1-like signal for mitogen-treated T lymphocytes via direct cell contact. J Immunol. 1985 Jun;134(6):3876–3881. [PubMed] [Google Scholar]
  3. Chesnut R. W., Colon S. M., Grey H. M. Antigen presentation by normal B cells, B cell tumors, and macrophages: functional and biochemical comparison. J Immunol. 1982 Apr;128(4):1764–1768. [PubMed] [Google Scholar]
  4. Davies T. F. Cocultures of human thyroid monolayer cells and autologous T cells: impact of HLA class II antigen expression. J Clin Endocrinol Metab. 1985 Sep;61(3):418–422. doi: 10.1210/jcem-61-3-418. [DOI] [PubMed] [Google Scholar]
  5. Eguchi K., Otsubo T., Kawabe Y., Ueki Y., Fukuda T., Matsunaga M., Shimomura C., Ishikawa N., Tezuka H., Nakao H. The remarkable proliferation of helper T cell subset in response to autologous thyrocytes and intrathyroidal T cells from patients with Graves' disease. Clin Exp Immunol. 1987 Nov;70(2):403–410. [PMC free article] [PubMed] [Google Scholar]
  6. Erb P., Feldmann M. Role of macrophages in in vitro induction of T-helper cells. Nature. 1975 Mar 27;254(5498):352–354. doi: 10.1038/254352a0. [DOI] [PubMed] [Google Scholar]
  7. Glimcher L. H., Kim K. J., Green I., Paul W. E. Ia antigen-bearing B cell tumor lines can present protein antigen and alloantigen in a major histocompatibility complex-restricted fashion to antigen-reactive T cells. J Exp Med. 1982 Feb 1;155(2):445–459. doi: 10.1084/jem.155.2.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hanafusa T., Pujol-Borrell R., Chiovato L., Russell R. C., Doniach D., Bottazzo G. F. Aberrant expression of HLA-DR antigen on thyrocytes in Graves' disease: relevance for autoimmunity. Lancet. 1983 Nov 12;2(8359):1111–1115. doi: 10.1016/s0140-6736(83)90628-1. [DOI] [PubMed] [Google Scholar]
  9. Hara T., Fu S. M. Human T cell activation. I. Monocyte-independent activation and proliferation induced by anti-T3 monoclonal antibodies in the presence of tumor promoter 12-o-tetradecanoyl phorbol-13 acetate. J Exp Med. 1985 Apr 1;161(4):641–656. doi: 10.1084/jem.161.4.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Issekutz T., Chu E., Geha R. S. Antigen presentation by human B cells: T cell proliferation induced by Epstein Barr virus B lymphoblastoid cells. J Immunol. 1982 Oct;129(4):1446–1450. [PubMed] [Google Scholar]
  11. Jansson R., Karlsson A., Forsum U. Intrathyroidal HLA-DR expression and T lymphocyte phenotypes in Graves' thyrotoxicosis, Hashimoto's thyroiditis and nodular colloid goitre. Clin Exp Immunol. 1984 Nov;58(2):264–272. [PMC free article] [PubMed] [Google Scholar]
  12. Katzen D., Chu E., Terhost C., Leung D. Y., Gesner M., Miller R. A., Geha R. S. Mechanisms of human T cell response to mitogens: IL 2 induces IL 2 receptor expression and proliferation but not IL 2 synthesis in PHA-stimulated T cells. J Immunol. 1985 Sep;135(3):1840–1845. [PubMed] [Google Scholar]
  13. Londei M., Lamb J. R., Bottazzo G. F., Feldmann M. Epithelial cells expressing aberrant MHC class II determinants can present antigen to cloned human T cells. Nature. 1984 Dec 13;312(5995):639–641. doi: 10.1038/312639a0. [DOI] [PubMed] [Google Scholar]
  14. Matsunaga M., Eguchi K., Fukuda T., Kurata A., Tezuka H., Shimomura C., Otsubo T., Ishikawa N., Ito K., Nagataki S. Class II major histocompatibility complex antigen expression and cellular interactions in thyroid glands of Graves' disease. J Clin Endocrinol Metab. 1986 Apr;62(4):723–728. doi: 10.1210/jcem-62-4-723. [DOI] [PubMed] [Google Scholar]
  15. Meuer S. C., Hussey R. E., Cantrell D. A., Hodgdon J. C., Schlossman S. F., Smith K. A., Reinherz E. L. Triggering of the T3-Ti antigen-receptor complex results in clonal T-cell proliferation through an interleukin 2-dependent autocrine pathway. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1509–1513. doi: 10.1073/pnas.81.5.1509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Silberberg-Sinakin I., Gigli I., Baer R. L., Thorbecke G. J. Langerhans cells: role in contact hypersensitivity and relationship to lymphoid dendritic cells and to macrophages. Immunol Rev. 1980;53:203–232. doi: 10.1111/j.1600-065x.1980.tb01045.x. [DOI] [PubMed] [Google Scholar]
  17. Sunshine G. H., Katz D. R., Feldmann M. Dendritic cells induce T cell proliferation to synthetic antigens under Ir gene control. J Exp Med. 1980 Dec 1;152(6):1817–1822. doi: 10.1084/jem.152.6.1817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Todd I., Pujol-Borrell R., Hammond L. J., Bottazzo G. F., Feldmann M. Interferon-gamma induces HLA-DR expression by thyroid epithelium. Clin Exp Immunol. 1985 Aug;61(2):265–273. [PMC free article] [PubMed] [Google Scholar]
  19. Umetsu D. T., Katzen D., Jabara H. H., Geha R. S. Antigen presentation by human dermal fibroblasts: activation of resting T lymphocytes. J Immunol. 1986 Jan;136(2):440–445. [PubMed] [Google Scholar]
  20. Umetsu D. T., Pober J. S., Jabara H. H., Fiers W., Yunis E. J., Burakoff S. J., Reiss C. S., Geha R. S. Human dermal fibroblasts present tetanus toxoid antigen to antigen-specific T cell clones. J Clin Invest. 1985 Jul;76(1):254–260. doi: 10.1172/JCI111955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Van Wauwe J. P., Goossens J. G., Beverley P. C. Human T lymphocyte activation by monoclonal antibodies; OKT3, but not UCHT1, triggers mitogenesis via an interleukin 2-dependent mechanism. J Immunol. 1984 Jul;133(1):129–132. [PubMed] [Google Scholar]
  22. Wakasugi H., Bertoglio J., Tursz T., Fradelizi D. IL 2 receptor induction on human T lymphocytes: role for IL 2 and monocytes. J Immunol. 1985 Jul;135(1):321–327. [PubMed] [Google Scholar]
  23. Weetman A. P., Volkman D. J., Burman K. D., Gerrard T. L., Fauci A. S. The in vitro regulation of human thyrocyte HLA-DR antigen expression. J Clin Endocrinol Metab. 1985 Nov;61(5):817–824. doi: 10.1210/jcem-61-5-817. [DOI] [PubMed] [Google Scholar]
  24. Weiss A., Wiskocil R. L., Stobo J. D. The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL 2 production reflects events occurring at a pre-translational level. J Immunol. 1984 Jul;133(1):123–128. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES