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. 1988 May;72(2):344–349.

Quantitation of human serum polymeric IgA, IgA1 and IgA2 immunoglobulin by enzyme immunoassay.

C Jones 1, N Mermelstein 1, P Kincaid-Smith 1, H Powell 1, D Roberton 1
PMCID: PMC1541545  PMID: 3044652

Abstract

This report concerns the relative quantitation of serum polymeric IgA and polymeric IgA subclass concentrations by enzyme immunoassay (EIA). The assay relies on the specific binding of polymeric IgA to secretory component. Competition between pentameric IgM and polymeric IgA for binding to secretory component was observed. Thus, samples were adsorbed for IgM by affinity chromatography before the EIA was performed. The assay was used to determine an age-related range of serum polymeric IgA concentrations and to compare the polymeric IgA concentrations in patients with IgA nephropathy (n = 50) to those of controls (n = 50). The serum concentrations of both polymeric IgA and polymeric IgA1 increased with age reaching adult values of around 12 years of age. Polymeric IgA2 concentrations did not reach adult levels until 18 years of age. The ratio of the polymeric IgA concentration to the total serum IgA concentration was found to be significantly increased in children under 2 years of age compared with those over 4 years of age (Mann-Whitney U-test, P less than 0.01). Patients with IgA nephropathy had significantly increased concentrations of polymeric IgA (P = 0.001) and polymeric IgA1 (P = 0.001) but similar polymeric IgA2 concentrations to controls.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bene M. C., Faure G., Duheille J. IgA nephropathy: characterization of the polymeric nature of mesangial deposits by in vitro binding of free secretory component. Clin Exp Immunol. 1982 Mar;47(3):527–534. [PMC free article] [PubMed] [Google Scholar]
  2. Brandtzaeg P. Role of J chain and secretory component in receptor-mediated glandular and hepatic transport of immunoglobulins in man. Scand J Immunol. 1985 Aug;22(2):111–146. doi: 10.1111/j.1365-3083.1985.tb01866.x. [DOI] [PubMed] [Google Scholar]
  3. Conley M. E., Arbeter A., Douglas S. D. Serum levels of IgA1 and IgA2 in children and in patients with IgA deficiency. Mol Immunol. 1983 Sep;20(9):977–981. doi: 10.1016/0161-5890(83)90038-x. [DOI] [PubMed] [Google Scholar]
  4. Conley M. E., Cooper M. D., Michael A. F. Selective deposition of immunoglobulin A1 in immunoglobulin A nephropathy, anaphylactoid purpura nephritis, and systemic lupus erythematosus. J Clin Invest. 1980 Dec;66(6):1432–1436. doi: 10.1172/JCI109998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Conley M. E., Delacroix D. L. Intravascular and mucosal immunoglobulin A: two separate but related systems of immune defense? Ann Intern Med. 1987 Jun;106(6):892–899. doi: 10.7326/0003-4819-106-6-892. [DOI] [PubMed] [Google Scholar]
  6. Delacroix D. L., Liroux E., Vaerman J. P. High proportion of polymeric IgA in young infants' sera and independence between IgA-size and IgA-subclass distributions. J Clin Immunol. 1983 Jan;3(1):51–56. doi: 10.1007/BF00919138. [DOI] [PubMed] [Google Scholar]
  7. Egido J., Sancho J., Mampaso F., Lopez Trascasa M., Sanchez Crespo M., Blasco R., Hernando L. A possible common pathogenesis of the mesangial IgA glomerulonephritis in patients with Berger's disease and Schönlein-Henoch syndrome. Proc Eur Dial Transplant Assoc. 1980;17:660–666. [PubMed] [Google Scholar]
  8. Lomax-Smith J. D., Zabrowarny L. A., Howarth G. S., Seymour A. E., Woodroffe A. J. The immunochemical characterization of mesangial IgA deposits. Am J Pathol. 1983 Dec;113(3):359–364. [PMC free article] [PubMed] [Google Scholar]
  9. Radl J., Swart A. C., Mestecky J. The nature of the polymeric serum IgA in man. Proc Soc Exp Biol Med. 1975 Nov;150(2):482–484. doi: 10.3181/00379727-150-39061. [DOI] [PubMed] [Google Scholar]
  10. Russell M. W., Mestecky J., Julian B. A., Galla J. H. IgA-associated renal diseases: antibodies to environmental antigens in sera and deposition of immunoglobulins and antigens in glomeruli. J Clin Immunol. 1986 Jan;6(1):74–86. doi: 10.1007/BF00915367. [DOI] [PubMed] [Google Scholar]
  11. Rádl J., Klein F., van den Berg P., de Bruyn A. M., Hijmans W. Binding of secretory piece to polymeric IgA and IgM paraproteins in vitro. Immunology. 1971 May;20(5):843–852. [PMC free article] [PubMed] [Google Scholar]
  12. Tomino Y., Endoh M., Nomoto Y., Sakai H. Specificity of eluted antibody from renal tissues of patients with IgA nephropathy. Am J Kidney Dis. 1982 Mar;1(5):276–280. doi: 10.1016/s0272-6386(82)80025-5. [DOI] [PubMed] [Google Scholar]
  13. Trascasa M. L., Egido J., Sancho J., Hernando L. IgA glomerulonephritis (Berger's disease): evidence of high serum levels of polymeric IgA. Clin Exp Immunol. 1980 Nov;42(2):247–254. [PMC free article] [PubMed] [Google Scholar]
  14. Valentijn R. M., Radl J., Haaijman J. J., Vermeer B. J., Weening J. J., Kauffmann R. H., Daha M. R., van Es L. A. Circulating and mesangial secretory component-binding IgA-1 in primary IgA nephropathy. Kidney Int. 1984 Nov;26(5):760–766. doi: 10.1038/ki.1984.213. [DOI] [PubMed] [Google Scholar]

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