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. 1988 Jun;72(3):422–427.

Cytokines in synovial fluid. I. The presence of biologically active and immunoreactive IL-1.

S J Hopkins 1, M Humphreys 1, M I Jayson 1
PMCID: PMC1541559  PMID: 3262460

Abstract

Using a sensitive IL-1-dependent T cell clone we estimated interleukin 1 (IL-1)-like activity in a variety of synovial fluids (SF). Although we demonstrate that SF contains potent inhibitors of endogenous and exogenous IL-1 we were able to estimate that samples from rheumatoid arthritis, osteoarthritis and other arthritides contain the equivalent of tens or hundreds of picograms of IL-1 per millilitre, when compared to a standard IL-1 preparation. Although there was some correlation between these results and those obtained using a radio-immunoassay (RIA) for IL-1 beta, the RIA indicated that IL-1 was present in the nanogram range. The results suggest that the importance of inhibitors of IL-1 in SF merits particular attention and that it is also important to consider the bioactivity of cytokines which may be detected by physical methods.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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