Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1988 Aug;73(2):226–229.

Analysis of CD4-positive T cell subpopulation in sarcoidosis.

R Gerli 1, S Darwish 1, L Broccucci 1, V Minotti 1, F Spinozzi 1, C Cernetti 1, A Bertotto 1, P Rambotti 1
PMCID: PMC1541616  PMID: 3263230

Abstract

Double-labelling immunofluorescence analysis within the CD4+ cell subset was carried out in 27 bronchoalveolar lavage fluids and 11 peripheral blood samples of sarcoidosis patients with anti-TQ1, anti-2H4 and anti-4B4 monoclonal antibodies. Helper/inducer CD4+TQ1-/4B4+ cells were strongly increased in the lung and slightly, but significantly, decreased in the blood of sarcoidosis patients with respect to normal controls. No differences were found in the number of both lung and blood CD4+2H4+ cells between sarcoidosis patients and controls. The findings are further evidence for a compartmentalization of T cell subsets in sarcoidosis.

Full text

PDF
226

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer W., Gorny M. K., Baumann H. R., Morell A. T-lymphocyte subsets and immunoglobulin concentrations in bronchoalveolar lavage of patients with sarcoidosis and high and low intensity alveolitis. Am Rev Respir Dis. 1985 Nov;132(5):1060–1065. doi: 10.1164/arrd.1985.132.5.1060. [DOI] [PubMed] [Google Scholar]
  2. Buchalter S., App W., Jackson L., Chandler D., Jackson R., Fulmer J. Bronchoalveolar lavage cell analysis in sarcoidosis. A comparison of lymphocyte counts and clinical course. Ann N Y Acad Sci. 1986;465:678–684. doi: 10.1111/j.1749-6632.1986.tb18545.x. [DOI] [PubMed] [Google Scholar]
  3. Cordier G., Brune J., Revillard J. P. Alveolar lymphocyte activation during sarcoidosis. Ann N Y Acad Sci. 1986;465:140–147. doi: 10.1111/j.1749-6632.1986.tb18489.x. [DOI] [PubMed] [Google Scholar]
  4. Costabel U., Bross K. J., Guzman J., Nilles A., Rühle K. H., Matthys H. Predictive value of bronchoalveolar T cell subsets for the course of pulmonary sarcoidosis. Ann N Y Acad Sci. 1986;465:418–426. doi: 10.1111/j.1749-6632.1986.tb18518.x. [DOI] [PubMed] [Google Scholar]
  5. Gerli R., Bertotto A., Spinozzi F., Cernetti C., Grignani F., Rambotti P. Phenotypic dissection of cord blood immunoregulatory T-cell subsets by using a two-color immunofluorescence study. Clin Immunol Immunopathol. 1986 Sep;40(3):429–435. doi: 10.1016/0090-1229(86)90187-x. [DOI] [PubMed] [Google Scholar]
  6. Hudspith B. N., Flint K. C., Geraint-James D., Brostoff J., Johnson N. M. Lack of immune deficiency in sarcoidosis: compartmentalisation of the immune response. Thorax. 1987 Apr;42(4):250–255. doi: 10.1136/thx.42.4.250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hunninghake G. W., Crystal R. G. Pulmonary sarcoidosis: a disorder mediated by excess helper T-lymphocyte activity at sites of disease activity. N Engl J Med. 1981 Aug 20;305(8):429–434. doi: 10.1056/NEJM198108203050804. [DOI] [PubMed] [Google Scholar]
  8. Israel-Biet D., Venet A., Chrétien J. Persistent high alveolar lymphocytosis as a predictive criterion of chronic pulmonary sarcoidosis. Ann N Y Acad Sci. 1986;465:395–406. doi: 10.1111/j.1749-6632.1986.tb18516.x. [DOI] [PubMed] [Google Scholar]
  9. Keogh B. A., Hunninghake G. W., Line B. R., Crystal R. G. The alveolitis of pulmonary sarcoidosis. Evaluation of natural history and alveolitis-dependent changes in lung function. Am Rev Respir Dis. 1983 Aug;128(2):256–265. doi: 10.1164/arrd.1983.128.2.256. [DOI] [PubMed] [Google Scholar]
  10. Morimoto C., Letvin N. L., Boyd A. W., Hagan M., Brown H. M., Kornacki M. M., Schlossman S. F. The isolation and characterization of the human helper inducer T cell subset. J Immunol. 1985 Jun;134(6):3762–3769. [PubMed] [Google Scholar]
  11. Morimoto C., Letvin N. L., Distaso J. A., Aldrich W. R., Schlossman S. F. The isolation and characterization of the human suppressor inducer T cell subset. J Immunol. 1985 Mar;134(3):1508–1515. [PubMed] [Google Scholar]
  12. NIH conference. Pulmonary sarcoidosis: a disease characterized and perpetuated by activated lung T-lymphocytes. Ann Intern Med. 1981 Jan;94(1):73–94. doi: 10.7326/0003-4819-94-1-73. [DOI] [PubMed] [Google Scholar]
  13. Reinherz E. L., Morimoto C., Fitzgerald K. A., Hussey R. E., Daley J. F., Schlossman S. F. Heterogeneity of human T4+ inducer T cells defined by a monoclonal antibody that delineates two functional subpopulations. J Immunol. 1982 Jan;128(1):463–468. [PubMed] [Google Scholar]
  14. Reinherz E. L., Schlossman S. F. Current concepts in immunology: Regulation of the immune response--inducer and suppressor T-lymphocyte subsets in human beings. N Engl J Med. 1980 Aug 14;303(7):370–373. doi: 10.1056/NEJM198008143030704. [DOI] [PubMed] [Google Scholar]
  15. Reynolds H. Y. Bronchoalveolar lavage. Am Rev Respir Dis. 1987 Jan;135(1):250–263. doi: 10.1164/arrd.1987.135.1.250. [DOI] [PubMed] [Google Scholar]
  16. Rossi G. A., Sacco O., Cosulich E., Risso A., Balbi B., Ravazzoni C. Helper T-lymphocytes in pulmonary sarcoidosis. Functional analysis of a lung T-cell subpopulation in patients with active disease. Am Rev Respir Dis. 1986 Jun;133(6):1086–1090. doi: 10.1164/arrd.1986.133.6.1086. [DOI] [PubMed] [Google Scholar]
  17. Semenzato G., Agostini C., Zambello R., Trentin L., Chilosi M., Angi M. R., Ossi E., Cipriani A., Pizzolo G. Activated T cells with immunoregulatory functions at different sites of involvement in sarcoidosis. Phenotypic and functional evaluations. Ann N Y Acad Sci. 1986;465:56–73. doi: 10.1111/j.1749-6632.1986.tb18481.x. [DOI] [PubMed] [Google Scholar]
  18. Thomas P. D., Hunninghake G. W. Current concepts of the pathogenesis of sarcoidosis. Am Rev Respir Dis. 1987 Mar;135(3):747–760. doi: 10.1164/arrd.1987.135.3.747. [DOI] [PubMed] [Google Scholar]
  19. Wallaert B., Ramon P., Fournier E. C., Prin L., Tonnel A. B., Voisin C. Activated alveolar macrophage and lymphocyte alveolitis in extrathoracic sarcoidosis without radiological mediastinopulmonary involvement. Ann N Y Acad Sci. 1986;465:201–210. doi: 10.1111/j.1749-6632.1986.tb18496.x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES