Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1988 Jan;71(1):126–131.

Natural killer cells in Behçet's disease.

K Hamzaoui 1, K Ayed 1, M Hamza 1, J L Touraine 1
PMCID: PMC1541639  PMID: 3258201

Abstract

We studied natural killer (NK) cell activity and numbers in the peripheral blood obtained from patients with Behçet's disease (BD) in inactive and convalescent stage, and from healthy controls. Ratios of helper/suppressor cells (OKT4/OKT8) were below 1.0 in patients with active stage and were normal in the convalescent stage of BD. A relative increase of OKT8+ cells and at the same time of Leu 7+ cells was obtained in the active and convalescent BD stages. Double marker analysis revealed that the sub-population of cells expressing both the T8+ and the Leu 7+ antigen (T8+/Leu 7+) was increased in patients with active stage, and normal in the convalescent stage. The frequency of cells reactive with Leu 11 monoclonal antibody (active NK cells) was evaluated in patients with BD. Data from peripheral blood showed an increased sub-population of T8+/Leu 7+ double marker cells, and a decreased Leu 11+ cell sub-population in patients with active BD, but the majority of Leu 7+ cells in patients with convalescent stage lacked OKT8 antigen when investigated in a double marker system. A parallel increase of Leu 11+ cells was observed in the convalescent stage. This phenotypic analysis was carried out with the NK in vitro functional evaluation of cell populations from peripheral blood. NK cell activity in the clinically active stage of BD was significantly lower than that of healthy controls and patients in the convalescent stage. The decrease of peripheral blood NK function in patients with active BD may be related to the presence of immature forms of NK cells and/or to the increased percentage of T8+/Leu 7+ cells.

Full text

PDF
126

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abo T., Cooper M. D., Balch C. M. Characterization of HNK-1+ (Leu-7) human lymphocytes. I. Two distinct phenotypes of human NK cells with different cytotoxic capability. J Immunol. 1982 Oct;129(4):1752–1757. [PubMed] [Google Scholar]
  2. Abo T., Miller C. A., Gartland G. L., Balch C. M. Differentiation stages of human natural killer cells in lymphoid tissues from fetal to adult life. J Exp Med. 1983 Jan 1;157(1):273–284. doi: 10.1084/jem.157.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Adinolfi M., Lehner T. Acute phase proteins and C9 in patients with Behcet's syndrome and aphthous ulcers. Clin Exp Immunol. 1976 Jul;25(1):36–39. [PMC free article] [PubMed] [Google Scholar]
  4. Ayed K., Hamzzaoui K., Hamza M., Ben Ayed H. Augmentation de l'activité "Natural Killer" au cours de la maladie de Behçet. Presse Med. 1985 Dec 21;14(45):2299–2300. [PubMed] [Google Scholar]
  5. Bacon T. H., Ozbakir F., Elms C. A., Denman A. M. Interferon-gamma production by peripheral blood mononuclear cells from patients with Behçet's syndrome. Clin Exp Immunol. 1984 Sep;57(3):541–547. [PMC free article] [PubMed] [Google Scholar]
  6. Denman A. M., Fialkow P. J., Pelton B. K., Salo A. C., Appleford D. J., Gilchrist C. Lymphocyte abnormalities in Behçet's syndrome. Clin Exp Immunol. 1980 Oct;42(1):175–185. [PMC free article] [PubMed] [Google Scholar]
  7. Eglin R. P., Lehner T., Subak-Sharpe J. H. Detection of RNA complementary to herpes-simplex virus in mononuclear cells from patients with Behçet's syndrome and recurrent oral ulcers. Lancet. 1982 Dec 18;2(8312):1356–1361. doi: 10.1016/s0140-6736(82)91268-5. [DOI] [PubMed] [Google Scholar]
  8. Gupta R. C., O'Duffy J. D., McDuffie F. C., Meurer M., Jordon R. E. Circulating immune complexes in active Behçet's disease. Clin Exp Immunol. 1978 Nov;34(2):213–218. [PMC free article] [PubMed] [Google Scholar]
  9. Hamza M. H. Treatment of Behçet's disease with thalidomide. Clin Rheumatol. 1986 Sep;5(3):365–371. doi: 10.1007/BF02054255. [DOI] [PubMed] [Google Scholar]
  10. Huwyler T., Morell A., Hirt A., Ricci C., Wagner H. P., Barandun S. Correlation of natural killer cell function with Leu 7 reactivity in patients with humoral immunodeficiency. Clin Exp Immunol. 1984 Jun;56(3):486–492. [PMC free article] [PubMed] [Google Scholar]
  11. Kaneko F., Takahashi Y., Muramatsu R., Adachi K., Miura Y., Nakane A., Minagawa T. Natural killer cell numbers and function in peripheral lymphoid cells in Behcet's disease. Br J Dermatol. 1985 Sep;113(3):313–318. doi: 10.1111/j.1365-2133.1985.tb02083.x. [DOI] [PubMed] [Google Scholar]
  12. Kaneko F., Takahashi Y., Muramatsu Y., Miura Y. Immunological studies on aphthous ulcer and erythema nodosum-like eruptions in Behcet's disease. Br J Dermatol. 1985 Sep;113(3):303–312. doi: 10.1111/j.1365-2133.1985.tb02082.x. [DOI] [PubMed] [Google Scholar]
  13. Lanier L. L., Le A. M., Phillips J. H., Warner N. L., Babcock G. F. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983 Oct;131(4):1789–1796. [PubMed] [Google Scholar]
  14. Lehner T. Characterization of mucosal antibodies in recurrent aphthous ulceration and Behcet's syndrome. Arch Oral Biol. 1969 Jul;14(7):843–853. doi: 10.1016/0003-9969(69)90174-5. [DOI] [PubMed] [Google Scholar]
  15. Lim S. D., Haw C. R., Kim N. I., Fusaro R. M. Abnormalities of T-cell subsets in Behçet's syndrome. Arch Dermatol. 1983 Apr;119(4):307–310. [PubMed] [Google Scholar]
  16. Lohmeyer J., Rieber P., Feucht H., Johnson J., Hadam M., Riethmüller G. A subset of human cells isolated and characterized by monoclonal antibodies. Eur J Immunol. 1981 Dec;11(12):997–1001. doi: 10.1002/eji.1830111209. [DOI] [PubMed] [Google Scholar]
  17. Maccario R., Ugazio A. G., Nespoli L., Alberini C., Montagna D., Porta F., Bonetti F., Burgio G. R. Lymphocyte subpopulations in Down's syndrome: high percentage of circulating HNK-1+, Leu 2a+ cells. Clin Exp Immunol. 1984 Jul;57(1):220–226. [PMC free article] [PubMed] [Google Scholar]
  18. Morimoto C., Reinherz E. L., Nadler L. M., Distaso J. A., Steinberg A. D., Schlossman S. F. Comparison in T- and B-cell markers in patients with Sjögren's syndrome and systemic lupus erythematosus. Clin Immunol Immunopathol. 1982 Feb;22(2):270–278. doi: 10.1016/0090-1229(82)90043-5. [DOI] [PubMed] [Google Scholar]
  19. Ohno S. Immunological aspects of Behçet's and Vogt-Koyanagi-Harada's diseases. Trans Ophthalmol Soc U K. 1981 Sep;101(3):335–341. [PubMed] [Google Scholar]
  20. Ortaldo J. R., Sharrow S. O., Timonen T., Herberman R. B. Determination of surface antigens on highly purified human NK cells by flow cytometry with monoclonal antibodies. J Immunol. 1981 Dec;127(6):2401–2409. [PubMed] [Google Scholar]
  21. Perussia B., Starr S., Abraham S., Fanning V., Trinchieri G. Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. I. Characterization of the lymphocyte subset reactive with B73.1. J Immunol. 1983 May;130(5):2133–2141. [PubMed] [Google Scholar]
  22. Perussia B., Trinchieri G., Jackson A., Warner N. L., Faust J., Rumpold H., Kraft D., Lanier L. L. The Fc receptor for IgG on human natural killer cells: phenotypic, functional, and comparative studies with monoclonal antibodies. J Immunol. 1984 Jul;133(1):180–189. [PubMed] [Google Scholar]
  23. Rumpold H., Kraft D., Gastl G., Huber C. The relationship of HNK-1 (Leu 7) and VEP13 antigens on human cells mediating natural killing. Clin Exp Immunol. 1984 Sep;57(3):703–709. [PMC free article] [PubMed] [Google Scholar]
  24. Sakane T., Kotani H., Takada S., Tsunematsu T. Functional aberration of T cell subsets in patients with Behçet's disease. Arthritis Rheum. 1982 Nov;25(11):1343–1351. doi: 10.1002/art.1780251110. [DOI] [PubMed] [Google Scholar]
  25. Tilden A. B., Abo T., Balch C. M. Suppressor cell function of human granular lymphocytes identified by the HNK-1 (Leu 7) monoclonal antibody. J Immunol. 1983 Mar;130(3):1171–1175. [PubMed] [Google Scholar]
  26. Valesini G., Picardo M., Pastore R., Pivetti P., Serafini U. Circulating immune complexes in Behçet's syndrome: purification, characterization and cross-reactivity studies. Clin Exp Immunol. 1981 Jun;44(3):522–527. [PMC free article] [PubMed] [Google Scholar]
  27. Victorino R. M., Ryan P., Hughes G. R., Hodgson H. J. Cell-mediated immune functions and immunoregulatory cells in Behçet's syndrome. Clin Exp Immunol. 1982 Apr;48(1):121–128. [PMC free article] [PubMed] [Google Scholar]
  28. Williams B. D., Lehner T. Immune complexes in Behçet's syndrome and recurrent oral ulceration. Br Med J. 1977 May 28;1(6073):1387–1389. doi: 10.1136/bmj.1.6073.1387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Zarling J. M., Clouse K. A., Biddison W. E., Kung P. C. Phenotypes of human natural killer cell populations detected with monoclonal antibodies. J Immunol. 1981 Dec;127(6):2575–2580. [PubMed] [Google Scholar]
  30. Ziegler-Heitbrock H. W., Schramm W., Stachel D., Rumpold H., Kraft D., Wernicke D., von der Helm K., Eberle J., Deinhardt F., Rieber E. P. Expansion of a minor subpopulation of peripheral blood lymphocytes (T8+/Leu 7+) in patients with haemophilia. Clin Exp Immunol. 1985 Sep;61(3):633–641. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES