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. 1988 Jan;71(1):202–206.

Regulation of Fc epsilon receptor expression on a human monoblast cell line U937.

M Mayumi 1, T Kawabe 1, K M Kim 1, T Heike 1, K Katamura 1, J Yodoi 1, H Mikawa 1
PMCID: PMC1541644  PMID: 2964957

Abstract

Fc epsilon receptor (Fc epsilon R) expression on several human cell lines (U937, RPMI 8866, HL 60, THP-1, and Molt 4) and its regulation were examined by immunofluorescent analysis using a monoclonal anti-human Fc epsilon R antibody, H107. Phorbol ester (PMA), recombinant gamma interferon (IFN-gamma) and H107 itself enhanced Fc epsilon R expression on a FC epsilon R positive cell line U937, whereas these reagents did not induce FC epsilon R expression on the Fc epsilon R negative cell lines, Molt 4, HL 60 and THP-1. Dexamethasone not only suppressed by 50% the spontaneous Fc epsilon R expression on U937 cells but also completely inhibited the enhancement of their Fc epsilon R expression on U937 cells induced by PMA, IFN-gamma or H107. Dexamethasone caused a little suppression of Fc epsilon R expression by RPMI 8866 cells. The results showed that Fc epsilon R expression on a human monoblast cell line U937 was up- or down-regulated by a variety of physiological or pharmacological agents. These experimental systems provide a good model for the investigation of the regulatory mechanisms of Fc epsilon R expression.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adachi M., Okumura K., Watanabe N., Noro N., Masuda T., Yodoi J. Lack of Fc receptor for IgE in SJA9 mice. Immunogenetics. 1985;22(1):77–83. doi: 10.1007/BF00430596. [DOI] [PubMed] [Google Scholar]
  2. Anderson C. L., Abraham G. N. Characterization of the Fc receptor for IgG on a human macrophage cell line, U937. J Immunol. 1980 Dec;125(6):2735–2741. [PubMed] [Google Scholar]
  3. Anderson C. L., Spiegelberg H. L. Macrophage receptors for IgE: binding of IgE to specific IgE Fc receptors on a human macrophage cell line, U937. J Immunol. 1981 Jun;126(6):2470–2473. [PubMed] [Google Scholar]
  4. Broxmeyer H. E., Piacibello W., Juliano L., Platzer E., Berman E., Rubin B. Y. Gamma interferon induces colony-forming cells of the human monoblast cell line U937 to respond to inhibition by lactoferrin, transferrin, and acidic isoferritins. Exp Hematol. 1986 Jan;14(1):35–43. [PubMed] [Google Scholar]
  5. Daeron M., Ishizaka K. Induction of Fc epsilon receptors on mouse macrophages and lymphocytes by homologous IgE. J Immunol. 1986 Mar 1;136(5):1612–1619. [PubMed] [Google Scholar]
  6. Delespesse G., Sarfati M., Rubio-Trujillo M., Wolowiec T. IgE receptors on human lymphocytes. II. Detection of cells bearing IgE receptors in unstimulated mononuclear cells by means of a monoclonal antibody. Eur J Immunol. 1986 Jul;16(7):815–821. doi: 10.1002/eji.1830160716. [DOI] [PubMed] [Google Scholar]
  7. Gonzalez-Molina A., Spiegelberg H. L. A subpopulation of normal human peripheral B lymphcytes that bind IgE. J Clin Invest. 1977 Apr;59(4):616–624. doi: 10.1172/JCI108679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hsu S. M., Hsu P. L. Phenotypes and phorbol ester-induced differentiation of human histiocytic lymphoma cell lines (U-937 and SU-DHL-1) and Reed-Sternberg cells. Am J Pathol. 1986 Feb;122(2):223–230. [PMC free article] [PubMed] [Google Scholar]
  9. Huff T. F., Ishizaka K. Formation of IgE-binding factors by human T-cell hybridomas. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1514–1518. doi: 10.1073/pnas.81.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Huff T. F., Yodoi J., Uede T., Ishizaka K. Presence of an antigenic determinant common to rat IgE-potentiating factor, IgE-suppressive factor, and Fc epsilon receptors on T and B lymphocytes. J Immunol. 1984 Jan;132(1):406–412. [PubMed] [Google Scholar]
  11. Ikuta K., Takami M., Kim C. W., Honjo T., Miyoshi T., Tagaya Y., Kawabe T., Yodoi J. Human lymphocyte Fc receptor for IgE: sequence homology of its cloned cDNA with animal lectins. Proc Natl Acad Sci U S A. 1987 Feb;84(3):819–823. doi: 10.1073/pnas.84.3.819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ishizaka K., Sandberg K. Formation of IgE binding factors by human T lymphocytes. J Immunol. 1981 May;126(5):1692–1696. [PubMed] [Google Scholar]
  13. Kim K. M., Tanaka M., Yoshimura T., Katamura K., Mayumi M., Mikawa H. Regulation of IgE receptor expression on human peripheral blood lymphocytes by lymphocytosis promoting factor (LPF), lectins and dexamethasone. Clin Exp Immunol. 1987 May;68(2):418–426. [PMC free article] [PubMed] [Google Scholar]
  14. Marcelletti J. F., Katz D. H. FcR epsilon+ lymphocytes and regulation of the IgE antibody system. IV. Delineation of target cells and mechanisms of action of SFA and EFA in inhibiting in vitro induction of FcR epsilon expression. J Immunol. 1984 Dec;133(6):2845–2851. [PubMed] [Google Scholar]
  15. Melewicz F. M., Plummer J. M., Spiegelberg H. L. Comparison of the Fc receptors for IgE on human lymphocytes and monocytes. J Immunol. 1982 Aug;129(2):563–569. [PubMed] [Google Scholar]
  16. Melewicz F. M., Spiegelberg H. L. Fc receptors for IgE on a subpopulation of human peripheral blood monocytes. J Immunol. 1980 Sep;125(3):1026–1031. [PubMed] [Google Scholar]
  17. Nagai T., Adachi M., Noro N., Yodoi J., Uchino H. T and B lymphocytes with immunoglobulin E Fc receptors (Fc epsilon R) in patients with nonallergic hyperimmunoglobulinemia E: demonstration using a monoclonal antibody against Fc epsilon R-associated antigen. Clin Immunol Immunopathol. 1985 Jun;35(3):261–275. doi: 10.1016/0090-1229(85)90088-1. [DOI] [PubMed] [Google Scholar]
  18. Noro N., Yoshioka A., Adachi M., Yasuda K., Masuda T., Yodoi J. Monoclonal antibody (H107) inhibiting IgE binding to Fc epsilon R(+) human lymphocytes. J Immunol. 1986 Aug 15;137(4):1258–1263. [PubMed] [Google Scholar]
  19. Náray-Fejes-Tóth A., Cornwell G. G., Guyre P. M. Glucocorticoids inhibit IgE receptor expression on the human monocyte cell line U937. Immunology. 1985 Oct;56(2):359–366. [PMC free article] [PubMed] [Google Scholar]
  20. Náray-Fejes-Tóth A., Guyre P. M. Recombinant human immune interferon induces increased IgE receptor expression on the human monocyte cell line U-937. J Immunol. 1984 Oct;133(4):1914–1919. [PubMed] [Google Scholar]
  21. Sarfati M., Rector E., Wong K., Rubio-Trujillo M., Sehon A. H., Delespesse G. In vitro synthesis of IgE by human lymphocytes. II. Enhancement of the spontaneous IgE synthesis by IgE-binding factors secreted by RPMI 8866 lymphoblastoid B cells. Immunology. 1984 Oct;53(2):197–205. [PMC free article] [PubMed] [Google Scholar]
  22. Spiegelberg H. L., O'Connor R. D., Simon R. A., Mathison D. A. Lymphocytes with immunoglobulin E Fc receptors in patients with atopic disorders. J Clin Invest. 1979 Sep;64(3):714–720. doi: 10.1172/JCI109514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Suemura M., Kikutani H., Barsumian E. L., Hattori Y., Kishimoto S., Sato R., Maeda A., Nakamura H., Owaki H., Hardy R. R. Monoclonal anti-Fc epsilon receptor antibodies with different specificities and studies on the expression of Fc epsilon receptors on human B and T cells. J Immunol. 1986 Aug 15;137(4):1214–1220. [PubMed] [Google Scholar]
  24. Yodoi J., Hirashima M., Hirata F., DeBlas A. L., Ishizaka K. Lymphocytes bearing Fc receptors for IgE. VII. Possible participation of phospholipase A2 in the glycosylation of IgE-binding factors. J Immunol. 1981 Aug;127(2):476–482. [PubMed] [Google Scholar]
  25. Yodoi J., Hirashima M., Ishizaka K. Lymphocytes bearing Fc receptors for IgE. VI. Suppressive effect of glucocorticoids on the expression of Fc epsilon receptors and glycosylation of IgE-binding factors. J Immunol. 1981 Aug;127(2):471–476. [PubMed] [Google Scholar]
  26. Yodoi J., Ishizaka K. Induction of Fc epsilon-receptor bearing cells in vitro in human peripheral lymphocytes. J Immunol. 1980 Feb;124(2):934–938. [PubMed] [Google Scholar]
  27. Yodoi J., Ishizaka K. Lymphocytes bearing Fc receptors for IgE. I. Presence of human and rat T lymphocytes with Fc epsilon receptors. J Immunol. 1979 Jun;122(6):2577–2583. [PubMed] [Google Scholar]
  28. Yodoi J., Ishizaka T., Ishizaka K. Lymphocytes bearing Fc receptors for IgE. II. Induction of Fcepsilon-receptor bearing rat lymphocytes by IgE. J Immunol. 1979 Jul;123(1):455–462. [PubMed] [Google Scholar]

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