Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1988 Jan;71(1):73–78.

Human repetitive and unique sequences coexist in a large circulating DNA species found in cryoprecipitates from SLE patients.

C E Contreras 1, M S Rieber 1, N Bianco 1, M Rieber 1
PMCID: PMC1541645  PMID: 2832108

Abstract

Cryoprecipitates from systemic lupus erythematosus (SLE) patients with high levels of anti DNA antibodies show a sharply migrating large circulating DNA species of about 17-20 kb (M. Rieber et al., Clin. exp. Immunol. (1986) 66, 61). We have now used Southern blot analysis of circulating DNA from different individuals to analyse the relative cross-hybridization of circulating DNA from different individuals, as well as their homology with genomic DNA from different species. Molecular hybridization showed significant homology of the various circulating DNA examined, only with human genomic DNA, but limited cross-reactivity among circulating DNA from different individuals. This suggests that the circulating DNA is composed of sequences repeated in human genomic DNA and by specific sequences unique to circulating DNA from some individuals. Our data suggests the possibility of using probes derived from the specific sequences now reported in the circulating DNA, in gene typing and in the analysis of susceptibility to disease.

Full text

PDF
73

Images in this article

76Fig. 4
on p.76
77Fig. 5
on p.77

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burdick G., Emlen W. Effect of antibody excess on the size, stoichiometry, and DNAse resistance of DNA anti-DNA immune complexes. J Immunol. 1985 Oct;135(4):2593–2597. [PubMed] [Google Scholar]
  2. Contreras C. E., Orozco A., Sánchez P., Ortega G., Bianco N. E. Physiological aspects of circulating immune complexes in the normal population. Clin Exp Immunol. 1982 Jun;48(3):693–699. [PMC free article] [PubMed] [Google Scholar]
  3. Emlen W., Mannik M. Effect of DNA size and strandedness on the in vivo clearance and organ localization of DNA. Clin Exp Immunol. 1984 Apr;56(1):185–192. [PMC free article] [PubMed] [Google Scholar]
  4. Emlen W., Mannik M. Kinetics and mechanisms for removal of circulating single-stranded DNA in mice. J Exp Med. 1978 Mar 1;147(3):684–699. doi: 10.1084/jem.147.3.684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ikebe K., Gupta R. C., Tan E. M. Characterization of DNA in polyethylene glycol precipitated immune complexes from sera of patients with systemic lupus erythematosus. Clin Exp Immunol. 1983 Oct;54(1):169–177. [PMC free article] [PubMed] [Google Scholar]
  6. Impraim C. C., Conner B. J., Klotz J. L., Lee T. E., Teplitz R. L. A method for binding specificity analysis of anti-DNA autoantibodies in SLE. J Immunol Methods. 1985 Apr 22;78(2):191–198. doi: 10.1016/0022-1759(85)90075-4. [DOI] [PubMed] [Google Scholar]
  7. Jeffreys A. J., Wilson V., Thein S. L. Hypervariable 'minisatellite' regions in human DNA. Nature. 1985 Mar 7;314(6006):67–73. doi: 10.1038/314067a0. [DOI] [PubMed] [Google Scholar]
  8. Montgomery K. T., Biedler J. L., Spengler B. A., Melera P. W. Specific DNA sequence amplification in human neuroblastoma cells. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5724–5728. doi: 10.1073/pnas.80.18.5724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Morimoto C., Sano H., Abe T., Homma M., Steinberg A. D. Correlation between clinical activity of systemic lupus erythematosus and the amounts of DNA in DNA/anti-DNA antibody immune complexes. J Immunol. 1982 Nov;129(5):1960–1965. [PubMed] [Google Scholar]
  10. Orozco A., Contreras C. E., Sánchez P., Meilijson O., Bianco N. E. Solid phase C1q microassay for the rapid detection of circulating immune complexes. J Immunol Methods. 1983 Apr 29;59(2):237–243. doi: 10.1016/0022-1759(83)90036-4. [DOI] [PubMed] [Google Scholar]
  11. Rieber M., Contreras C. E., Rieber M. S., Bianco N. E. Novel DNA-protein complex and a large DNA in SLE cryoprecipitates. Clin Exp Immunol. 1986 Oct;66(1):61–67. [PMC free article] [PubMed] [Google Scholar]
  12. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  13. Roberts J. L., Robinson M. F., Lewis E. J. Low-molecular-weight plasma cryoprecipitable antinative DNA: polynucleotide complexes in lupus glomerulonephritis. Clin Immunol Immunopathol. 1981 Apr;19(1):75–90. doi: 10.1016/0090-1229(81)90049-0. [DOI] [PubMed] [Google Scholar]
  14. Sano H., Imokawa M., Steinberg A. D., Morimoto C. Accumulation of guanine-cytosine-enriched low M.W. DNA fragments in lymphocytes of patients with systemic lupus erythematosus. J Immunol. 1983 Jan;130(1):187–190. [PubMed] [Google Scholar]
  15. Sano H., Morimoto C. Dna isolated from DNA/anti-DNA antibody immune complexes in systemic lupus erythematosus is rich in guanine-cytosine content. J Immunol. 1982 Mar;128(3):1341–1345. [PubMed] [Google Scholar]
  16. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  17. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]
  18. Van Helden P. D. Potential Z-DNA-forming elements in serum DNA from human systemic lupus erythematosus. J Immunol. 1985 Jan;134(1):177–179. [PubMed] [Google Scholar]
  19. Winfield J. B., Koffler D., Kunkel H. G. Specific concentration of polynucleotide immune complexes in the cryoprecipitates of patients with systemic lupus erythematosus. J Clin Invest. 1975 Sep;56(3):563–570. doi: 10.1172/JCI108125. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES