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. 1988 Jan;71(1):132–137.

Immunohistological analysis of experimental gingivitis in humans.

G J Seymour 1, E Gemmell 1, L J Walsh 1, R N Powell 1
PMCID: PMC1541652  PMID: 3280178

Abstract

Immunohistological analysis of experimental gingivitis in humans was carried out to provide a baseline for the study of immunoregulatory mechanisms in chronic inflammatory periodontal disease. Using a panel of monoclonal antibodies in an avidin biotin immunoperoxidase technique, T cell subsets were identified and the pattern of Class II major histocompatibility complex (MHC) antigens determined. Twenty third-year dental students took part in the study. Following the cessation of oral hygiene procedures, gingival biopsies were taken from each of five students at days 0, 4, 8 and 21 during the development of the inflammatory lesion. Each student had one biopsy which healed uneventfully. The T4:T8 ratio showed only slight variation over the time course of the lesion varying from 2.18:1 at day 0 to 2.48:1 at day 4. At all stages the T cells displayed both HLA-DR and HLA-DQ antigens, but less than 10% had detectable IL-2 receptors. The predominant macrophage population was acid phosphatase + ve, adenosine triphosphatase -ve, HLA-DR+ and HLA-DQ+ antigens suggesting an activated phagocytic population. During the development of the lesion, the number of intraepithelial Langerhans cells (T6+) increased but there appeared to be a discrepancy between HLA-DR and HLA-DQ expression on these cells. Similarly, the keratinocytes expressed HLA-DR but failed to express HLA-DQ at any stage. These results suggest that the developing gingival lesion is a well controlled lesion and follows a similar pattern to a controlled delayed type hypersensitivity (DTH) response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bird P. S., Seymour G. J. Production of monoclonal antibodies that recognize specific and cross-reactive antigens of Fusobacterium nucleatum. Infect Immun. 1987 Mar;55(3):771–777. doi: 10.1128/iai.55.3.771-777.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bosman C. W., Powell R. N. The reversal of localized experimental gingivitis. A comparison between mechanical toothbrushing procedures and a 0.2% chlorhexidine mouthrinse. J Clin Periodontol. 1977 Aug;4(3):161–172. doi: 10.1111/j.1600-051x.1977.tb02270.x. [DOI] [PubMed] [Google Scholar]
  3. Carpenter A. B., Sully E. C., Ranney R. R., Bick P. H. T-cell regulation of polyclonal B-cell activation induced by extracts of oral bacteria associated with periodontal diseases. Infect Immun. 1984 Jan;43(1):326–336. doi: 10.1128/iai.43.1.326-336.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chen Y. X., Evans R. L., Pollack M. S., Lanier L. L., Phillips J. H., Rousso C., Warner N. L., Brodsky F. M. Characterization and expression of the HLA-DC antigens defined by anti-Leu 10. Hum Immunol. 1984 Aug;10(4):221–235. doi: 10.1016/0198-8859(84)90088-0. [DOI] [PubMed] [Google Scholar]
  5. Cole K. L., Seymour G. J., Powell R. N. Phenotypic and functional analysis of T cells extracted from chronically inflamed human periodontal tissues. J Periodontol. 1987 Aug;58(8):569–573. doi: 10.1902/jop.1987.58.8.569. [DOI] [PubMed] [Google Scholar]
  6. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  7. Horton J. E., Leikin S., Oppenheim J. J. Human lymphoproliferative reaction to saliva and dental plaque-deposits: an in vitro correlation with periodontal disease. J Periodontol. 1972 Sep;43(9):522–527. doi: 10.1902/jop.1972.43.9.522. [DOI] [PubMed] [Google Scholar]
  8. Ivanyi L., Lehner T. Lymphocyte transformation by sonicates of dental plaque in human periodontal disease. Arch Oral Biol. 1971 Sep;16(9):1117–1121. doi: 10.1016/0003-9969(71)90216-0. [DOI] [PubMed] [Google Scholar]
  9. Ivanyi L., Lehner T. Stimulation of lymphocyte transformation by bacterial antigens in patients with periodontal disease. Arch Oral Biol. 1970 Nov;15(11):1089–1096. doi: 10.1016/0003-9969(70)90121-4. [DOI] [PubMed] [Google Scholar]
  10. Johannessen A. C., Nilsen R., Knudsen G. E., Kristoffersen T. In situ characterization of mononuclear cells in human chronic marginal periodontitis using monoclonal antibodies. J Periodontal Res. 1986 Mar;21(2):113–127. doi: 10.1111/j.1600-0765.1986.tb01444.x. [DOI] [PubMed] [Google Scholar]
  11. Kung P., Goldstein G., Reinherz E. L., Schlossman S. F. Monoclonal antibodies defining distinctive human T cell surface antigens. Science. 1979 Oct 19;206(4416):347–349. doi: 10.1126/science.314668. [DOI] [PubMed] [Google Scholar]
  12. LOE H., THEILADE E., JENSEN S. B. EXPERIMENTAL GINGIVITIS IN MAN. J Periodontol. 1965 May-Jun;36:177–187. doi: 10.1902/jop.1965.36.3.177. [DOI] [PubMed] [Google Scholar]
  13. Lampson L. A., Levy R. Two populations of Ia-like molecules on a human B cell line. J Immunol. 1980 Jul;125(1):293–299. [PubMed] [Google Scholar]
  14. Landay A., Gartland G. L., Clement L. T. Characterization of a phenotypically distinct subpopulation of Leu-2+ cells that suppresses T cell proliferative responses. J Immunol. 1983 Dec;131(6):2757–2761. [PubMed] [Google Scholar]
  15. Linder J. E., Hopps R. M., Johnson N. W. Methyl green-pyronin with hematoxylin and orange G for the identification of inflammatory cells in tissue sections. Stain Technol. 1977 Jul;52(4):233–236. doi: 10.3109/10520297709116781. [DOI] [PubMed] [Google Scholar]
  16. Löe H. The Gingival Index, the Plaque Index and the Retention Index Systems. J Periodontol. 1967 Nov-Dec;38(6 Suppl):610–616. doi: 10.1902/jop.1967.38.6.610. [DOI] [PubMed] [Google Scholar]
  17. Mackler B. F., Altman L. C., Wahl S., Rosenstreich D. L., Oppenheim J. J., Mergenhagen S. E. Blastogenesis and lymphokine synthesis by T and B lymphocytes from patients with periodontal disease. Infect Immun. 1974 Oct;10(4):844–850. doi: 10.1128/iai.10.4.844-850.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Murphy G. F., Bhan A. K., Sato S., Harrist T. J., Mihm M. C., Jr Characterization of Langerhans cells by the use of monoclonal antibodies. Lab Invest. 1981 Nov;45(5):465–468. [PubMed] [Google Scholar]
  19. Poulter L. W. Antigen presenting cells in situ: their identification and involvement in immunopathology. Clin Exp Immunol. 1983 Sep;53(3):513–520. [PMC free article] [PubMed] [Google Scholar]
  20. Poulter L. W., Seymour G. J., Duke O., Janossy G., Panayi G. Immunohistological analysis of delayed-type hypersensitivity in man. Cell Immunol. 1982 Dec;74(2):358–369. doi: 10.1016/0008-8749(82)90036-3. [DOI] [PubMed] [Google Scholar]
  21. Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. A monoclonal antibody with selective reactivity with functionally mature human thymocytes and all peripheral human T cells. J Immunol. 1979 Sep;123(3):1312–1317. [PubMed] [Google Scholar]
  22. Seymour G. J., Powell R. N., Aitken J. F. Experimental gingivitis in humans. A clinical and histologic investigation. J Periodontol. 1983 Sep;54(9):522–528. doi: 10.1902/jop.1983.54.9.522. [DOI] [PubMed] [Google Scholar]
  23. Seymour G. J., Powell R. N., Cole K. L., Aitken J. F., Brooks D., Beckman I., Zola H., Bradley J., Burns G. F. Experimental gingivitis in humans. A histochemical and immunological characterization of the lymphoid cell subpopulations. J Periodontal Res. 1983 Jul;18(4):375–385. doi: 10.1111/j.1600-0765.1983.tb00373.x. [DOI] [PubMed] [Google Scholar]
  24. Steeg P. S., Moore R. N., Johnson H. M., Oppenheim J. J. Regulation of murine macrophage Ia antigen expression by a lymphokine with immune interferon activity. J Exp Med. 1982 Dec 1;156(6):1780–1793. doi: 10.1084/jem.156.6.1780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Steeg P. S., Moore R. N., Oppenheim J. J. Regulation of murine macrophage Ia-antigen expression by products of activated spleen cells. J Exp Med. 1980 Dec 1;152(6):1734–1744. doi: 10.1084/jem.152.6.1734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Taubman M. A., Stoufi E. D., Ebersole J. L., Smith D. J. Phenotypic studies of cells from periodontal disease tissues. J Periodontal Res. 1984 Nov;19(6):587–590. doi: 10.1111/j.1600-0765.1984.tb01320.x. [DOI] [PubMed] [Google Scholar]
  27. Uchiyama T., Broder S., Waldmann T. A. A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. I. Production of anti-Tac monoclonal antibody and distribution of Tac (+) cells. J Immunol. 1981 Apr;126(4):1393–1397. [PubMed] [Google Scholar]
  28. Walsh L. J., Seymour G. J., Powell R. N. Modulation of class II (DR and DQ) antigen expression on gingival Langerhans cells in vitro by gamma interferon and prostaglandin E2. J Oral Pathol. 1986 Jul;15(6):347–351. doi: 10.1111/j.1600-0714.1986.tb00638.x. [DOI] [PubMed] [Google Scholar]
  29. Walsh L. J., Seymour G. J., Savage N. W. Oral mucosal Langerhans cells express DR and DQ antigens. J Dent Res. 1986 Mar;65(3):390–393. doi: 10.1177/00220345860650030301. [DOI] [PubMed] [Google Scholar]
  30. Yoshie H., Taubman M. A., Ebersole J. L., Smith D. J., Olson C. L. Periodontal bone loss and immune characteristics of congenitally athymic and thymus cell-reconstituted athymic rats. Infect Immun. 1985 Nov;50(2):403–408. doi: 10.1128/iai.50.2.403-408.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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