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. 1988 Jan;71(1):62–66.

Anti-idiotypic induced suppression of Sjögren's syndrome associated anti-La autoantibody secretion in vitro.

A C Horsfall 1, P A Mumford 1, P J Venables 1, R N Maini 1
PMCID: PMC1541658  PMID: 3258205

Abstract

Peripheral blood mononuclear cells from patients with Sjögren's Syndrome spontaneously secrete autoantibodies to the La antigen when cultured in vitro. This paper reports that specific IgG autoantibody production in vitro is suppressed by pre-treatment of CD8+ enriched T cells with rabbit polyclonal antibodies to idiotypes borne by circulating autologous anti-La antibodies. Treatment of this T cell subpopulation with anti-idiotypes specific for circulating anti-La antibodies from other patients or for anti-DNA antibodies was without effect on anti-La antibody production. Similarly anti-La anti-idiotypes had no effect on the production of autoantibodies to other ribonucleoprotein antigens such as nRNP/Sm. These data show that CD8+ T cells are the main targets for anti-idiotypic control in vitro. We suggest that the relative deficit of these cells, plus a surfeit of CD4+ T cells at the site of the pathological lesion within the salivary gland permits localized production of autoantibodies. Thus, dysregulation of the idiotypic network could contribute to the pathogenesis of Sjögren's Syndrome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdou N. I., Wall H., Lindsley H. B., Halsey J. F., Suzuki T. Network theory in autoimmunity. In vitro suppression of serum anti-DNA antibody binding to DNA by anti-idiotypic antibody in systemic lupus erythematosus. J Clin Invest. 1981 May;67(5):1297–1304. doi: 10.1172/JCI110158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alspaugh M. A., Talal N., Tan E. M. Differentiation and characterization of autoantibodies and their antigens in Sjögren's syndrome. Arthritis Rheum. 1976 Mar-Apr;19(2):216–222. doi: 10.1002/art.1780190214. [DOI] [PubMed] [Google Scholar]
  3. Dobloug J. H., Førre O., Natvig J. B., Michaelsen T. E. Demonstration of rheumatoid factor idiotypic antigens on peripheral blood B and T lymphocytes from patients with rheumatoid arthritis. Scand J Immunol. 1979 Mar;9(3):273–279. doi: 10.1111/j.1365-3083.1979.tb02731.x. [DOI] [PubMed] [Google Scholar]
  4. Eichmann K. Expression and function of idiotypes of lymphocytes. Adv Immunol. 1978;26:195–254. doi: 10.1016/s0065-2776(08)60231-x. [DOI] [PubMed] [Google Scholar]
  5. Fong S., Gilbertson T. A., Chen P. P., Vaughan J. H., Carson D. A. Modulation of human rheumatoid factor-specific lymphocyte responses with a cross-reactive anti-idiotype bearing the internal image of antigen. J Immunol. 1984 Mar;132(3):1183–1189. [PubMed] [Google Scholar]
  6. Fox R. I., Hugli T. E., Lanier L. L., Morgan E. L., Howell F. Salivary gland lymphocytes in primary Sjogren's syndrome lack lymphocyte subsets defined by Leu-7 and Leu-11 antigens. J Immunol. 1985 Jul;135(1):207–214. [PubMed] [Google Scholar]
  7. Hahn B. H., Ebling F. M. Suppression of murine lupus nephritis by administration of an anti-idiotypic antibody to anti-DNA. J Immunol. 1984 Jan;132(1):187–190. [PubMed] [Google Scholar]
  8. Isenberg D. A., Rowe D., Tookman A., Hopp A., Griffiths M., Paice E., Stewart J., Beverley P. C. An immunohistological study of secondary Sjögren's syndrome. Ann Rheum Dis. 1984 Jun;43(3):470–476. doi: 10.1136/ard.43.3.470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kojima K., Matsuyama T., Tanaka H. Suppression of in vitro human antithyroglobulin antibody secretion by private and cross-reactive anti-idiotypic antibodies. Clin Immunol Immunopathol. 1986 May;39(2):337–344. doi: 10.1016/0090-1229(86)90097-8. [DOI] [PubMed] [Google Scholar]
  10. Lefvert A. K., Sundén H., Holm G. Acetylcholine receptor antibodies and anti-idiotypic antibodies produced in blood lymphocyte cultures from patients with myasthenia gravis. Scand J Immunol. 1986 Jun;23(6):655–662. doi: 10.1111/j.1365-3083.1986.tb02001.x. [DOI] [PubMed] [Google Scholar]
  11. Lindahl G., Hedfors E., Klareskog L., Forsum U. Epithelial HLA-DR expression and T lymphocyte subsets in salivary glands in Sjögren's syndrome. Clin Exp Immunol. 1985 Sep;61(3):475–482. [PMC free article] [PubMed] [Google Scholar]
  12. Martínez C., Bragado R., de la Hera A., Toribio M. L., Marcos M. A., Bandeira A., Pereira P., Coutinho A. Functional and biochemical evidence for the recognition of T cell receptors by monoclonal antibodies to an immunoglobulin idiotype. J Mol Cell Immunol. 1986;2(6):307–313. [PubMed] [Google Scholar]
  13. Mattioli M., Reichlin M. Heterogeneity of RNA protein antigens reactive with sera of patients with systemic lupus erythematosus. Description of a cytoplasmic nonribosomal antigen. Arthritis Rheum. 1974 Jul-Aug;17(4):421–429. doi: 10.1002/art.1780170413. [DOI] [PubMed] [Google Scholar]
  14. Mumford P., Room G. R., Venables P. J., Maini R. N. IgG antibodies to SS-B (La), RNP/Sm and DNA are produced by PWM-stimulated normal human lymphocytes in culture. Rheumatol Int. 1985;5(3):109–112. doi: 10.1007/BF00541329. [DOI] [PubMed] [Google Scholar]
  15. Pasquali J. L., Urlacher A., Storck D. Idiotypic network: possible explanation of seronegativity in a patient with rheumatoid arthritis. Clin Exp Immunol. 1984 Feb;55(2):281–286. [PMC free article] [PubMed] [Google Scholar]
  16. Rodriguez M. A., Baroja M. L., Leon-Ponte M., Abadi I. Abnormal immunoglobulin and rheumatoid factor synthesis by blood lymphocytes in patients with primary Sjögren's syndrome. Arthritis Rheum. 1986 Dec;29(12):1446–1455. doi: 10.1002/art.1780291205. [DOI] [PubMed] [Google Scholar]
  17. Shoenfeld Y., Isenberg D. A., Rauch J., Madaio M. P., Stollar B. D., Schwartz R. S. Idiotypic cross-reactions of monoclonal human lupus autoantibodies. J Exp Med. 1983 Sep 1;158(3):718–730. doi: 10.1084/jem.158.3.718. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Teitelbaum D., Rauch J., Stollar B. D., Schwartz R. S. In vivo effects of antibodies against a high frequency idiotype of anti-DNA antibodies in MRL mice. J Immunol. 1984 Mar;132(3):1282–1285. [PubMed] [Google Scholar]
  19. Venables P. J., Charles P. J., Buchanan R. R., Yi T., Mumford P. A., Schrieber L., Room G. R., Maini R. N. Quantitation and detection of isotypes of anti-SS-B antibodies by ELISA and Farr assays using affinity purified antigens. An approach to the investigation of Sjögren's syndrome and systemic lupus erythematosus. Arthritis Rheum. 1983 Feb;26(2):146–155. doi: 10.1002/art.1780260205. [DOI] [PubMed] [Google Scholar]
  20. Venables P. J., Smith P. R., Maini R. N. Purification and characterization of the Sjögren's syndrome A and B antigens. Clin Exp Immunol. 1983 Dec;54(3):731–738. [PMC free article] [PubMed] [Google Scholar]
  21. Zanetti M., Bigazzi P. E. Anti-idiotypic immunity and autoimmunity. I. In vitro and in vivo effects of anti-idiotypic antibodies to spontaneously occurring autoantibodies to rat thyroglobulin. Eur J Immunol. 1981 Mar;11(3):187–195. doi: 10.1002/eji.1830110306. [DOI] [PubMed] [Google Scholar]

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