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. 1988 Mar;71(3):470–474.

Defective autologous mixed leukocyte reaction in newly diagnosed type 1 diabetes mellitus.

L Räsänen 1, H Hyöty 1, M Lehto 1, O P Kallioniemi 1, J Antonen 1, T Huupponen 1, J Karjalainen 1, P Leinikki 1
PMCID: PMC1541663  PMID: 2968203

Abstract

We studied autologous mixed leukocyte reaction (AMLR) in type 1 (insulin-dependent) diabetes mellitus (IDDM) patients, their healthy siblings and healthy schoolchildren, Blood samples from the patients were drawn within 1 week after hospitalization and 2 months later. AMLR was significantly depressed in the patients when compared to healthy siblings or other healthy controls. In addition, the mean AMLR responsiveness of the healthy control group exceeded that of healthy siblings. The production of IL-2 in AMLR was impaired in the patient group and the defective AMLR could be restored by addition of exogenous IL-2 in 7-10 patient cultures. However, in 3-10 patients addition of IL-2 induced no enhancement of proliferation. While the patients in general had raised levels of activated T lymphocytes these three patients had higher numbers of activated T cells than other patients. Defective AMLR and presence of activated T cells may be related and may play a role in the pathogenesis of IDDM.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asantila T., Toivanen P. Potentation by fluorodeoxyuridine of 125I-deoxyuridine uptake by human and chicken lymphocytes in the quantitation of mitogenic response. J Immunol Methods. 1974 Dec;6(1-2):73–82. doi: 10.1016/0022-1759(74)90091-x. [DOI] [PubMed] [Google Scholar]
  2. Chandy K. G., Charles A. M., Kershnar A., Buckingham B., Waldeck N., Gupta S. Autologous mixed lymphocyte reaction in man: XV. Cellular and molecular basis of deficient autologous mixed lymphocyte response in insulin-dependent diabetes mellitus. J Clin Immunol. 1984 Nov;4(6):424–428. doi: 10.1007/BF00916571. [DOI] [PubMed] [Google Scholar]
  3. Dirienzo W., Bagnasco M., Caria M., Chimini G., Ciprandi G., Ferrini S., Canonica G. W. Activation of T lymphocytes and autologous mixed lymphocyte reaction in allergic patients. Ann Allergy. 1984 Sep;53(3):272–277. [PubMed] [Google Scholar]
  4. Gearing A. J., Johnstone A. P., Thorpe R. Production and assay of the interleukins. J Immunol Methods. 1985 Oct 24;83(1):1–27. doi: 10.1016/0022-1759(85)90053-5. [DOI] [PubMed] [Google Scholar]
  5. Gupta S. Autologous mixed lymphocyte reaction in health and disease states in man. Vox Sang. 1983;44(5):265–288. doi: 10.1111/j.1423-0410.1983.tb04483.x. [DOI] [PubMed] [Google Scholar]
  6. Gupta S., Chandy G., Thornton M., Goldberg M. Autologous mixed lymphocyte reaction in man. XIII. Characterization of the T-T autologous mixed lymphocyte reaction. J Clin Immunol. 1985 May;5(3):187–194. doi: 10.1007/BF00915510. [DOI] [PubMed] [Google Scholar]
  7. Gupta S., Fikrig S., Orti E. Autologous mixed lymphocyte reaction in man. VI. Deficiency of autologous mixed lymphocyte reaction in type I (insulin-dependent) diabetes mellitus. J Clin Lab Immunol. 1983 Jun;11(2):59–62. [PubMed] [Google Scholar]
  8. Hayward A. R., Herberger M. Culture and phenotype of activated T-cells from patients with type I diabetes mellitus. Diabetes. 1984 Apr;33(4):319–323. doi: 10.2337/diab.33.4.319. [DOI] [PubMed] [Google Scholar]
  9. Ilonen J., Lagerstedt A., Koskimies S., Reunanen M. HLA-Dw1 and BfF as protective markers in multiple sclerosis. J Neuroimmunol. 1983 Dec;5(3):283–288. doi: 10.1016/0165-5728(83)90048-6. [DOI] [PubMed] [Google Scholar]
  10. Jackson R. A., Morris M. A., Haynes B. F., Eisenbarth G. S. Increased circulating Ia-antigen-bearing T cells in type I diabetes mellitus. N Engl J Med. 1982 Apr 1;306(13):785–788. doi: 10.1056/NEJM198204013061305. [DOI] [PubMed] [Google Scholar]
  11. Kaye W. A., Adri M. N., Soeldner J. S., Rabinowe S. L., Kaldany A., Kahn C. R., Bistrian B., Srikanta S., Ganda O. P., Eisenbarth G. S. Acquired defect in interleukin-2 production in patients with type I diabetes mellitus. N Engl J Med. 1986 Oct 9;315(15):920–924. doi: 10.1056/NEJM198610093151502. [DOI] [PubMed] [Google Scholar]
  12. Koide J. Functional property of Ia-positive T cells in peripheral blood from patients with systemic lupus erythematosus. Scand J Immunol. 1985 Nov;22(5):577–584. doi: 10.1111/j.1365-3083.1985.tb01917.x. [DOI] [PubMed] [Google Scholar]
  13. Mustonen A., Knip M., Akerblom H. K. An association between complement-fixing cytoplasmic islet cell antibodies and endogenous insulin secretion in children with insulin-dependent diabetes mellitus. Diabetes. 1983 Aug;32(8):743–747. doi: 10.2337/diab.32.8.743. [DOI] [PubMed] [Google Scholar]
  14. Scudeletti M., Indiveri F., Pierri I., Picciotto A., Ferrone S. T cells from patients with chronic liver diseases: abnormalities in PHA-induced expression of HLA class II antigens and in autologous mixed-lymphocyte reactions. Cell Immunol. 1986 Oct 1;102(1):227–233. doi: 10.1016/0008-8749(86)90341-2. [DOI] [PubMed] [Google Scholar]
  15. Smolen J. S., Chused T. M., Novotny E. A., Steinberg A. D. The human autologous mixed lymphocyte reaction. III. Immune circuits. J Immunol. 1982 Sep;129(3):1050–1053. [PubMed] [Google Scholar]
  16. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]
  17. Zier K. S., Leo M. M., Spielman R. S., Baker L. Decreased synthesis of interleukin-2 (IL-2) in insulin-dependent diabetes mellitus. Diabetes. 1984 Jun;33(6):552–555. doi: 10.2337/diab.33.6.552. [DOI] [PubMed] [Google Scholar]

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