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. 1988 Mar;71(3):508–516.

Production of heavy-chain class-switch variants of human monoclonal antibody by recombinant DNA technology.

S Komori 1, N Yamasaki 1, M Shigeta 1, S Isojima 1, T Watanabe 1
PMCID: PMC1541674  PMID: 3133144

Abstract

We have previously established a human-mouse heterohybridoma (H6-3C4), which produced a human sperm-immobilizing antibody (mu, lambda of human type). The human rearranged immunoglobulin mu-chain and lambda-chain genes were cloned from the hybridoma H6-3C4. The cloned V region of the heavy chain (VH) gene was ligated to human immunoglobulin gamma 1-heavy chain constant region (C gamma 1) genes. This resulted in the heavy-chain class-switch from mu-chain to gamma 1-chain of H6-3C4 antibody. The class-switched heavy-chain gene as well as the cloned lambda-chain gene were introduced into mouse myeloma cell line X63Ag8.653 by protoplast fusion and electroporation. The stable transformants produced the human IgG monoclonal antibody, which fully retained specificity to human sperm cells and sperm-immobilizing activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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