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. 1988 Mar;71(3):377–382.

Cell-mediated immune responses to Plasmodium falciparum antigens in adult Gambians.

E M Riley 1, S Jepsen 1, G Andersson 1, L N Otoo 1, B M Greenwood 1
PMCID: PMC1541679  PMID: 3133141

Abstract

Peripheral blood mononuclear cells from clinically immune Gambian adults were assayed for in vitro proliferation in response to crude and partially purified Plasmodium falciparum antigens. Lymphoproliferative responses to malaria antigens, lectin mitogens and Candida albicans were compared with those of control donors with no previous exposure to malaria. Cells of malaria-immune individuals were significantly more responsive to conconavalin A, and less responsive to phytohaemagglutinin, than cells from the control donors in both non-immune human serum and autologous serum. Cells from a proportion of immune donors proliferated in response to soluble malaria antigens but a substantial minority did not. Young adults and women were over-represented in the non-responding population. Responses to soluble malaria antigens were depressed in autologous serum compared with normal human serum. Both immune and control cells produced low levels of gamma-IFN when stimulated with crude P. falciparum schizont antigens. Approximately half the immune donors, and none of the controls, produced significant levels of gamma-IFN in response to purified soluble malaria antigen or malaria parasite culture supernatant. There was no direct correlation between lymphoproliferation and gamma-IFN production.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ballet J. J., Druilhe P., Vasconcelos I., Schmitt C., Agrapart M., Frommel D. Human lymphocyte responses to Plasmodium falciparum merozoite antigens. A functional assay of protective immunity? Trans R Soc Trop Med Hyg. 1985;79(4):497–499. doi: 10.1016/0035-9203(85)90076-8. [DOI] [PubMed] [Google Scholar]
  2. Bygbjerg I. C., Jepsen S., Theander T. G., Odum N. Specific proliferative response of human lymphocytes to purified soluble antigens from Plasmodium falciparum in vitro cultures and to antigens from malaria patients' sera. Clin Exp Immunol. 1985 Feb;59(2):421–426. [PMC free article] [PubMed] [Google Scholar]
  3. Bygbjerg I. C., Odum N., Theander T. G. Effect of pyrimethamine and sulphadoxine on human lymphocyte proliferation. Trans R Soc Trop Med Hyg. 1986;80(2):295–300. doi: 10.1016/0035-9203(86)90040-4. [DOI] [PubMed] [Google Scholar]
  4. Druilhe P., Pradier O., Marc J. P., Miltgen F., Mazier D., Parent G. Levels of antibodies to Plasmodium falciparum sporozoite surface antigens reflect malaria transmission rates and are persistent in the absence of reinfection. Infect Immun. 1986 Aug;53(2):393–397. doi: 10.1128/iai.53.2.393-397.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Greenwood B. M., Bradley-Moore A. M., Bryceson A. D., Palit A. Immunosuppression in children with malaria. Lancet. 1972 Jan 22;1(7743):169–172. doi: 10.1016/s0140-6736(72)90569-7. [DOI] [PubMed] [Google Scholar]
  6. Jayawardena A. N., Murphy D. B., Janeway C. A., Gershon R. K. T cell-mediated immunity in malaria. I. The Ly phenotype of T cells mediating resistance to Plasmodium yoelii. J Immunol. 1982 Jul;129(1):377–381. [PubMed] [Google Scholar]
  7. Jayawardena A. N., Waksman B. H., Eardley D. D. Activation of distinct helper and suppressor T cells in experimental trypanosomiasis. J Immunol. 1978 Aug;121(2):622–628. [PubMed] [Google Scholar]
  8. Jepsen S., Axelsen N. H. Antigens and antibodies in Plasmodium falciparum malaria studied by immunoelectrophoretic methods. Acta Pathol Microbiol Scand C. 1980 Oct;88(5):263–270. doi: 10.1111/j.1699-0463.1980.tb00104.x. [DOI] [PubMed] [Google Scholar]
  9. Jepsen S., Hindersson P., Axelsen N. H. Computer controlled affinity chromatographical purification of soluble Plasmodium falciparum antigens from supernatants of in vitro cultures. Trans R Soc Trop Med Hyg. 1986;80(4):534–536. doi: 10.1016/0035-9203(86)90133-1. [DOI] [PubMed] [Google Scholar]
  10. Khansari N., Segre M., Segre D. Immunosuppression in murine malaria: a soluble immunosuppressive factor derived from Plasmodium berghei-infected blood. J Immunol. 1981 Nov;127(5):1889–1893. [PubMed] [Google Scholar]
  11. Ockenhouse C. F., Schulman S., Shear H. L. Induction of crisis forms in the human malaria parasite Plasmodium falciparum by gamma-interferon-activated, monocyte-derived macrophages. J Immunol. 1984 Sep;133(3):1601–1608. [PubMed] [Google Scholar]
  12. Perlmann H., Berzins K., Wahlgren M., Carlsson J., Björkman A., Patarroyo M. E., Perlmann P. Antibodies in malarial sera to parasite antigens in the membrane of erythrocytes infected with early asexual stages of Plasmodium falciparum. J Exp Med. 1984 Jun 1;159(6):1686–1704. doi: 10.1084/jem.159.6.1686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Theander T. G., Bygbjerg I. C., Andersen B. J., Jepsen S., Kharazmi A., Odum N. Suppression of parasite-specific response in Plasmodium falciparum malaria. A longitudinal study of blood mononuclear cell proliferation and subset composition. Scand J Immunol. 1986 Jul;24(1):73–81. doi: 10.1111/j.1365-3083.1986.tb02071.x. [DOI] [PubMed] [Google Scholar]
  14. Theander T. G., Bygbjerg I. C., Jepsen S., Svenson M., Kharazmi A., Larsen P. B., Bendtzen K. Proliferation induced by Plasmodium falciparum antigen and interleukin-2 production by lymphocytes isolated from malaria-immune individuals. Infect Immun. 1986 Jul;53(1):221–225. doi: 10.1128/iai.53.1.221-225.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Troye-Blomberg M., Andersson G., Stoczkowska M., Shabo R., Romero P., Patarroyo M. E., Wigzell H., Perlmann P. Production of IL 2 and IFN-gamma by T cells from malaria patients in response to Plasmodium falciparum or erythrocyte antigens in vitro. J Immunol. 1985 Nov;135(5):3498–3504. [PubMed] [Google Scholar]
  16. Troye-Blomberg M., Perlmann H., Patarroyo M. E., Perlmann P. Regulation of the immune response in Plasmodium falciparum malaria. II. Antigen specific proliferative responses in vitro. Clin Exp Immunol. 1983 Aug;53(2):345–353. [PMC free article] [PubMed] [Google Scholar]
  17. Troye-Blomberg M., Romero P., Patarroyo M. E., Björkman A., Perlmann P. Regulation of the immune response in Plasmodium falciparum malaria. III. Proliferative response to antigen in vitro and subset composition of T cells from patients with acute infection or from immune donors. Clin Exp Immunol. 1984 Nov;58(2):380–387. [PMC free article] [PubMed] [Google Scholar]
  18. Wilson R. J., McGregor I. A., Hall P., Williams K., Bartholomew R. Antigens associated with Plasmodium falciparum infections in man. Lancet. 1969 Jul 26;2(7613):201–205. doi: 10.1016/s0140-6736(69)91437-8. [DOI] [PubMed] [Google Scholar]
  19. Wyler D. J., Oppenheim J. J. Lymphocyte transformation in human Plasmodium falciparum malaria. J Immunol. 1974 Aug;113(2):449–454. [PubMed] [Google Scholar]

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