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. 1988 Oct;74(1):75–79.

HLA-D region antigens on isolated human colonic epithelial cells: enhanced expression in inflammatory bowel disease and in vitro induction by different stimuli.

F Pallone 1, S Fais 1, M R Capobianchi 1
PMCID: PMC1541709  PMID: 3146453

Abstract

Colonic epithelial cells (CEC) were isolated from actively inflamed mucosa of IBD patients and checked for HLA-DR, HLA-DP, and HLA-DQ. Half of the freshly isolated CEC from IBD tissue expressed DR, and one third were positive for DP and DQ. Normal human CEC were then cultured for 24 h and their expression of these markers in response to different types of in vitro stimulation was investigated. A significant increase in the expression of DR, DP and DQ was observed in response to the nonspecific mitogen phytohaemagglutinin (PHA), the lymphokine gamma-interferon (gamma-IFN) and the epidermal growth factor (EGF). The enhancement of DR expression was more marked than that of DP and DQ. The effect of gamma-IFN was more rapid and significantly more marked than that of either PHA and EGF for all three antigens. EGF appeared to be more potent than PHA in enhancing the expression of DP and DQ. The data from this study indicate that HLA-D region antigens can be induced on human CEC by different types of stimuli and provide further evidence that the expression of these markers in the colonic epithelium is a normal event the magnitude of which can increase under various circumstances. The data also suggest that the increased expression of HLA-D region antigens by IBD CEC occurs as a result of different mechanisms, and that this expression is an indicator of the active participation of the colonic epithelium to the mucosal inflammatory response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barclay A. N., Mason D. W. Induction of Ia antigen in rat epidermal cells and gut epithelium by immunological stimuli. J Exp Med. 1982 Dec 1;156(6):1665–1676. doi: 10.1084/jem.156.6.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cerf-Bensussan N., Quaroni A., Kurnick J. T., Bhan A. K. Intraepithelial lymphocytes modulate Ia expression by intestinal epithelial cells. J Immunol. 1984 May;132(5):2244–2252. [PubMed] [Google Scholar]
  3. Chopra D. P., Siddiqui K. M., Cooney R. A. Effects of insulin, transferrin, cholera toxin, and epidermal growth factor on growth and morphology of human fetal normal colon epithelial cells. Gastroenterology. 1987 Apr;92(4):891–904. doi: 10.1016/0016-5085(87)90962-0. [DOI] [PubMed] [Google Scholar]
  4. Daar A. S., Fabre J. W. The membrane antigens of human colorectal cancer cells: demonstration with monoclonal antibodies of heterogeneity within and between tumours and of anomalous expression of HLA-DR. Eur J Cancer Clin Oncol. 1983 Feb;19(2):209–220. doi: 10.1016/0277-5379(83)90419-4. [DOI] [PubMed] [Google Scholar]
  5. Geppert T. D., Lipsky P. E. Antigen presentation by interferon-gamma-treated endothelial cells and fibroblasts: differential ability to function as antigen-presenting cells despite comparable Ia expression. J Immunol. 1985 Dec;135(6):3750–3762. [PubMed] [Google Scholar]
  6. Ghosh A. K., Moore M., Street A. J., Howat J. M., Schofield P. F. Expression of HLA-D sub-region products on human colorectal carcinoma. Int J Cancer. 1986 Oct 15;38(4):459–464. doi: 10.1002/ijc.2910380402. [DOI] [PubMed] [Google Scholar]
  7. Hirata I., Austin L. L., Blackwell W. H., Weber J. R., Dobbins W. O., 3rd Immunoelectron microscopic localization of HLA-DR antigen in control small intestine and colon and in inflammatory bowel disease. Dig Dis Sci. 1986 Dec;31(12):1317–1330. doi: 10.1007/BF01299810. [DOI] [PubMed] [Google Scholar]
  8. Houghton A. N., Thomson T. M., Gross D., Oettgen H. F., Old L. J. Surface antigens of melanoma and melanocytes. Specificity of induction of Ia antigens by human gamma-interferon. J Exp Med. 1984 Jul 1;160(1):255–269. doi: 10.1084/jem.160.1.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mayer L., Shlien R. Evidence for function of Ia molecules on gut epithelial cells in man. J Exp Med. 1987 Nov 1;166(5):1471–1483. doi: 10.1084/jem.166.5.1471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mayrhofer G., Pugh C. W., Barclay A. N. The distribution, ontogeny and origin in the rat of Ia-positive cells with dendritic morphology and of Ia antigen in epithelia, with special reference to the intestine. Eur J Immunol. 1983 Feb;13(2):112–122. doi: 10.1002/eji.1830130206. [DOI] [PubMed] [Google Scholar]
  11. Natali P. G., De Martino C., Quaranta V., Nicotra M. R., Frezza F., Pellegrino M. A., Ferrone S. Expression of Ia-like antigens in normal human nonlymphoid tissues. Transplantation. 1981 Jan;31(1):75–78. doi: 10.1097/00007890-198101000-00017. [DOI] [PubMed] [Google Scholar]
  12. Pallone F., Fais S., Squarcia O., Biancone L., Pozzilli P., Boirivant M. Activation of peripheral blood and intestinal lamina propria lymphocytes in Crohn's disease. In vivo state of activation and in vitro response to stimulation as defined by the expression of early activation antigens. Gut. 1987 Jun;28(6):745–753. doi: 10.1136/gut.28.6.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Selby W. S., Janossy G., Mason D. Y., Jewell D. P. Expression of HLA-DR antigens by colonic epithelium in inflammatory bowel disease. Clin Exp Immunol. 1983 Sep;53(3):614–618. [PMC free article] [PubMed] [Google Scholar]
  14. Shorter R. G., Spencer R. J., Hallenbeck G. A. Kinetic studies of the epithelial cells of the rectal mucosa in normal subjects and patients with ulcerative colitis. Gut. 1966 Dec;7(6):593–596. doi: 10.1136/gut.7.6.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Spencer J., Finn T., Isaacson P. G. Expression of HLA-DR antigens on epithelium associated with lymphoid tissue in the human gastrointestinal tract. Gut. 1986 Feb;27(2):153–157. doi: 10.1136/gut.27.2.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wong G. H., Clark-Lewis I., McKimm-Breschkin J. L., Schrader J. W. Interferon-gamma-like molecule induces Ia antigens on cultured mast cell progenitors. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6989–6993. doi: 10.1073/pnas.79.22.6989. [DOI] [PMC free article] [PubMed] [Google Scholar]

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