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. 1988 Sep;73(3):424–429.

Presence of an IL-3-producing suppressor T cell resistant to cyclosporin A in the peripheral blood of patients with systemic lupus erythematosus.

Alcocer-Varela 1, A Vidaller 1, L Llorente 1, D Alarcón-Segovia 1
PMCID: PMC1541749  PMID: 2974765

Abstract

We studied the effects of cyclosporin A (CYA) on the production of various lymphokines and on the suppressor functions of peripheral blood mononuclear cells (MNC) of ten untreated SLE patients. CYA was found to inhibit the production of IL-2 and of B cell stimulating factor (BSF) by both normal and SLE MNC but it did not alter the LPS-driven production of IL-1 by either of them. However, it did abrogate the spontaneous release of IL-1 by SLE monocytes. CYA strongly inhibited the production of IL-3-like activity by normal T cells, an effect noticed only on cells from 4 SLE patients, three of whom were in remission. Addition of CYA significantly increased suppressor cell function by cells from all SLE patients. T cell clones could be obtained from two of them by using CYA-conditioned medium containing IL-3. These clones were found to have strong suppressor capacity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alarcon-Segovia D., Alcocer-Varela J., Diaz-Jouanen E. The connective tissue diseases as disorders of immune regulation. Clin Rheum Dis. 1985 Dec;11(3):451–469. [PubMed] [Google Scholar]
  2. Alcocer-Varela J., Alarcón-Segovia D. Decreased production of and response to interleukin-2 by cultured lymphocytes from patients with systemic lupus erythematosus. J Clin Invest. 1982 Jun;69(6):1388–1392. doi: 10.1172/JCI110579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alcocer-Varela J., Laffon A., Alarcón-Segovia D. Defective monocyte production of, and T lymphocyte response to, interleukin-1 in the peripheral blood of patients with systemic lupus erythematosus. Clin Exp Immunol. 1984 Jan;55(1):125–132. [PMC free article] [PubMed] [Google Scholar]
  4. Bunjes D., Hardt C., Röllinghoff M., Wagner H. Cyclosporin A mediates immunosuppression of primary cytotoxic T cell responses by impairing the release of interleukin 1 and interleukin 2. Eur J Immunol. 1981 Aug;11(8):657–661. doi: 10.1002/eji.1830110812. [DOI] [PubMed] [Google Scholar]
  5. Gillis S., Ferm M. M., Ou W., Smith K. A. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978 Jun;120(6):2027–2032. [PubMed] [Google Scholar]
  6. Kupiec-Weglinski J. W., Filho M. A., Strom T. B., Tilney N. L. Sparing of suppressor cells: a critical action of cyclosporine. Transplantation. 1984 Aug;38(2):97–101. doi: 10.1097/00007890-198408000-00001. [DOI] [PubMed] [Google Scholar]
  7. Lachman L. B., Hacker M. P., Handschumacher R. E. Partial purification of human lymphocyte-activating factor (LAF) by ultrafiltration and electrophoretic techniques. J Immunol. 1977 Dec;119(6):2019–2023. [PubMed] [Google Scholar]
  8. Makover D., Freundlich B., Zurier R. B. Relapse of systemic lupus erythematosus in a patient receiving cyclosporine A. J Rheumatol. 1988 Jan;15(1):117–119. [PubMed] [Google Scholar]
  9. Martinez-Cordero E., Alcocer-Varela J., Alarcon-Segovia D. Stimulating and differentiation factors for human B lymphocytes in systemic lupus erythematosus. Clin Exp Immunol. 1986 Sep;65(3):598–604. [PMC free article] [PubMed] [Google Scholar]
  10. Mohagheghpour N., Benike C. J., Kansas G., Bieber C., Engleman E. G. Activation of antigen-specific suppressor T cells in the presence of cyclosporin requires interactions between T cells of inducer and suppressor lineage. J Clin Invest. 1983 Dec;72(6):2092–2100. doi: 10.1172/JCI111174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Palacios R. Cyclosporin A inhibits antigen- and lectin-induced but not constitutive production of interleukin 3. Eur J Immunol. 1985 Feb;15(2):204–206. doi: 10.1002/eji.1830150220. [DOI] [PubMed] [Google Scholar]
  12. Palacios R., Martinez-Maza O., De Ley M. Production of human immune interferon (Hu IFN-gamma) studied at the single cell level. Origin, evidence for spontaneous secretion and effect of cyclosporin A. Eur J Immunol. 1983 Mar;13(3):221–225. doi: 10.1002/eji.1830130308. [DOI] [PubMed] [Google Scholar]
  13. Palacios R. Production of lymphokines by circulating human T lymphocytes that express or lack receptors for interleukin 2. J Immunol. 1984 Apr;132(4):1833–1836. [PubMed] [Google Scholar]
  14. Ruiz-Arguelles A., Alarcón-Segovia D., Llorente L., Del Giudice-Knipping J. A. Heterogeneity of the spontaneously expanded and mitogen-induced generation of suppressor cell function of T cells on B cells in systemic lupus erythematosus. Arthritis Rheum. 1980 Sep;23(9):1004–1009. doi: 10.1002/art.1780230907. [DOI] [PubMed] [Google Scholar]
  15. Sakane T., Takada S., Suzuki N., Tsuchida T., Murakawa Y., Ueda Y. Deficiencies in suppressor T cell activity seen in patients with active systemic lupus erythematosus are due to the dilution of normally functioning suppressor T cells by nonsuppressor T cells. J Immunol. 1986 Dec 15;137(12):3809–3813. [PubMed] [Google Scholar]
  16. Shevach E. M. The effects of cyclosporin A on the immune system. Annu Rev Immunol. 1985;3:397–423. doi: 10.1146/annurev.iy.03.040185.002145. [DOI] [PubMed] [Google Scholar]
  17. Smith K. A., Lachman L. B., Oppenheim J. J., Favata M. F. The functional relationship of the interleukins. J Exp Med. 1980 Jun 1;151(6):1551–1556. doi: 10.1084/jem.151.6.1551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]

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