Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1988 Sep;73(3):355–359.

Specific antibody synthesis in vitro. IV. The correlation of in vitro and in vivo antibody response to influenza vaccine in rhesus monkeys.

W B Ershler 1, C L Coe 1, S Gravenstein 1, K T Schultz 1, R G Klopp 1
PMCID: PMC1541779  PMID: 3208447

Abstract

Fourteen female monkeys (Macaca mulatta) received a trivalent influenza vaccine and antibody response was determined by a change in plasma antibody content (ELISA) before and after vaccine. Lymphocyte cultures were also established from these monkeys and the level of antibody response did not correlate with mitogen-induced lymphocyte blastogenesis or natural killer cell function. In vitro anti-influenza antibody synthesis, however, was found to correlate well with the in vivo response. That is, monkeys who were non-responders, as determined by lack of change in plasma antibody content, were also non-responders in vitro. Accordingly, we believe that vaccine response is not necessarily a measure of immune competence but its measurement may, none the less, have clinical utility. The excellent correlation of in vivo and in vitro response provides predictive value for the in vitro test. Furthermore, because the correlation is good, the in vitro test may be useful as a tool in immunopharmacology and toxicology.

Full text

PDF
355

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ershler W. B., Moore A. L., Hacker M. P., Ninomiya J., Naylor P., Goldstein A. L. Specific antibody synthesis in vitro. II. Age-associated thymosin enhancement of antitetanus antibody synthesis. Immunopharmacology. 1984 Oct;8(2):69–77. doi: 10.1016/0162-3109(84)90045-6. [DOI] [PubMed] [Google Scholar]
  2. Ershler W. B., Moore A. L., Socinski M. A. Influenza and aging: age-related changes and the effects of thymosin on the antibody response to influenza vaccine. J Clin Immunol. 1984 Nov;4(6):445–454. doi: 10.1007/BF00916574. [DOI] [PubMed] [Google Scholar]
  3. Ershler W. B., Moore A. L., Socinski M. A. Specific antibody synthesis in vitro. III. Correlation of in vivo and in vitro antibody response to influenza immunization in young and old subjects. J Clin Lab Immunol. 1985 Feb;16(2):63–67. [PubMed] [Google Scholar]
  4. Fauci A. S., Dale D. C., Balow J. E. Glucocorticosteroid therapy: mechanisms of action and clinical considerations. Ann Intern Med. 1976 Mar;84(3):304–315. doi: 10.7326/0003-4819-84-3-304. [DOI] [PubMed] [Google Scholar]
  5. Gillis S., Kozak R., Durante M., Weksler M. E. Immunological studies of aging. Decreased production of and response to T cell growth factor by lymphocytes from aged humans. J Clin Invest. 1981 Apr;67(4):937–942. doi: 10.1172/JCI110143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hahn B. H., MacDermott R. P., Jacobs S. B., Pletscher L. S., Beale M. G. Immunosuppressive effects of low doses of glucocorticoids: effects on autologous and allogeneic mixed leukocyte reactions. J Immunol. 1980 Jun;124(6):2812–2817. [PubMed] [Google Scholar]
  7. Kishimoto S., Tomino S., Mitsuya H., Fujiwara H., Tsuda H. Age-related decline in the in vitro and in vivo syntheses of anti-tetanus toxoid antibody in humans. J Immunol. 1980 Nov;125(5):2347–2352. [PubMed] [Google Scholar]
  8. Levine M., Beattie B. L., McLean D. M., Corman D. Characterization of the immune response to trivalent influenza vaccine in elderly men. J Am Geriatr Soc. 1987 Jul;35(7):609–615. doi: 10.1111/j.1532-5415.1987.tb04335.x. [DOI] [PubMed] [Google Scholar]
  9. Makinodan T., Kay M. M. Age influence on the immune system. Adv Immunol. 1980;29:287–330. doi: 10.1016/s0065-2776(08)60047-4. [DOI] [PubMed] [Google Scholar]
  10. Mazaheri R., Rode H. N., Abikar K., Ing A., Nohr C., Gordon J. Dysfunction of humoral immunity in anergic surgical patients: absence of anti-tetanus IgG antibody production. J Clin Immunol. 1984 Jan;4(1):65–70. doi: 10.1007/BF00915289. [DOI] [PubMed] [Google Scholar]
  11. Miller R. A. Immunodeficiency of aging: restorative effects of phorbol ester combined with calcium ionophore. J Immunol. 1986 Aug 1;137(3):805–808. [PubMed] [Google Scholar]
  12. Moore A. L., Ershler W. B., Hacker M. P. Specific antibody synthesis in vitro. I. Technical considerations. J Immunol Methods. 1984 May 11;70(1):13–21. doi: 10.1016/0022-1759(84)90384-3. [DOI] [PubMed] [Google Scholar]
  13. Murphy B. R., Tierney E. L., Barbour B. A., Yolken R. H., Alling D. W., Holley H. P., Jr, Mayner R. E., Chanock R. M. Use of the enzyme-linked immunosorbent assay to detect serum antibody responses of volunteers who received attenuated influenza A virus vaccines. Infect Immun. 1980 Aug;29(2):342–347. doi: 10.1128/iai.29.2.342-347.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nordin A. A., Makinodan T. Humoral immunity in aging. Fed Proc. 1974 Sep;33(9):2033–2035. [PubMed] [Google Scholar]
  15. Price G. B., Makinodan T. Immunologic deficiencies in senescence. I. Characterization of intrinsic deficiencies. J Immunol. 1972 Feb;108(2):403–412. [PubMed] [Google Scholar]
  16. Stevens R. H., Saxon A. Differential synthesis of IgM and IgA anti-tetanus toxoid antibody in vitro following in vivo booster immunization of humans. Cell Immunol. 1979 Jun;45(1):142–150. doi: 10.1016/0008-8749(79)90369-1. [DOI] [PubMed] [Google Scholar]
  17. Stevens R. H., Saxon A. Immunoregulation in humans: control of antitetanus toxoid antibody production after booster immunization. J Clin Invest. 1978 Dec;62(6):1154–1160. doi: 10.1172/JCI109234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Virelizier J. L., Postlethwaite R., Schild G. C., Allison A. C. Antibody responses to antigenic determinants of influenza virus hemagglutinin. I. Thymus dependence of antibody formation and thymus independence of immunological memory. J Exp Med. 1974 Dec 1;140(6):1559–1570. doi: 10.1084/jem.140.6.1559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Walford R. L. Immunologic theory of aging: current status. Fed Proc. 1974 Sep;33(9):2020–2027. [PubMed] [Google Scholar]
  20. Weitzman S. A., Aisenberg A. C., Siber G. R., Smith D. H. Impaired humoral immunity in treated Hodgkin's disease. N Engl J Med. 1977 Aug 4;297(5):245–248. doi: 10.1056/NEJM197708042970504. [DOI] [PubMed] [Google Scholar]
  21. Weksler M. C., Innes J. D., Goldstein G. Immunological studies of aging. IV. The contribution of thymic involution to the immune deficiencies of aging mice and reversal with thymopoietin32-36. J Exp Med. 1978 Oct 1;148(4):996–1006. doi: 10.1084/jem.148.4.996. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES