Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1988 Nov;74(2):283–288.

Autoantibodies to 200 kD polypeptide(s) of the nuclear envelope: a new serologic marker of primary biliary cirrhosis.

K Lassoued 1, M N Guilly 1, C Andre 1, M Paintrand 1, D Dhumeaux 1, F Danon 1, J C Brouet 1, J C Courvalin 1
PMCID: PMC1541802  PMID: 3066540

Abstract

Fifty sera which gave ring-like nuclear staining in immunofluorescence on rat liver tissue sections were characterized. Using immunoprecipitation and Western blotting we showed that 10 of the sera contained antibodies to 200 kD polypeptide(s) of nuclear envelope. Clinical and biological data were available for nine of the patients. Strikingly, all of these patients suffered from primary biliary cirrhosis with eight of them having anti-mitochondrial antibodies. As no control serum displayed such a reactivity, anti-200 kD polypeptide(s) antibodies can be considered as a new marker specific of a subset of primary biliary cirrhosis, being present even when anti-mitochondrial antibodies are absent. The exact identity of the target remains to be established, since several polypeptides of similar molecular weight have been reported to belong to the nuclear envelope.

Full text

PDF
283

Images in this article

285Fig. 2
on p.285
285Fig. 3
on p.285
286Fig. 4
on p.286
286Fig. 5
on p.286

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baum H., Berg P. A. The complex nature of mitochondrial antibodies and their relation to primary biliary cirrhosis. Semin Liver Dis. 1981 Nov;1(4):309–321. doi: 10.1055/s-2008-1040734. [DOI] [PubMed] [Google Scholar]
  2. Berrios M., Blobel G., Fisher P. A. Characterization of an ATPase/dATPase activity associated with the Drosophila nuclear matrix-pore complex-lamina fraction. Identification of the putative enzyme polypeptide by direct ultraviolet photoaffinity labeling. J Biol Chem. 1983 Apr 10;258(7):4548–4555. [PubMed] [Google Scholar]
  3. Berrios M., Filson A. J., Blobel G., Fisher P. A. A 174-kilodalton ATPase/dATPase polypeptide and a glycoprotein of apparently identical molecular weight are common but distinct components of higher eukaryotic nuclear structural protein subfractions. J Biol Chem. 1983 Nov 10;258(21):13384–13390. [PubMed] [Google Scholar]
  4. Berrios M., Fisher P. A. A myosin heavy-chain-like polypeptide is associated with the nuclear envelope in higher eukaryotic cells. J Cell Biol. 1986 Sep;103(3):711–724. doi: 10.1083/jcb.103.3.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Blobel G., Potter V. R. Nuclei from rat liver: isolation method that combines purity with high yield. Science. 1966 Dec 30;154(3757):1662–1665. doi: 10.1126/science.154.3757.1662. [DOI] [PubMed] [Google Scholar]
  6. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  7. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  8. Christensen E., Crowe J., Doniach D., Popper H., Ranek L., Rodés J., Tygstrup N., Williams R. Clinical pattern and course of disease in primary biliary cirrhosis based on an analysis of 236 patients. Gastroenterology. 1980 Feb;78(2):236–246. [PubMed] [Google Scholar]
  9. Gerace L., Comeau C., Benson M. Organization and modulation of nuclear lamina structure. J Cell Sci Suppl. 1984;1:137–160. doi: 10.1242/jcs.1984.supplement_1.10. [DOI] [PubMed] [Google Scholar]
  10. Gerace L., Ottaviano Y., Kondor-Koch C. Identification of a major polypeptide of the nuclear pore complex. J Cell Biol. 1982 Dec;95(3):826–837. doi: 10.1083/jcb.95.3.826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  12. Guilly M. N., Danon F., Brouet J. C., Bornens M., Courvalin J. C. Autoantibodies to nuclear lamin B in a patient with thrombopenia. Eur J Cell Biol. 1987 Apr;43(2):266–272. [PubMed] [Google Scholar]
  13. Kaplan M. M. Primary biliary cirrhosis. N Engl J Med. 1987 Feb 26;316(9):521–528. doi: 10.1056/NEJM198702263160907. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Lassoued K., Guilly M. N., Danon F., Andre C., Dhumeaux D., Clauvel J. P., Brouet J. C., Seligmann M., Courvalin J. C. Antinuclear autoantibodies specific for lamins. Characterization and clinical significance. Ann Intern Med. 1988 Jun;108(6):829–833. doi: 10.7326/0003-4819-108-6-829. [DOI] [PubMed] [Google Scholar]
  16. McKeon F. D., Tuffanelli D. L., Fukuyama K., Kirschner M. W. Autoimmune response directed against conserved determinants of nuclear envelope proteins in a patient with linear scleroderma. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4374–4378. doi: 10.1073/pnas.80.14.4374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reeves W. H., Chaudhary N., Salerno A., Blobel G. Lamin B autoantibodies in sera of certain patients with systemic lupus erythematosus. J Exp Med. 1987 Mar 1;165(3):750–762. doi: 10.1084/jem.165.3.750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ruffatti A., Arslan P., Floreani A., De Silvestro G., Calligaro A., Naccarato R., Todesco S. Nuclear membrane-staining antinuclear antibody in patients with primary biliary cirrhosis. J Clin Immunol. 1985 Sep;5(5):357–361. doi: 10.1007/BF00918255. [DOI] [PubMed] [Google Scholar]
  19. Sherlock S., Scheuer P. J. The presentation and diagnosis of 100 patients with primary biliary cirrhosis. N Engl J Med. 1973 Sep 27;289(13):674–678. doi: 10.1056/NEJM197309272891306. [DOI] [PubMed] [Google Scholar]
  20. Snow C. M., Senior A., Gerace L. Monoclonal antibodies identify a group of nuclear pore complex glycoproteins. J Cell Biol. 1987 May;104(5):1143–1156. doi: 10.1083/jcb.104.5.1143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tan E. M. Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine. Adv Immunol. 1982;33:167–240. doi: 10.1016/s0065-2776(08)60836-6. [DOI] [PubMed] [Google Scholar]
  22. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES