Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1989 Jan;75(1):82–86.

Mononuclear phagocyte system of human Peyer's patches: an immunohistochemical study using monoclonal antibodies.

Y R Mahida 1, S Patel 1, D P Jewell 1
PMCID: PMC1541871  PMID: 2784731

Abstract

Macrophages in sections of human terminal ileal Peyer's patches were studied using a panel of monoclonal antibodies. Germinal centre macrophages were large and strongly positive for acid phosphatase and stained with antibodies RFD1, RFD9, UCHM1 and other macrophage-specific monoclonal antibodies, but not RFD7. In the macrophages of the dome region there was heterogeneity of size and staining for acid phosphatase. The majority of the RFD1 positive cells in this region appeared to be macrophages. However, no RFD9- or RFD7-positive cells were present. By contrast, RFD7-positive but RFD9-negative macrophages were present in the lamina propria. In the interfollicular areas there were cells with a dendritic morphology which were strongly HLA-DR, HLA-DQ and RFD1-positive, but which did not stain with the other macrophage specific monoclonal antibodies or for acid phosphatase. Some macrophages and lymphocytes in the germinal centre and dome regions expressed interleukin 2 and transferrin receptors. These cells were not present in the adjacent lamina propria.

Full text

PDF
82

Images in this article

83Fig. 2
on p.83
84Fig. 3
on p.84
84Fig. 4
on p.84
84Fig. 5
on p.84
85Fig. 6
on p.85

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austyn J. M. Lymphoid dendritic cells. Immunology. 1987 Oct;62(2):161–170. [PMC free article] [PubMed] [Google Scholar]
  2. Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. doi: 10.1177/32.2.6198355. [DOI] [PubMed] [Google Scholar]
  3. Cornes J. S. Number, size, and distribution of Peyer's patches in the human small intestine: Part I The development of Peyer's patches. Gut. 1965 Jun;6(3):225–229. doi: 10.1136/gut.6.3.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dijkstra C. D., Döpp E. A., Joling P., Kraal G. The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology. 1985 Mar;54(3):589–599. [PMC free article] [PubMed] [Google Scholar]
  5. Hancock W. W., Muller W. A., Cotran R. S. Interleukin 2 receptors are expressed by alveolar macrophages during pulmonary sarcoidosis and are inducible by lymphokine treatment of normal human lung macrophages, blood monocytes, and monocyte cell lines. J Immunol. 1987 Jan 1;138(1):185–191. [PubMed] [Google Scholar]
  6. Holter W., Goldman C. K., Casabo L., Nelson D. L., Greene W. C., Waldmann T. A. Expression of functional IL 2 receptors by lipopolysaccharide and interferon-gamma stimulated human monocytes. J Immunol. 1987 May 1;138(9):2917–2922. [PubMed] [Google Scholar]
  7. Hume D. A., Robinson A. P., MacPherson G. G., Gordon S. The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs. J Exp Med. 1983 Nov 1;158(5):1522–1536. doi: 10.1084/jem.158.5.1522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MacDonald T. T., Carter P. B. Isolation and functional characteristics of adherent phagocytic cells from mouse Peyer's patches. Immunology. 1982 Apr;45(4):769–774. [PMC free article] [PubMed] [Google Scholar]
  9. Mayrhofer G., Pugh C. W., Barclay A. N. The distribution, ontogeny and origin in the rat of Ia-positive cells with dendritic morphology and of Ia antigen in epithelia, with special reference to the intestine. Eur J Immunol. 1983 Feb;13(2):112–122. doi: 10.1002/eji.1830130206. [DOI] [PubMed] [Google Scholar]
  10. Poulter L. W., Campbell D. A., Munro C., Janossy G. Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies. Scand J Immunol. 1986 Sep;24(3):351–357. doi: 10.1111/j.1365-3083.1986.tb02104.x. [DOI] [PubMed] [Google Scholar]
  11. Pugh C. W., MacPherson G. G., Steer H. W. Characterization of nonlymphoid cells derived from rat peripheral lymph. J Exp Med. 1983 Jun 1;157(6):1758–1779. doi: 10.1084/jem.157.6.1758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Selby W. S., Poulter L. W., Hobbs S., Jewell D. P., Janossy G. Heterogeneity of HLA-DR-positive histiocytes in human intestinal lamina propria: a combined histochemical and immunohistological analysis. J Clin Pathol. 1983 Apr;36(4):379–384. doi: 10.1136/jcp.36.4.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Spalding D. M., Koopman W. J., Eldridge J. H., McGhee J. R., Steinman R. M. Accessory cells in murine Peyer's patch. I. Identification and enrichment of a functional dendritic cell. J Exp Med. 1983 May 1;157(5):1646–1659. doi: 10.1084/jem.157.5.1646. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Spencer J., Finn T., Isaacson P. G. Human Peyer's patches: an immunohistochemical study. Gut. 1986 Apr;27(4):405–410. doi: 10.1136/gut.27.4.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Volkman A. Disparity in origin of mononuclear phagocyte populations. J Reticuloendothel Soc. 1976 Apr;19(4):249–268. [PubMed] [Google Scholar]
  16. Wilders M. M., Sminia T., Plesch B. E., Drexhage H. A., Weltevreden E. F., Meuwissen S. G. Large mononuclear Ia-positive veiled cells in Peyer's patches. II. Localization in rat Peyer's patches. Immunology. 1983 Mar;48(3):461–467. [PMC free article] [PubMed] [Google Scholar]
  17. Yunis E., Sherman F. E. Macrophages of the rectal lamina propria in children. Am J Clin Pathol. 1970 May;53(5):580–591. doi: 10.1093/ajcp/53.5.580. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES