Abstract
The sera of 46 patients with multiple myeloma were examined for the presence of anti-tuberculosis (TB) glycolipids antibodies. In positive sera samples, anti-polynucleotides, anti-histones, anti-cardiolipin, anti-Sm and anti-RNP activities were sought. Antibodies against three different mycobacterial glycolipids were detected in 10 of the 46 sera. Three (P429, P557, P5) showed high titres of antibodies against all three different TB glycolipids. Of the 10 reactive samples, two antibodies were purified (P429, P557). P557 reacted with various polynucleotides and other antigens tested (Sm, RNP, cardiolipin, histones). One immunoglobulin (P207) which showed high activity against Sm and RNP had no activity against TB glycolipids and was employed as a control. The cross-reactivity between mycobacterial glycolipids and the nuclear antigens was further established by bi-directional competition assays with P429 and P557. Our study shows a high incidence (22%) of anti-TB glycolipids antibodies in sera of patients with monoclonal gammopathies, some of which show anti-DNA and other anti-nuclear antigens activities. This is additional evidence for the mycobacterial-nuclear antigen cross-reactivity which may suggest a possible role of infection (e.g., tuberculosis) in autoimmune diseases.
Full text
PDF





Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- André-Schwartz J., Datta S. K., Shoenfeld Y., Isenberg D. A., Stollar B. D., Schwartz R. S. Binding of cytoskeletal proteins by monoclonal anti-DNA lupus autoantibodies. Clin Immunol Immunopathol. 1984 May;31(2):261–271. doi: 10.1016/0090-1229(84)90246-0. [DOI] [PubMed] [Google Scholar]
- Atkinson P. M., Lampman G. W., Furie B. C., Naparstek Y., Schwartz R. S., Stollar B. D., Furie B. Homology of the NH2-terminal amino acid sequences of the heavy and light chains of human monoclonal lupus autoantibodies containing the dominant 16/6 idiotype. J Clin Invest. 1985 Apr;75(4):1138–1143. doi: 10.1172/JCI111808. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Diamond B., Scharff M. D. Somatic mutation of the T15 heavy chain gives rise to an antibody with autoantibody specificity. Proc Natl Acad Sci U S A. 1984 Sep;81(18):5841–5844. doi: 10.1073/pnas.81.18.5841. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dighiero G., Guilbert B., Fermand J. P., Lymberi P., Danon F., Avrameas S. Thirty-six human monoclonal immunoglobulins with antibody activity against cytoskeleton proteins, thyroglobulin, and native DNA: immunologic studies and clinical correlations. Blood. 1983 Aug;62(2):264–270. [PubMed] [Google Scholar]
- Eilat D., Hochberg M., Pumphrey J., Rudikoff S. Monoclonal antibodies to DNA and RNA from NZB/NZW F1 mice: antigenic specificities and NH2 terminal amino acid sequences. J Immunol. 1984 Jul;133(1):489–494. [PubMed] [Google Scholar]
- Intrator L., Andre C., Chenal C., Sultan C. A monoclonal macroglobulin with antinuclear activity. J Clin Pathol. 1979 May;32(5):450–454. doi: 10.1136/jcp.32.5.450. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Isenberg D. A., Shoenfeld Y., Schwartz R. S. Multiple serologic reactions and their relationship to clinical activity in systemic lupus erythematosus. Arthritis Rheum. 1984 Feb;27(2):132–138. doi: 10.1002/art.1780270203. [DOI] [PubMed] [Google Scholar]
- Isenberg D. A., Shoenfeld Y., Walport M., Mackworth-Young C., Dudeney C., Todd-Pokropek A., Brill S., Weinberger A., Pinkas J. Detection of cross-reactive anti-DNA antibody idiotypes in the serum of systemic lupus erythematosus patients and of their relatives. Arthritis Rheum. 1985 Sep;28(9):999–1007. doi: 10.1002/art.1780280907. [DOI] [PubMed] [Google Scholar]
- Mendlovic S., Brocke S., Shoenfeld Y., Ben-Bassat M., Meshorer A., Bakimer R., Mozes E. Induction of a systemic lupus erythematosus-like disease in mice by a common human anti-DNA idiotype. Proc Natl Acad Sci U S A. 1988 Apr;85(7):2260–2264. doi: 10.1073/pnas.85.7.2260. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Michaelsen T. E., Gaarder P. I., Kornstad L. A transient monoclonal human IgG1 kappa protein with anti-thyroglobulin activity: lack of cross-idiotypy with polyclonal anti-thyroglobulin antibodies. Clin Exp Immunol. 1981 Nov;46(2):268–276. [PMC free article] [PubMed] [Google Scholar]
- Murray H. W. Transient autoimmune hemolytic anemia and pulmonary tuberculosis. N Engl J Med. 1978 Aug 31;299(9):488–488. doi: 10.1056/nejm197808312990920. [DOI] [PubMed] [Google Scholar]
- Naparstek Y., Duggan D., Schattner A., Madaio M. P., Goni F., Frangione B., Stollar B. D., Kabat E. A., Schwartz R. S. Immunochemical similarities between monoclonal antibacterial Waldenstrom's macroglobulins and monoclonal anti-DNA lupus autoantibodies. J Exp Med. 1985 Jun 1;161(6):1525–1538. doi: 10.1084/jem.161.6.1525. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pangborn M. C., McKinney J. A. Purification of serologically active phosphoinositides of Mycobacterium tuberculosis. J Lipid Res. 1966 Sep;7(5):627–633. [PubMed] [Google Scholar]
- Pick A. I., Shoenfeld Y., Frohlichmann R., Weiss H., Vana D., Schreibman S. Plasma cell dyscrasia. Analysis of 423 patients. JAMA. 1979 May 25;241(21):2275–2278. [PubMed] [Google Scholar]
- Potter M., Lieberman R. Common individual antigenic determinants in five of eight BALB-c IgA myeloma proteins that bind phosphoryl choline. J Exp Med. 1970 Oct 1;132(4):737–751. doi: 10.1084/jem.132.4.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sela O., el-Roeiy A., Isenberg D. A., Kennedy R. C., Colaco C. B., Pinkhas J., Shoenfeld Y. A common anti-DNA idiotype in sera of patients with active pulmonary tuberculosis. Arthritis Rheum. 1987 Jan;30(1):50–56. doi: 10.1002/art.1780300107. [DOI] [PubMed] [Google Scholar]
- Seligmann M., Brouet J. C. Antibody activity of human myeloma globulins. Semin Hematol. 1973 Apr;10(2):163–177. [PubMed] [Google Scholar]
- Seligmann M., Meshaka G., Hurez D., Mihaesco C. Les globulines myélomateuses sont-elles anormales. Nouv Rev Fr Hematol. 1967 Sep-Oct;7(5):765–778. [PubMed] [Google Scholar]
- Seligmann M., Sassy C., Chevalier A. A human IgG myeloma protein with anti- 2 macroglobulin antibody activity. J Immunol. 1973 Jan;110(1):85–90. [PubMed] [Google Scholar]
- Shoenfeld Y., Ben-Yehuda O., Napartstek Y., Wilner Y., Frolichman R., Schattner A., Lavie G., Joshua H., Pinkhas J., Kennedy R. C. The detection of a common idiotype of anti-DNA antibodies in the sera of patients with monoclonal gammopathies. J Clin Immunol. 1986 May;6(3):194–204. doi: 10.1007/BF00918699. [DOI] [PubMed] [Google Scholar]
- Shoenfeld Y., Hsu-Lin S. C., Gabriels J. E., Silberstein L. E., Furie B. C., Furie B., Stollar B. D., Schwartz R. S. Production of autoantibodies by human-human hybridomas. J Clin Invest. 1982 Jul;70(1):205–208. doi: 10.1172/JCI110595. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shoenfeld Y., Isenberg D. A. Mycobacteria and autoimmunity. Immunol Today. 1988 Jun;9(6):178–182. doi: 10.1016/0167-5699(88)91294-7. [DOI] [PubMed] [Google Scholar]
- Shoenfeld Y., Rauch J., Massicotte H., Datta S. K., André-Schwartz J., Stollar B. D., Schwartz R. S. Polyspecificity of monoclonal lupus autoantibodies produced by human-human hybridomas. N Engl J Med. 1983 Feb 24;308(8):414–420. doi: 10.1056/NEJM198302243080802. [DOI] [PubMed] [Google Scholar]
- Shoenfeld Y., Schwartz R. S. Immunologic and genetic factors in autoimmune diseases. N Engl J Med. 1984 Oct 18;311(16):1019–1029. doi: 10.1056/NEJM198410183111605. [DOI] [PubMed] [Google Scholar]
- Shoenfeld Y., Segol G., Segol O., Neary B., Klajman A., Stollar B. D., Isenberg D. A. Detection of antibodies to total histones and their subfractions in systemic lupus erythematosus patients and their asymptomatic relatives. Arthritis Rheum. 1987 Feb;30(2):169–175. doi: 10.1002/art.1780300207. [DOI] [PubMed] [Google Scholar]
- Shoenfeld Y., el-Roeiy A., Ben-Yehuda O., Pick A. I. Detection of anti-histone activity in sera of patients with monoclonal gammopathies. Clin Immunol Immunopathol. 1987 Feb;42(2):250–258. doi: 10.1016/0090-1229(87)90012-2. [DOI] [PubMed] [Google Scholar]
- Teplizki H., Buskila D., Alkan M., Coates A. R., Baumgarten A., Shoenfeld Y. ELISA measurement of antibody titer to purified protein derivative and mycobacteria-derived phosphoglycolipids: tool for diagnosing active pulmonary tuberculosis. Isr J Med Sci. 1987 Nov;23(11):1121–1124. [PubMed] [Google Scholar]
- Teplizki H., Buskila D., Argov S., Isenberg D., Coates A. R., Sukenik S., Horowitz J., Shoenfeld Y. Low serum antimycobacterial glycolipid antibody titers in the sera of patients with systemic lupus erythematosus associated with central nervous system involvement. J Rheumatol. 1987 Jun;14(3):507–511. [PubMed] [Google Scholar]
- Zurgil N., Shafrir S., Slor H., Shoenfeld Y. Identification of anti-Sm and anti-RNP autoantibodies by immunoblotting and immunoassay. Isr J Med Sci. 1988 Dec;24(12):751–754. [PubMed] [Google Scholar]