Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1989 Jun;76(3):361–365.

Humoral immune response to individual Yersinia enterocolitica antigens in patients with and without reactive arthritis.

A Grönberg 1, A Frydén 1, E Kihlström 1
PMCID: PMC1541884  PMID: 2752593

Abstract

The IgG, IgA and IgM antibody responses to individual Yersinia enterocolitica antigens were analysed by immunoblotting in 10 patients with reactive arthritis and 11 patients with enterocolitis caused by Y. enterocolitica serotype 0:3. The IgG antibody response was directed against partly different antigens compared with IgA. The expression of these antigens varied with bacterial culture conditions. The IgG and IgM antibody responses in the early stages of infection (less than or equal to 3 weeks) were directed against a large number of antigens. The IgA response was more selective, with antibodies against 240, 48 and 34.5 kD antigens dominating. IgA antibodies against eight antigens ranging in molecular weight from 52.5 to 105 kD were seen exclusively among arthritic patients. When following the IgA antibody response in the two groups of patients for up to 993 days, antibodies tended to persist to selective antigens. However, these antigens varied to some extent between patients, but the persistence was seen both among arthritic and non-arthritic patients. Persistent antibodies to 240, 48 and 34.5 kD antigens were most common. IgA antibodies to a 112 kD antigen were more prevalent and persisted longer in patients with arthritis compared to patients with uncomplicated infection.

Full text

PDF
361

Images in this article

363Fig. 1
on p.363
364Fig. 2
on p.364

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bengtsson A., Ahlstrand C., Lindström F. D., Kihlström E. Bacteriological findings in 25 patients with Reiter's syndrome (reactive arthritis). Scand J Rheumatol. 1983;12(2):157–160. doi: 10.3109/03009748309102903. [DOI] [PubMed] [Google Scholar]
  2. Bengtsson A., Holmlund G., Kihlström E., Lindblom B. Lymphocyte stimulation in Reiter's syndrome. A comparative study between patients and normal controls with special reference to HLA-B27. Scand J Rheumatol. 1985;14(4):381–385. doi: 10.3109/03009748509102042. [DOI] [PubMed] [Google Scholar]
  3. Blake M. S., Johnston K. H., Russell-Jones G. J., Gotschlich E. C. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal Biochem. 1984 Jan;136(1):175–179. doi: 10.1016/0003-2697(84)90320-8. [DOI] [PubMed] [Google Scholar]
  4. Bölin I., Portnoy D. A., Wolf-Watz H. Expression of the temperature-inducible outer membrane proteins of yersiniae. Infect Immun. 1985 Apr;48(1):234–240. doi: 10.1128/iai.48.1.234-240.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gemski P., Lazere J. R., Casey T. Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica. Infect Immun. 1980 Feb;27(2):682–685. doi: 10.1128/iai.27.2.682-685.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Granfors K., Toivanen A. IgA-anti-yersinia antibodies in yersinia triggered reactive arthritis. Ann Rheum Dis. 1986 Jul;45(7):561–565. doi: 10.1136/ard.45.7.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gripenberg M. Common serological features in rheumatoid arthritis and yersinia arthritis. Demonstration of rheumatoid factors and antibodies against ssDNA and Yersinia enterocolitica Lipopolysaccharide by ELISA. Scand J Rheumatol. 1981;10(2):85–91. doi: 10.3109/03009748109095278. [DOI] [PubMed] [Google Scholar]
  8. Grönberg A., Kihlström E. Structural variations and growth potential of Yersinia enterocolitica under different culture conditions. APMIS. 1989 Mar;97(3):227–235. [PubMed] [Google Scholar]
  9. Inman R. D. The interplay of microbe and MHC in the pathogenesis of Reiter's syndrome. Clin Exp Rheumatol. 1986 Jan-Mar;4(1):75–82. [PubMed] [Google Scholar]
  10. Keat A. Reiter's syndrome and reactive arthritis in perspective. N Engl J Med. 1983 Dec 29;309(26):1606–1615. doi: 10.1056/NEJM198312293092604. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Mattila P. S., Valtonen V., Tuori M. R., Mäkelä O. Antibody responses in arthritic and uncomplicated Yersinia enterocolitica infections. J Clin Immunol. 1985 Nov;5(6):404–411. doi: 10.1007/BF00915338. [DOI] [PubMed] [Google Scholar]
  13. Mazza G., Karu A. E., Kingsbury D. T. Immune response to plasmid- and chromosome-encoded Yersinia antigens. Infect Immun. 1985 Jun;48(3):676–685. doi: 10.1128/iai.48.3.676-685.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Skurnik M. Expression of antigens encoded by the virulence plasmid of Yersinia enterocolitica under different growth conditions. Infect Immun. 1985 Jan;47(1):183–190. doi: 10.1128/iai.47.1.183-190.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ståhlberg T. H., Granfors K., Toivanen A. Immunoblot analysis of human IgM, IgG and IgA responses to plasmid-encoded antigens of Yersinia enterocolitica serovar O3. J Med Microbiol. 1987 Sep;24(2):157–163. doi: 10.1099/00222615-24-2-157. [DOI] [PubMed] [Google Scholar]
  16. Ståhlberg T. H., Tertti R., Wolf-Watz H., Granfors K., Toivanen A. Antibody response in Yersinia pseudotuberculosis III infection: analysis of an outbreak. J Infect Dis. 1987 Aug;156(2):388–391. doi: 10.1093/infdis/156.2.388. [DOI] [PubMed] [Google Scholar]
  17. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Zaleska M., Lounatmaa K., Nurminen M., Wahlström E., Mäkelä P. H. A novel virulence-associated cell surface structure composed of 47-kd protein subunits in Yersinia enterocolitica. EMBO J. 1985 Apr;4(4):1013–1018. doi: 10.1002/j.1460-2075.1985.tb03732.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES