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. 1989 Jun;76(3):434–439.

Histamine release from basophils in cystic fibrosis.

W Schönfeld 1, A Saak 1, G Steinkamp 1, H Van der Hardt 1, W König 1
PMCID: PMC1541905  PMID: 2473860

Abstract

We determined the histamine release from basophils in patients suffering from cystic fibrosis (CF) (median 17.2 years of age) and compared the data with an age-matched group of healthy donors. No significant differences in the basophil counts were determined between the CF and control groups. However, the absolute histamine content per basophil was elevated in the CF group (2.6 +/- 0.4 pg/basophil versus 1.4 +/- 0.2, mean +/- s.e.m., n = 15/10, P less than 0.004). Stimulation of basophils with the Ca ionophore (7.5 microM) and anti-IgE (10(-2) of a stock preparation) leads to a significantly higher release of histamine per basophil in CF patients as compared to healthy donors (Ca ionophore: 1.6 +/- 0.2 versus 0.9 +/- 0.2, mean +/- s.e.m., P less than 0.008; anti-IgE: 0.45 +/- 0.007 versus 0.28 +/- 0.04, P less than 0.02). These data indicate that basophils in CF may have a greater potential to release mediators although their releasability, expressed as the percentage of histamine release of the total histamine content, does not differ significantly compared to the healthy donors (62.1 +/- 8.3% versus 60.2 +/- 13%, mean +/- s.e.m.). Within the 14-day period of intravenous antibiotic treatment of the pulmonary infection (in 14 out of 15 cases P. aeruginosa was isolated from sputa samples) histamine release per basophil and total histamine content decreased to normal levels (day 1 2.6 +/- 0.3 versus 1.8 +/- 0.3, P less than 0.05). This decline was accompanied by an improvement of the clinical condition of the patient and reduction of P. aeruginosa isolates in sputa (n = 8). In contrast, in three patients with sustained P. aeruginosa colonization of the upper airways and impaired lung function histamine levels remained elevated. Our data demonstrate that the histamine content of basophils, as well as the release of histamine, is increased in patients with cystic fibrosis and correlates with the clinical signs of the chronic infection.

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Selected References

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  1. Cejka J., Mood D. W., Kim C. S. Immunoglobulin concentrations in sera of normal children: quantitation against an international reference preparation. Clin Chem. 1974 Jun;20(6):656–659. [PubMed] [Google Scholar]
  2. Chrispin A. R., Norman A. P. The systematic evaluation of the chest radiograph in cystic fibrosis. Pediatr Radiol. 1974;2(2):101–105. doi: 10.1007/BF01314939. [DOI] [PubMed] [Google Scholar]
  3. Döring G., Høiby N. Longitudinal study of immune response to Pseudomonas aeruginosa antigens in cystic fibrosis. Infect Immun. 1983 Oct;42(1):197–201. doi: 10.1128/iai.42.1.197-201.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GIBSON L. E., COOKE R. E. A test for concentration of electrolytes in sweat in cystic fibrosis of the pancreas utilizing pilocarpine by iontophoresis. Pediatrics. 1959 Mar;23(3):545–549. [PubMed] [Google Scholar]
  5. Gilbert H. S., Ornstein L. Basophil counting with a new staining method using alcian blue. Blood. 1975 Aug;46(2):279–286. [PubMed] [Google Scholar]
  6. Hastie R., Heroy J. H., 3rd, Levy D. A. Basophil leukocytes and mast cells in human nasal secretions and scrapings studied by light microscopy. Lab Invest. 1979 May;40(5):554–561. [PubMed] [Google Scholar]
  7. Ihle J. N., Keller J., Oroszlan S., Henderson L. E., Copeland T. D., Fitch F., Prystowsky M. B., Goldwasser E., Schrader J. W., Palaszynski E. Biologic properties of homogeneous interleukin 3. I. Demonstration of WEHI-3 growth factor activity, mast cell growth factor activity, p cell-stimulating factor activity, colony-stimulating factor activity, and histamine-producing cell-stimulating factor activity. J Immunol. 1983 Jul;131(1):282–287. [PubMed] [Google Scholar]
  8. Ishizaka K. IgE-binding factors and regulation of the IgE antibody response. Annu Rev Immunol. 1988;6:513–534. doi: 10.1146/annurev.iy.06.040188.002501. [DOI] [PubMed] [Google Scholar]
  9. Joseph M., Tonnel A. B., Torpier G., Capron A., Arnoux B., Benveniste J. Involvement of immunoglobulin E in the secretory processes of alveolar macrophages from asthmatic patients. J Clin Invest. 1983 Feb;71(2):221–230. doi: 10.1172/JCI110762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kjellman N. M., Johansson S. G., Roth A. Serum IgE levels in healthy children quantified by a sandwich technique (PRIST). Clin Allergy. 1976 Jan;6(1):51–59. doi: 10.1111/j.1365-2222.1976.tb01411.x. [DOI] [PubMed] [Google Scholar]
  11. Kraemer R., Rüdeberg A., Hadorn B., Rossi E. Relative underweight in cystic fibrosis and its prognostic value. Acta Paediatr Scand. 1978 Jan;67(1):33–37. doi: 10.1111/j.1651-2227.1978.tb16273.x. [DOI] [PubMed] [Google Scholar]
  12. Laharrague P. F., Corberand J. X., Fillola G., Gleizes B. J., Fontanilles A. M., Gyrard E. In vitro effect of the slime of Pseudomonas aeruginosa on the function of human polymorphonuclear neutrophils. Infect Immun. 1984 Jun;44(3):760–762. doi: 10.1128/iai.44.3.760-762.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Liu M. C., Proud D., Lichtenstein L. M., MacGlashan D. W., Jr, Schleimer R. P., Adkinson N. F., Jr, Kagey-Sobotka A., Schulman E. S., Plaut M. Human lung macrophage-derived histamine-releasing activity is due to IgE-dependent factors. J Immunol. 1986 Apr 1;136(7):2588–2595. [PubMed] [Google Scholar]
  14. Owen D. A. Inflammation--histamine and 5-hydroxytryptamine. Br Med Bull. 1987 Apr;43(2):256–269. doi: 10.1093/oxfordjournals.bmb.a072181. [DOI] [PubMed] [Google Scholar]
  15. Paul W. E., Ohara J. B-cell stimulatory factor-1/interleukin 4. Annu Rev Immunol. 1987;5:429–459. doi: 10.1146/annurev.iy.05.040187.002241. [DOI] [PubMed] [Google Scholar]
  16. Rachelefsky G. S., Osher A., Dooley R. E., Ank B., Stiehm E. R. Coexistent respiratory allergy and cystic fibrosis. Am J Dis Child. 1974 Sep;128(3):355–359. doi: 10.1001/archpedi.1974.02110280085010. [DOI] [PubMed] [Google Scholar]
  17. Siraganian R. P. An automated continuous-flow system for the extraction and fluorometric analysis of histamine. Anal Biochem. 1974 Feb;57(2):383–394. doi: 10.1016/0003-2697(74)90093-1. [DOI] [PubMed] [Google Scholar]
  18. Smith C. A., Rennick D. M. Characterization of a murine lymphokine distinct from interleukin 2 and interleukin 3 (IL-3) possessing a T-cell growth factor activity and a mast-cell growth factor activity that synergizes with IL-3. Proc Natl Acad Sci U S A. 1986 Mar;83(6):1857–1861. doi: 10.1073/pnas.83.6.1857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tanner J. M., Whitehouse R. H., Takaishi M. Standards from birth to maturity for height, weight, height velocity, and weight velocity: British children, 1965. II. Arch Dis Child. 1966 Dec;41(220):613–635. doi: 10.1136/adc.41.220.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wood R. E., Boat T. F., Doershuk C. F. Cystic fibrosis. Am Rev Respir Dis. 1976 Jun;113(6):833–878. doi: 10.1164/arrd.1976.113.6.833. [DOI] [PubMed] [Google Scholar]
  21. Zakrzewski J. T., Barnes N. C., Costello J. F., Piper P. J. Lipid mediators in cystic fibrosis and chronic obstructive pulmonary disease. Am Rev Respir Dis. 1987 Sep;136(3):779–782. doi: 10.1164/ajrccm/136.3.779. [DOI] [PubMed] [Google Scholar]

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