Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1989 Jul;77(1):21–24.

Thyroglobulin antibodies in Graves' disease are associated with T-cell receptor beta chain and major histocompatibility complex loci.

A G Demaine 1, S Ratanachaiyavong 1, R Pope 1, D Ewins 1, B A Millward 1, A M McGregor 1
PMCID: PMC1541913  PMID: 2569950

Abstract

We have investigated the T-cell antigen receptor constant beta and alpha chain genes (TCR-C beta, -C alpha) and the immunoglobulin (Ig) heavy chain switch regions of patients with Graves' disease (GD) using restriction fragment length polymorphism (RFLP) analysis. No significant associations were found with RFLPs of either the TCR-C beta, -C alpha or Ig heavy chain switch region loci and GD. However, a significant association was found between the presence of anti-thyroglobulin (anti-Tg) antibodies in the serum of patients and the 10.0; 9.2 kb TCR-C beta genotype (P less than 0.02). Also, those patients with anti-Tg antibodies had an increased frequency of HLA-DR3 (P less than 0.025). These results suggest that genes residing in the TCR chain and major histocompatibility complex loci may be important in determining the immune response to thyroglobulin but not to the disease itself.

Full text

PDF
21

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acha-Orbea H., Mitchell D. J., Timmermann L., Wraith D. C., Tausch G. S., Waldor M. K., Zamvil S. S., McDevitt H. O., Steinman L. Limited heterogeneity of T cell receptors from lymphocytes mediating autoimmune encephalomyelitis allows specific immune intervention. Cell. 1988 Jul 15;54(2):263–273. doi: 10.1016/0092-8674(88)90558-2. [DOI] [PubMed] [Google Scholar]
  2. Beall S. S., Lawrence J. V., Bradley D. A., Mattson D. H., Singer D. S., Biddison W. E. Beta-chain gene rearrangements and V beta gene usage in DPw2-specific T cells. J Immunol. 1987 Aug 15;139(4):1320–1325. [PubMed] [Google Scholar]
  3. Bidwell J. L., Bidwell E. A., Laundy G. J., Klouda P. T., Bradley B. A. Allogenotypes defined by short DQ alpha and DQ beta cDNA probes correlate with and define splits of HLA-DQ serological specificities. Mol Immunol. 1987 May;24(5):513–522. doi: 10.1016/0161-5890(87)90026-5. [DOI] [PubMed] [Google Scholar]
  4. Burman K. D., Baker J. R., Jr Immune mechanisms in Graves' disease. Endocr Rev. 1985 Spring;6(2):183–232. doi: 10.1210/edrv-6-2-183. [DOI] [PubMed] [Google Scholar]
  5. Demaine A. G., Vaughan R. W., Taube D. H., Welsh K. I. Association of membranous nephropathy with T-cell receptor constant beta chain and immunoglobulin heavy chain switch region polymorphisms. Immunogenetics. 1988;27(1):19–23. doi: 10.1007/BF00404439. [DOI] [PubMed] [Google Scholar]
  6. Demaine A., Welsh K. I., Hawe B. S., Farid N. R. Polymorphism of the T cell receptor beta-chain in Graves' disease. J Clin Endocrinol Metab. 1987 Oct;65(4):643–646. doi: 10.1210/jcem-65-4-643. [DOI] [PubMed] [Google Scholar]
  7. Farid N. R., Bear J. C. The human major histocompatibility complex and endocrine disease. Endocr Rev. 1981 Winter;2(1):50–86. doi: 10.1210/edrv-2-1-50. [DOI] [PubMed] [Google Scholar]
  8. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  9. Fink P. J., Matis L. A., McElligott D. L., Bookman M., Hedrick S. M. Correlations between T-cell specificity and the structure of the antigen receptor. Nature. 1986 May 15;321(6067):219–226. doi: 10.1038/321219a0. [DOI] [PubMed] [Google Scholar]
  10. Flanagan J. G., Rabbitts T. H. Arrangement of human immunoglobulin heavy chain constant region genes implies evolutionary duplication of a segment containing gamma, epsilon and alpha genes. Nature. 1982 Dec 23;300(5894):709–713. doi: 10.1038/300709a0. [DOI] [PubMed] [Google Scholar]
  11. Goverman J., Minard K., Shastri N., Hunkapiller T., Hansburg D., Sercarz E., Hood L. Rearranged beta T cell receptor genes in a helper T cell clone specific for lysozyme: no correlation between V beta and MHC restriction. Cell. 1985 Apr;40(4):859–867. doi: 10.1016/0092-8674(85)90345-9. [DOI] [PubMed] [Google Scholar]
  12. Hoover M. L., Capra J. D. HLA and T-cell receptor genes in insulin-dependent diabetes mellitus. Diabetes Metab Rev. 1987 Jul;3(3):835–856. doi: 10.1002/dmr.5610030312. [DOI] [PubMed] [Google Scholar]
  13. Malissen M., McCoy C., Blanc D., Trucy J., Devaux C., Schmitt-Verhulst A. M., Fitch F., Hood L., Malissen B. Direct evidence for chromosomal inversion during T-cell receptor beta-gene rearrangements. Nature. 1986 Jan 2;319(6048):28–33. doi: 10.1038/319028a0. [DOI] [PubMed] [Google Scholar]
  14. Migone N., Feder J., Cann H., van West B., Hwang J., Takahashi N., Honjo T., Piazza A., Cavalli-Sforza L. L. Multiple DNA fragment polymorphisms associated with immunoglobulin mu chain switch-like regions in man. Proc Natl Acad Sci U S A. 1983 Jan;80(2):467–471. doi: 10.1073/pnas.80.2.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Millward B. A., Welsh K. I., Leslie R. D., Pyke D. A., Demaine A. G. T cell receptor beta chain polymorphisms are associated with insulin-dependent diabetes. Clin Exp Immunol. 1987 Oct;70(1):152–157. [PMC free article] [PubMed] [Google Scholar]
  16. Robinson M. A., Kindt T. J. Segregation of polymorphic T-cell receptor genes in human families. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3804–3808. doi: 10.1073/pnas.82.11.3804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. So A., John S., Bailey C., Owen M. J. A new polymorphic marker of the T-cell antigen receptor alpha chain genes in man. Immunogenetics. 1987;25(3):141–144. doi: 10.1007/BF00344026. [DOI] [PubMed] [Google Scholar]
  18. Sorger S. B., Hedrick S. M., Fink P. J., Bookman M. A., Matis L. A. Generation of diversity in T cell receptor repertoire specific for pigeon cytochrome c. J Exp Med. 1987 Feb 1;165(2):279–301. doi: 10.1084/jem.165.2.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  20. Urban J. L., Kumar V., Kono D. H., Gomez C., Horvath S. J., Clayton J., Ando D. G., Sercarz E. E., Hood L. Restricted use of T cell receptor V genes in murine autoimmune encephalomyelitis raises possibilities for antibody therapy. Cell. 1988 Aug 12;54(4):577–592. doi: 10.1016/0092-8674(88)90079-7. [DOI] [PubMed] [Google Scholar]
  21. Weetman A. P., Ratanachaiyavong S., Middleton G. W., Love W., John R., Owen G. M., Darke C., Lazarus J. H., Hall R., McGregor A. M. Prediction of outcome in Graves' disease after carbimazole treatment. Q J Med. 1986 Apr;59(228):409–419. [PubMed] [Google Scholar]
  22. Weetman A. P., So A. K., Roe C., Walport M. J., Foroni L. T-cell receptor alpha chain V region polymorphism linked to primary autoimmune hypothyroidism but not Graves' disease. Hum Immunol. 1987 Oct;20(2):167–173. doi: 10.1016/0198-8859(87)90030-9. [DOI] [PubMed] [Google Scholar]
  23. Weetman A. P., So A. K., Warner C. A., Foroni L., Fells P., Shine B. Immunogenetics of Graves' ophthalmopathy. Clin Endocrinol (Oxf) 1988 Jun;28(6):619–628. doi: 10.1111/j.1365-2265.1988.tb03853.x. [DOI] [PubMed] [Google Scholar]
  24. Yanagi Y., Chan A., Chin B., Minden M., Mak T. W. Analysis of cDNA clones specific for human T cells and the alpha and beta chains of the T-cell receptor heterodimer from a human T-cell line. Proc Natl Acad Sci U S A. 1985 May;82(10):3430–3434. doi: 10.1073/pnas.82.10.3430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yanagi Y., Yoshikai Y., Leggett K., Clark S. P., Aleksander I., Mak T. W. A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains. Nature. 1984 Mar 8;308(5955):145–149. doi: 10.1038/308145a0. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES