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. 1989 Mar;75(3):381–386.

Inhibition of varicella-zoster virus in vitro by human peripheral blood mononuclear cells.

G P Rabalais 1, F E Berkowitz 1, A R Hayward 1, M J Levin 1
PMCID: PMC1541948  PMID: 2539281

Abstract

A functional in vitro assay of cell-mediated immunity to varicella-zoster virus (VZV) is described. This procedure uses an enzyme-linked immunosorbent assay (ELISA) to measure the inhibitory effect of human peripheral blood mononuclear cells on VZV antigen production by VZV-infected cell monolayers. When mononuclear cells from VZV-immune, tetanus-immune donors were stimulated with either VZV antigen or tetanus toxoid they reduced VZV antigen production. In contrast, mononuclear cells from VZV-nonimmune, tetanus-immune donors reduced VZV antigen only when stimulated with tetanus toxoid, but not when stimulated with VZV antigen. Cell-free supernatants recovered from the VZV inhibition assays contained the anti-VZV activity. The magnitude of the anti-VZV activity of the supernatants equalled the inhibition observed when the stimulated mononuclear cells were added to the VZV-infected monolayers. Treatment of either mononuclear cells or supernatants with anti-interferon gamma antibody indicated that their VZV inhibitory capability was largely due to the production of interferon gamma by stimulated mononuclear cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong R. W., Merigan T. C. Varicella zoster virus: interferon production and comparative interferon sensitivity in human cell cultures. J Gen Virol. 1971 Jul;12(1):53–54. doi: 10.1099/0022-1317-12-1-53. [DOI] [PubMed] [Google Scholar]
  2. Arvin A. M., Koropchak C. M., Williams B. R., Grumet F. C., Foung S. K. Early immune response in healthy and immunocompromised subjects with primary varicella-zoster virus infection. J Infect Dis. 1986 Sep;154(3):422–429. doi: 10.1093/infdis/154.3.422. [DOI] [PubMed] [Google Scholar]
  3. Berkowitz F. E., Levin M. J. Use of an enzyme-linked immunosorbent assay performed directly on fixed infected cell monolayers for evaluating drugs against varicella-zoster virus. Antimicrob Agents Chemother. 1985 Aug;28(2):207–210. doi: 10.1128/aac.28.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bowden R. A., McGavren L., Hayward A. R., Levin M. J. Use of bone marrow fibroblasts to prepare targets for an HLA restricted-cytotoxicity assay system. J Clin Microbiol. 1984 Oct;20(4):696–700. doi: 10.1128/jcm.20.4.696-700.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gershon A. A., Steinberg S. P. Cell-mediated immunity to varicella-zoster virus measured by virus inactivation: mechanism and blocking of the reaction by specific antibody. Infect Immun. 1979 Jul;25(1):164–169. doi: 10.1128/iai.25.1.164-169.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gershon A. A., Steinberg S. P. Cellular and humoral immune responses to varicella-zoster virus in immunocompromised patients during and after varicella-zoster infections. Infect Immun. 1979 Jul;25(1):170–174. doi: 10.1128/iai.25.1.170-174.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gershon A. A., Steinberg S. P. Inactivation of varicella zoster virus in vitro: effect of leukocytes and specific antibody. Infect Immun. 1981 Aug;33(2):507–511. doi: 10.1128/iai.33.2.507-511.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gershon A. A., Steinberg S., Smith M. Cell-mediated immunity to varicella-zoster virus demonstrated by viral inactivation with human leukocytes. Infect Immun. 1976 Jun;13(6):1549–1553. doi: 10.1128/iai.13.6.1549-1553.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hayward A. R., Herberger M. Lymphocyte responses to varicella zoster virus in the elderly. J Clin Immunol. 1987 Mar;7(2):174–178. doi: 10.1007/BF00916011. [DOI] [PubMed] [Google Scholar]
  10. Hayward A. R., Pontesilli O., Herberger M., Laszlo M., Levin M. Specific lysis of varicella zoster virus-infected B lymphoblasts by human T cells. J Virol. 1986 Apr;58(1):179–184. doi: 10.1128/jvi.58.1.179-184.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jordan G. W., Merigan T. C. Cell-mediated immunity to varicella-zoster virus: in vitro lymphocyte responses. J Infect Dis. 1974 Nov;130(5):495–501. doi: 10.1093/infdis/130.5.495. [DOI] [PubMed] [Google Scholar]
  12. Kamiya H., Starr S. E., Arbeter A. M., Plotkin S. A. Antibody-dependent cell-mediated cytotoxicity against varicella-zoster virus-infected targets. Infect Immun. 1982 Nov;38(2):554–557. doi: 10.1128/iai.38.2.554-557.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kasahara T., Hooks J. J., Dougherty S. F., Oppenheim J. J. Interleukin 2-mediated immune interferon (IFN-gamma) production by human T cells and T cell subsets. J Immunol. 1983 Apr;130(4):1784–1789. [PubMed] [Google Scholar]
  14. Leibson P. J., Hunter-Laszlo M., Hayward A. R. Inhibition of herpes simplex virus type 1 replication in fibroblast cultures by human blood mononuclear cells. J Virol. 1986 Mar;57(3):976–982. doi: 10.1128/jvi.57.3.976-982.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Merigan T. C., Stevens D. A. Viral infections in man associated with acquired immunological deficiency states. Fed Proc. 1971 Nov-Dec;30(6):1858–1864. [PubMed] [Google Scholar]
  16. Pontesilli O., Carotenuto P., Levin M. J., Suez D., Hayward A. R. Processing and presentation of cell-associated varicella-zoster virus antigens by human monocytes. Clin Exp Immunol. 1987 Oct;70(1):127–135. [PMC free article] [PubMed] [Google Scholar]
  17. Tilden A. B., Cauda R., Grossi C. E., Balch C. M., Lakeman A. D., Whitley R. J. Demonstration of NK cell-mediated lysis of varicella-zoster virus (VZV)-infected cells: characterization of the effector cells. J Immunol. 1986 Jun 1;136(11):4243–4248. [PubMed] [Google Scholar]
  18. Zaia J. A., Leary P. L., Levin M. J. Specificity of the blastogenic response of human mononuclear cells to herpesvirus antigens. Infect Immun. 1978 Jun;20(3):646–651. doi: 10.1128/iai.20.3.646-651.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zaia J. A., Oxman M. N. Antibody to varicella-zoster virus-induced membrane antigen: immunofluorescence assay using monodisperse glutaraldehyde-fixed target cells. J Infect Dis. 1977 Oct;136(4):519–530. doi: 10.1093/infdis/136.4.519. [DOI] [PubMed] [Google Scholar]

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