Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1989 Mar;75(3):349–353.

The detection of anti-Sm-RNP activity in sera of patients with monoclonal gammopathies.

M Abu-Shakrah 1, M Krupp 1, S Argov 1, D Buskila 1, H Slor 1, Y Shoenfeld 1
PMCID: PMC1541950  PMID: 2784742

Abstract

The sera of 141 patients with monoclonal gammopathies were examined for the presence of anti-RNP and anti-Sm activities. An enzyme-linked immunosorbent assay (ELISA) was employed. Thirty-two sera were found to bind RNP while 16 bound to Sm. The anti-Sm and anti-RNP antibodies were found in sera of patients with IgG, IgM and IgA gammopathies. The activity against RNP and/or Sm was confirmed further by using purified immunoglobulins, employing competition assays and immunoblotting. Anti-Sm antibodies are regarded as being highly specific for SLE, yet none of the patients whose serum was found to contain high titres of anti-Sm/anti-RNP antibodies presented with symptoms related to SLE or other rheumatic diseases. Our results of a high incidence of anti-ribonucleoproteins activity in the serum of patients with monoclonal gammopathies support previous reports of autoantibody properties characteristic of these immunoglobulins.

Full text

PDF
349

Images in this article

352Fig. 4
on p.352

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dellagi K., Dupouey P., Brouet J. C., Billecocq A., Gomez D., Clauvel J. P., Seligmann M. Waldenström's macroglobulinemia and peripheral neuropathy: a clinical and immunologic study of 25 patients. Blood. 1983 Aug;62(2):280–285. [PubMed] [Google Scholar]
  2. Dighiero G., Guilbert B., Fermand J. P., Lymberi P., Danon F., Avrameas S. Thirty-six human monoclonal immunoglobulins with antibody activity against cytoskeleton proteins, thyroglobulin, and native DNA: immunologic studies and clinical correlations. Blood. 1983 Aug;62(2):264–270. [PubMed] [Google Scholar]
  3. Dignam J. D., Lebovitz R. M., Roeder R. G. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 1983 Mar 11;11(5):1475–1489. doi: 10.1093/nar/11.5.1475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fermand J. P., Danon F., Brouet J. C. Characterization of a human monoclonal IgM with antibody activity to dsDNA. Clin Exp Immunol. 1985 Feb;59(2):467–474. [PMC free article] [PubMed] [Google Scholar]
  5. Isenberg D. A., Shoenfeld Y., Madaio M. P., Rauch J., Reichlin M., Stollar B. D., Schwartz R. S. Anti-DNA antibody idiotypes in systemic lupus erythematosus. Lancet. 1984 Aug 25;2(8400):417–422. doi: 10.1016/s0140-6736(84)92904-0. [DOI] [PubMed] [Google Scholar]
  6. James J. M., Brouet J. C., Orvoenfrija E., Capron F., Brechot J., Danon F., Diebold J., Rochemaure J., Zittoun R. Waldenström's macroglobulinaemia in a bird breeder: a case history with pulmonary involvement and antibody activity of the monoclonal IgM to canary's droppings. Clin Exp Immunol. 1987 May;68(2):397–401. [PMC free article] [PubMed] [Google Scholar]
  7. Kabat E. A., Liao J., Bretting H., Franklin E. C., Geltner D., Frangione B., Koshland M. E., Shyong J., Osserman E. F. Human monoclonal macroglobulins with specificity for Klebsiella K polysaccharides that contain 3,4-pyruvylated-D-galactose and 4,6-pyruvylated-D-galactose. J Exp Med. 1980 Oct 1;152(4):979–995. doi: 10.1084/jem.152.4.979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Konikoff F., Shoenfeld Y., Isenberg D. A., Barrison I., Sobe T., Theodor E., Slor H. Anti-Rnp antibodies in chronic liver diseases. Clin Exp Rheumatol. 1987 Oct-Dec;5(4):359–361. [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Leibfarth J. H., Persellin R. H. Characteristics of patients with serum antibodies to extractable nuclear antigens. Arthritis Rheum. 1976 Sep-Oct;19(5):851–856. doi: 10.1002/art.1780190503. [DOI] [PubMed] [Google Scholar]
  11. Notman D. D., Kurata N., Tan E. M. Profiles of antinuclear antibodies in systemic rheumatic diseases. Ann Intern Med. 1975 Oct;83(4):464–469. doi: 10.7326/0003-4819-83-4-464. [DOI] [PubMed] [Google Scholar]
  12. Parker M. D. Ribonucleoprotein antibodies: frequency and clinical significance in systemic lupus erythematosus, scleroderma, and mixed connective tissue disease. J Lab Clin Med. 1973 Nov;82(5):769–775. [PubMed] [Google Scholar]
  13. Potter M. Myeloma proteins (M-components) with antibody-like activity. N Engl J Med. 1971 Apr 15;284(15):831–838. doi: 10.1056/NEJM197104152841507. [DOI] [PubMed] [Google Scholar]
  14. Seligmann M., Brouet J. C. Antibody activity of human myeloma globulins. Semin Hematol. 1973 Apr;10(2):163–177. [PubMed] [Google Scholar]
  15. Seligmann M., Danon F., Basch A., Bernard J. IgG myeloma cryoglobulin with antistreptolysin activity. Nature. 1968 Nov 16;220(5168):711–712. doi: 10.1038/220711a0. [DOI] [PubMed] [Google Scholar]
  16. Sestak A. L., Harley J. B., Yoshida S., Reichlin M. Lupus/Sjögren's autoantibody specificities in sera with paraproteins. J Clin Invest. 1987 Jul;80(1):138–144. doi: 10.1172/JCI113039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sharp G. C., Irvin W. S., May C. M., Holman H. R., McDuffie F. C., Hess E. V., Schmid F. R. Association of antibodies to ribonucleoprotein and Sm antigens with mixed connective-tissue disease, systematic lupus erythematosus and other rheumatic diseases. N Engl J Med. 1976 Nov 18;295(21):1149–1154. doi: 10.1056/NEJM197611182952101. [DOI] [PubMed] [Google Scholar]
  18. Shoenfeld Y. Autoimmune diseases: multiple factors involved in the etiology. Isr J Med Sci. 1988 Jul;24(7):351–352. [PubMed] [Google Scholar]
  19. Shoenfeld Y., Ben-Yehuda O., Napartstek Y., Wilner Y., Frolichman R., Schattner A., Lavie G., Joshua H., Pinkhas J., Kennedy R. C. The detection of a common idiotype of anti-DNA antibodies in the sera of patients with monoclonal gammopathies. J Clin Immunol. 1986 May;6(3):194–204. doi: 10.1007/BF00918699. [DOI] [PubMed] [Google Scholar]
  20. Shoenfeld Y., Berliner S., Pinkhas J., Beutler E. The association of Gaucher's disease and dysproteinemias. Acta Haematol. 1980;64(5):241–243. doi: 10.1159/000207271. [DOI] [PubMed] [Google Scholar]
  21. Shoenfeld Y., el-Roeiy A., Ben-Yehuda O., Pick A. I. Detection of anti-histone activity in sera of patients with monoclonal gammopathies. Clin Immunol Immunopathol. 1987 Feb;42(2):250–258. doi: 10.1016/0090-1229(87)90012-2. [DOI] [PubMed] [Google Scholar]
  22. Takano M., Golden S. S., Sharp G. C., Agris P. F. Molecular relationships between two nuclear antigens, ribonucleoprotein and Sm: purification of active antigens and their biochemical characterization. Biochemistry. 1981 Oct 13;20(21):5929–5936. doi: 10.1021/bi00524a001. [DOI] [PubMed] [Google Scholar]
  23. White P. J., Billings P. B., Hoch S. O. Assays for the Sm and RNP autoantigens: the requirement for RNA and influence of the tissue source. J Immunol. 1982 Jun;128(6):2751–2756. [PubMed] [Google Scholar]
  24. Winn D. M., Wolfe J. F., Lindberg D. A., Fristoe F. H., Kingsland L., Sharp G. C. Identification of a clinical subset of systemic lupus erythematosus by antibodies to the SM antigen. Arthritis Rheum. 1979 Dec;22(12):1334–1337. doi: 10.1002/art.1780221203. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES