Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1989 Aug;77(2):179–183.

In vitro IgM and IgM rheumatoid factor production in response to Staphylococcus aureus Cowan I and pokeweed mitogen: the contribution of CD5+ (Leu 1) B cells.

J Karsh 1, R Goldstein 1, A I Lazarovits 1
PMCID: PMC1541978  PMID: 2476269

Abstract

The relation between the percentage of circulating CD5+ CD20+ B cells and the ability to synthesize IgM and IgM rheumatoid factor (RF) in vitro in response to pokeweed mitogen (PWM) and Staphylococcus aureus Cowan I (SAC) was assessed in 21 healthy controls. CD5+ CD20+ cells ranged from 7.3 to 19.9% of total CD20+ B cells. By Spearman's rank correlation, there was an inverse relation between the percentage of CD5+ CD20+ B cells and IgM production in response to PWM (rs = -0.452, p less than 0.05) and a direct correlation with RF production in response to SAC (rs = 0.450, P less than 0.05). The percentage of CD5+ CD20+ B cells was not related to any serologic HLA-A, B, C or D antigen. Healthy individuals may be predisposed to producing IgM or autoantibodies based on the percentage of circulating CD5+ Cd20+ B cells.

Full text

PDF
179

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asano Y., Hodes R. J. T cell regulation of B cell activation. T cells independently regulate the responses mediated by distinct B cell subpopulations. J Exp Med. 1982 May 1;155(5):1267–1276. doi: 10.1084/jem.155.5.1267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Asano Y., Singer A., Hodes R. J. Lyb-5+ B cells can be activated by major histocompatibility complex-restricted as well as unrestricted activation pathways. J Immunol. 1985 Jun;134(6):3682–3685. [PubMed] [Google Scholar]
  3. Carson D. A., Chen P. P., Kipps T. J., Radoux V., Jirik F. R., Goldfien R. D., Fox R. I., Silverman G. J., Fong S. Idiotypic and genetic studies of human rheumatoid factors. Arthritis Rheum. 1987 Dec;30(12):1321–1325. doi: 10.1002/art.1780301201. [DOI] [PubMed] [Google Scholar]
  4. Dixon F. J. ARA Kunkel memorial lecture. Murine lupus. A model for human autoimmunity. Arthritis Rheum. 1985 Oct;28(10):1081–1088. doi: 10.1002/art.1780281002. [DOI] [PubMed] [Google Scholar]
  5. Falkoff R. J., Zhu L. P., Fauci A. S. Separate signals for human B cell proliferation and differentiation in response to Staphylococcus aureus: evidence for a two-signal model of B cell activation. J Immunol. 1982 Jul;129(1):97–102. [PubMed] [Google Scholar]
  6. Ferraccioli G., Karsh J., Osterland C. K. Interaction between fibronectin, rheumatoid factor and aggregated gamma globulins. J Rheumatol. 1985 Aug;12(4):680–684. [PubMed] [Google Scholar]
  7. Fong S., Vaughan J. H., Carson D. A. Two different rheumatoid factor-producing cell populations distinguished by the mouse erythrocyte receptor and responsiveness to polyclonal B cell activators. J Immunol. 1983 Jan;130(1):162–164. [PubMed] [Google Scholar]
  8. Goldstein R., Karsh J. In vitro synthesis of IgM rheumatoid factor in response to Staphylococcus aureus, by lymphocytes from healthy adults. Arthritis Rheum. 1986 Dec;29(12):1440–1445. doi: 10.1002/art.1780291204. [DOI] [PubMed] [Google Scholar]
  9. Goldstein R., Perelmutter L., Karsh J. In vitro IgM and IgM rheumatoid factor production in response to Staphylococcus aureus Cowan I: evidence for the role of Leu-2-positive T suppressor cells and radiosensitive T helper cells. Clin Exp Immunol. 1987 Aug;69(2):358–367. [PMC free article] [PubMed] [Google Scholar]
  10. Hardy R. R., Hayakawa K., Shimizu M., Yamasaki K., Kishimoto T. Rheumatoid factor secretion from human Leu-1+ B cells. Science. 1987 Apr 3;236(4797):81–83. doi: 10.1126/science.3105057. [DOI] [PubMed] [Google Scholar]
  11. Jelinek D. F., Lipsky P. E. Regulation of human B lymphocyte activation, proliferation, and differentiation. Adv Immunol. 1987;40:1–59. doi: 10.1016/s0065-2776(08)60237-0. [DOI] [PubMed] [Google Scholar]
  12. Kipps T. J., Vaughan J. H. Genetic influence on the levels of circulating CD5 B lymphocytes. J Immunol. 1987 Aug 15;139(4):1060–1064. [PubMed] [Google Scholar]
  13. Levinson A. I., Dalal N. F., Haidar M., Tar L., Orlow M. Prominent IgM rheumatoid factor production by human cord blood lymphocytes stimulated in vitro with Staphylococcus aureus Cowan I. J Immunol. 1987 Oct 1;139(7):2237–2241. [PubMed] [Google Scholar]
  14. Levinson A. I., Tar L., Carafa C., Haidar M. Staphylococcus aureus Cowan I. Potent stimulus of immunoglobulin M rheumatoid factor production. J Clin Invest. 1986 Sep;78(3):612–617. doi: 10.1172/JCI112617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MacKenzie M. R., Paglieroni T. G., Warner N. L. Multiple myeloma: an immunologic profile. IV. The EA rosette-forming cell is a Leu-1 positive immunoregulatory B cell. J Immunol. 1987 Jul 1;139(1):24–28. [PubMed] [Google Scholar]
  16. Plater-Zyberk C., Maini R. N., Lam K., Kennedy T. D., Janossy G. A rheumatoid arthritis B cell subset expresses a phenotype similar to that in chronic lymphocytic leukemia. Arthritis Rheum. 1985 Sep;28(9):971–976. doi: 10.1002/art.1780280903. [DOI] [PubMed] [Google Scholar]
  17. Preffer F. I., Colvin R. B., Leary C. P., Boyle L. A., Tuazon T. V., Lazarovits A. I., Cosimi A. B., Kurnick J. T. Two-color flow cytometry and functional analysis of lymphocytes cultured from human renal allografts: identification of a Leu-2+3+ subpopulation. J Immunol. 1986 Nov 1;137(9):2823–2830. [PubMed] [Google Scholar]
  18. Smith H. R., Steinberg A. D. Autoimmunity--a perspective. Annu Rev Immunol. 1983;1:175–210. doi: 10.1146/annurev.iy.01.040183.001135. [DOI] [PubMed] [Google Scholar]
  19. Welch M. J., Fong S., Vaughan J., Carson D. Increased frequency of rheumatoid factor precursor B lymphocytes after immunization of normal adults with tetanus toxoid. Clin Exp Immunol. 1983 Feb;51(2):299–304. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES