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. 1989 Aug;77(2):206–210.

Adherence of alloreactive lymphocytes to human arterial endothelial cell monolayers.

Y L Colson 1, B H Markus 1, A Zeevi 1, R J Duquesnoy 1
PMCID: PMC1541983  PMID: 2789116

Abstract

Early events of cellular infiltration during allograft rejection involve interactions between alloreactive lymphocytes and vascular endothelium. These interactions have been studied in an in vitro model of lymphocyte adherence to human arterial endothelial cell (HAEC) monolayers. Alloreactive lymphocytes primed against donor HLA antigens (DPL) or unrelated HLA antigens from a third party (TPL) were studied for their adherence to HAEC derived from organ transplant donors. DPL exhibited a degree of adherence to donor HAEC which was nearly twice that of the TPL population and developed a morphologically blastoid appearance. DPL adherence was dependent on the recognition of donor HLA antigens expressed on the HAEC surface and investigation of DPL and TPL adherence at various lymphocyte concentrations showed that the binding of DPL and TPL to HAEC was saturable. Monoclonal antibody (MoAb) directed against HLA class I antigens showed inhibition of only DPL adherence to HAEC monolayers expressing donor class I HLA antigens, whereas MoAb directed against other HAEC surface antigens failed to inhibit either the DPL or TPL populations. The results of this study suggest that in a transplant situation, lymphocyte activation and adherence to the graft endothelium is increased in the context of allorecognition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bender J. R., Pardi R., Karasek M. A., Engleman E. G. Phenotypic and functional characterization of lymphocytes that bind human microvascular endothelial cells in vitro. Evidence for preferential binding of natural killer cells. J Clin Invest. 1987 Jun;79(6):1679–1688. doi: 10.1172/JCI113007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Colson Y. L., Markus B. H., Zeevi A., Duquesnoy R. J. Interactions between endothelial cells and alloreactive T cells involved in allograft immunity. Transplant Proc. 1988 Apr;20(2):273–275. [PubMed] [Google Scholar]
  3. Damle N. K., Doyle L. V., Bender J. R., Bradley E. C. Interleukin 2-activated human lymphocytes exhibit enhanced adhesion to normal vascular endothelial cells and cause their lysis. J Immunol. 1987 Mar 15;138(6):1779–1785. [PubMed] [Google Scholar]
  4. Duquesnoy R. J., Zeevi A., Fung J. J., Kaufman C., Zerbe T. R., Griffith B., Trento A., Kormos R., Hardesty R. Sequential infiltration of class I and class II specific alloreactive T cells in human cardiac allografts. Transplant Proc. 1987 Feb;19(1 Pt 3):2560–2563. [PubMed] [Google Scholar]
  5. Fung J. J., Zeevi A., Markus B., Zerbe T. R., Duquesnoy R. J. Dynamics of allospecific T lymphocyte infiltration in vascularized human allografts. Immunol Res. 1986;5(2):149–163. doi: 10.1007/BF02917589. [DOI] [PubMed] [Google Scholar]
  6. Hanto D. W., Hopt U. T., Hoffman R., Simmons R. L. Recruitment of unsensitized circulating lymphocytes to sites of allogeneic cellular interactions. Transplantation. 1982 May;33(5):541–546. doi: 10.1097/00007890-198205000-00015. [DOI] [PubMed] [Google Scholar]
  7. Haskard D., Cavender D., Beatty P., Springer T., Ziff M. T lymphocyte adhesion to endothelial cells: mechanisms demonstrated by anti-LFA-1 monoclonal antibodies. J Immunol. 1986 Nov 1;137(9):2901–2906. [PubMed] [Google Scholar]
  8. Haskard D., Cavender D., Ziff M. Phorbol ester-stimulated T lymphocytes show enhanced adhesion to human endothelial cell monolayers. J Immunol. 1986 Sep 1;137(5):1429–1434. [PubMed] [Google Scholar]
  9. Jarrell B., Levine E., Shapiro S., Williams S., Carabasi R. A., Mueller S., Thornton S. Human adult endothelial cell growth in culture. J Vasc Surg. 1984 Nov;1(6):757–764. doi: 10.1067/mva.1984.avs0010757. [DOI] [PubMed] [Google Scholar]
  10. Markus B. H., Colson Y. L., Fung J. J., Zeevi A., Duquesnoy R. J. HLA antigen expression on cultured human arterial endothelial cells. Tissue Antigens. 1988 Nov;32(5):241–253. doi: 10.1111/j.1399-0039.1988.tb01663.x. [DOI] [PubMed] [Google Scholar]
  11. Miossec P., Cavender D., Ziff M. Production of interleukin 1 by human endothelial cells. J Immunol. 1986 Apr 1;136(7):2486–2491. [PubMed] [Google Scholar]
  12. Pearson J. D., Carleton J. S., Beesley J. E., Hutchings A., Gordon J. L. Granulocyte adhesion to endothelium in culture. J Cell Sci. 1979 Aug;38:225–235. doi: 10.1242/jcs.38.1.225. [DOI] [PubMed] [Google Scholar]
  13. Sheehy M. J., Sondel P. M., Bach M. L., Wank R., Bach F. H. HL-A LD (lymphocyte defined) typing: a rapid assay with primed lymphocytes. Science. 1975 Jun 27;188(4195):1308–1310. doi: 10.1126/science.124948. [DOI] [PubMed] [Google Scholar]
  14. Turner R. R., Beckstead J. H., Warnke R. A., Wood G. S. Endothelial cell phenotypic diversity. In situ demonstration of immunologic and enzymatic heterogeneity that correlates with specific morphologic subtypes. Am J Clin Pathol. 1987 May;87(5):569–575. doi: 10.1093/ajcp/87.5.569. [DOI] [PubMed] [Google Scholar]
  15. WEIBEL E. R., PALADE G. E. NEW CYTOPLASMIC COMPONENTS IN ARTERIAL ENDOTHELIA. J Cell Biol. 1964 Oct;23:101–112. doi: 10.1083/jcb.23.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yu C. L., Haskard D. O., Cavender D., Johnson A. R., Ziff M. Human gamma interferon increases the binding of T lymphocytes to endothelial cells. Clin Exp Immunol. 1985 Dec;62(3):554–560. [PMC free article] [PubMed] [Google Scholar]
  17. Zeevi A., Fung J., Zerbe T. R., Kaufman C., Rabin B. S., Griffith B. P., Hardesty R. L., Duquesnoy R. J. Allospecificity of activated T cells grown from endomyocardial biopsies from heart transplant patients. Transplantation. 1986 May;41(5):620–626. doi: 10.1097/00007890-198605000-00014. [DOI] [PubMed] [Google Scholar]

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