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. 1988 Dec;74(3):321–325.

Induction of the in vitro anti-HBs response by hepatitis B surface antigen.

L G Filion 1, R Saginur 1
PMCID: PMC1542013  PMID: 2976620

Abstract

The production in vitro of antibody to hepatitis B surface antigen by peripheral blood mononuclear cells of healthy volunteers was studied after each of the three doses of hepatitis B vaccine. An in vitro hepatitis B surface antigen antibody response was successfully induced in 12% of the specimens taken over a 7 month period. The response to this antigen was induced in additional samples if cells had been treated previously with anti-CD4 and complement or anti-CD8 and complement prior to culture initiation. The addition of interleukin 2 could also induce the formation of antibodies to hepatitis B surface antigen. The results suggest that the antibody response to hepatitis B surface antigen is complex and varies depending on the individual and time of sampling.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnaba V., Levrero M., Van Dyke A. D., Musca A., Cordova C., Balsano F. T-cell subsets in the hyporesponsiveness to hepatitis B surface antigen (HBsAg) and antigen-specific suppressor lymphocytes in chronic hepatitis B virus (HBV) infection. Clin Immunol Immunopathol. 1985 Mar;34(3):284–295. doi: 10.1016/0090-1229(85)90177-1. [DOI] [PubMed] [Google Scholar]
  2. Barnaba V., Valesini G., Levrero M., Zaccari C., Van Dyke A., Falco M., Musca A., Balsano F. Immunoregulation of the in vitro anti-HBs antibody synthesis in chronic HBsAg carriers and in recently boosted anti-hepatitis B vaccine recipients. Clin Exp Immunol. 1985 May;60(2):259–266. [PMC free article] [PubMed] [Google Scholar]
  3. Celis E., Zurawski V. R., Jr, Chang T. W. Regulation of T-cell function by antibodies: enhancement of the response of human T-cell clones to hepatitis B surface antigen by antigen-specific monoclonal antibodies. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6846–6850. doi: 10.1073/pnas.81.21.6846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chang T. W., Celis E., Miller R. W., Zurawski V. R., Jr, Kung P. C. In vitro response to HBsAg of peripheral blood lymphocytes from recipients of hepatitis B vaccine. Hepatology. 1984 Sep-Oct;4(5):824–829. doi: 10.1002/hep.1840040504. [DOI] [PubMed] [Google Scholar]
  5. Dusheiko G. M., Hoofnagle J. H., Cooksley W. G., James S. P., Jones E. A. Synthesis of antibodies to hepatitis B virus by cultured lymphocytes from chronic hepatitis B surface antigen carriers. J Clin Invest. 1983 May;71(5):1104–1113. doi: 10.1172/JCI110860. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Filion L. G., Saginur R., Szczerbak N. Humoral and cellular immune responses by normal individuals to hepatitis B surface antigen vaccination. Clin Exp Immunol. 1988 Mar;71(3):405–409. [PMC free article] [PubMed] [Google Scholar]
  7. Gerrard T. L., Fauci A. S. Activation and immunoregulation of antigen-specific human b lymphocyte responses: multifaceted role of the monocyte. J Immunol. 1982 May;128(5):2367–2372. [PubMed] [Google Scholar]
  8. Kerlin P., Nies K. M., Tong M. J. Unimpaired B-cell function and T-cell regulation of immunoglobulin synthesis in patients with chronic hepatitis B virus infection. Clin Immunol Immunopathol. 1982 Nov;25(2):149–156. doi: 10.1016/0090-1229(82)90177-5. [DOI] [PubMed] [Google Scholar]
  9. Nowicki M. J., Tong M. J., Bohman R. E. Alterations in the immune response of nonresponders to the hepatitis B vaccine. J Infect Dis. 1985 Dec;152(6):1245–1248. doi: 10.1093/infdis/152.6.1245. [DOI] [PubMed] [Google Scholar]
  10. Sylvan S. P., Hellström U. B., Lundbergh P. R. Detection of cellular and humoral immunity to hepatitis B surface antigen (HBsAg) in asymptomatic HBsAg carriers. Clin Exp Immunol. 1985 Nov;62(2):288–295. [PMC free article] [PubMed] [Google Scholar]
  11. Szmuness W., Stevens C. E., Harley E. J., Zang E. A., Oleszko W. R., William D. C., Sadovsky R., Morrison J. M., Kellner A. Hepatitis B vaccine: demonstration of efficacy in a controlled clinical trial in a high-risk population in the United States. N Engl J Med. 1980 Oct 9;303(15):833–841. doi: 10.1056/NEJM198010093031501. [DOI] [PubMed] [Google Scholar]
  12. Thomas Y., Rogozinski L., Irigoyen O. H., Friedman S. M., Kung P. C., Goldstein G., Chess L. Functional analysis of human T cell subsets defined by monoclonal antibodies. IV. Induction of suppressor cells within the OKT4+ population. J Exp Med. 1981 Aug 1;154(2):459–467. doi: 10.1084/jem.154.2.459. [DOI] [PMC free article] [PubMed] [Google Scholar]

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