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. 1987 Nov;70(2):434–439.

Identification of gp108, a pathogenic antigen of passive Heymann nephritis, as dipeptidyl peptidase IV.

Y Natori 1, I Hayakawa 1, S Shibata 1
PMCID: PMC1542073  PMID: 2892601

Abstract

Our previous paper showed that the glycoprotein of Mr 108,000 (gp108) is one of the major components of rat renal tubular brush border antigens and that an injection of anti-gp108 antiserum in rats induces passive Heymann nephritis with acute and severe proteinuria. In this study, gp108 was identified as a monomer of dipeptidyl peptidase IV (DPP IV). The anti-gp108 antibody was shown to immunoprecipitate DPP IV from Triton-extract of renal tubular membrane fractions. DPP IV was co-purified with gp108 from the Triton-extract by columns of DEAE-cellulose and Bio-gel A-1.5 m. The ratio of DPP IV activity and gp108 content was nearly constant throughout the purification steps. The final purified gp108 showed a high specific activity of DPP IV, comparable to that reported for purified rat DPP IV. These results indicate that gp108 is a monomer of DPP IV.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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