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. 1987 Nov;70(2):417–424.

Terminal complement complex in plasma from patients with systemic lupus erythematosus and other glomerular diseases.

I Horigome 1, J Seino 1, K Sudo 1, Y Kinoshita 1, T Saito 1, K Yoshinaga 1
PMCID: PMC1542074  PMID: 3427827

Abstract

To evaluate terminal complement pathway activation in plasma from patients with systemic lupus erythematosus (SLE) and primary glomerular diseases, we developed an enzyme-linked immunosorbent assay (ELISA) for measuring the terminal complement complexes (TCC). The method is based on a sandwich technique using rabbit antibodies against native human C5, C7 and C9. To avoid interference by native components, we equilibrated plasma specimens with 5% polyethylene glycol buffer. The precipitates were measured by ELISA. TCC was detectable in all 14 normal controls (0.48 +/- 0.06 AU/ml; mean +/- s.e.m.). TCC levels were elevated in 18 of 54 patients with SLE (0.89 +/- 0.07 AU/ml; P less than 0.01) and in eight of 11 patients with membranoproliferative glomerulonephritis (MPGN) (3.15 +/- 0.62 AU/ml; P less than 0.01). However, only one of six patients with membranous nephropathy and none of 13 with mesangial proliferative glomerulonephritis showed high values. In SLE, TCC was correlated with circulating immune complexes and inversely with CH50, C3, C4, C5 and alternative complement pathway activity (AH50), and showed significantly high values even in normal CH50 cases (n = 34; P less than 0.01). In MPGN, TCC was inversely correlated with CH50, AH50, C3, C5 and C9. These results suggest that the classical pathway plays an important role for TCC generation in SLE and that the alternative pathway does in MPGN.

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Selected References

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  1. Bhakdi S., Muhly M. A simple immunoradiometric assay for the terminal SC5b-9 complex of human complement. J Immunol Methods. 1983 Feb 25;57(1-3):283–289. doi: 10.1016/0022-1759(83)90088-1. [DOI] [PubMed] [Google Scholar]
  2. Biesecker G., Katz S., Koffler D. Renal localization of the membrane attack complex in systemic lupus erythematosus nephritis. J Exp Med. 1981 Dec 1;154(6):1779–1794. doi: 10.1084/jem.154.6.1779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cybulsky A. V., Quigg R. J., Salant D. J. The membrane attack complex in complement-mediated glomerular epithelial cell injury: formation and stability of C5b-9 and C5b-7 in rat membranous nephropathy. J Immunol. 1986 Sep 1;137(5):1511–1516. [PubMed] [Google Scholar]
  4. Falk R. J., Dalmasso A. P., Kim Y., Lam S., Michael A. Radioimmunoassay of the attack complex of complement in serum from patients with systemic lupus erythematosus. N Engl J Med. 1985 Jun 20;312(25):1594–1599. doi: 10.1056/NEJM198506203122502. [DOI] [PubMed] [Google Scholar]
  5. Falk R. J., Dalmasso A. P., Kim Y., Tsai C. H., Scheinman J. I., Gewurz H., Michael A. F. Neoantigen of the polymerized ninth component of complement. Characterization of a monoclonal antibody and immunohistochemical localization in renal disease. J Clin Invest. 1983 Aug;72(2):560–573. doi: 10.1172/JCI111004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fukuda K., Seino J., Kinoshita Y., Sudo K., Horigome I., Furuyama T., Yoshinaga K. Modified anti-C3 immune complex assay which avoids interference by anti-F(ab')2 antibodies. Tohoku J Exp Med. 1985 Jul;146(3):337–347. doi: 10.1620/tjem.146.337. [DOI] [PubMed] [Google Scholar]
  7. Gawryl M. S., Simon M. T., Eatman J. L., Lint T. F. An enzyme-linked immunoabsorbent assay for the quantitation of the terminal complement complex from cell membranes or in activated human sera. J Immunol Methods. 1986 Dec 24;95(2):217–225. doi: 10.1016/0022-1759(86)90409-6. [DOI] [PubMed] [Google Scholar]
  8. Hammer C. H., Wirtz G. H., Renfer L., Gresham H. D., Tack B. F. Large scale isolation of functionally active components of the human complement system. J Biol Chem. 1981 Apr 25;256(8):3995–4006. [PubMed] [Google Scholar]
  9. Kolb W. P., Müller-Eberhard H. J. The membrane attack mechanism of complement. Verification of a stable C5-9 complex in free solution. J Exp Med. 1973 Aug 1;138(2):438–451. doi: 10.1084/jem.138.2.438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mollnes T. E., Lea T., Harboe M. Detection and quantification of the terminal C5b-9 complex of human complement by a sensitive enzyme-linked immunosorbent assay. Scand J Immunol. 1984 Aug;20(2):157–166. doi: 10.1111/j.1365-3083.1984.tb00989.x. [DOI] [PubMed] [Google Scholar]
  11. Olds L. C., Miller J. J., 3rd Anti-F(ab')2 antibodies that interfere with interpretation of the anti-C3 assay for immune complexes in children with rheumatic diseases. Arthritis Rheum. 1984 Mar;27(3):330–336. doi: 10.1002/art.1780270313. [DOI] [PubMed] [Google Scholar]
  12. Platts-Mills T. A., Ishizaka K. Activation of the alternate pathway of human complements by rabbit cells. J Immunol. 1974 Jul;113(1):348–358. [PubMed] [Google Scholar]
  13. Podack E. R., Kolb W. P., Muller-Eberhard H. J. The C5b-9 complex: subunit composition of the classical and alternative pathway-generated complex. J Immunol. 1976 May;116(5):1431–1434. [PubMed] [Google Scholar]
  14. Salant D. J., Belok S., Madaio M. P., Couser W. G. A new role for complement in experimental membranous nephropathy in rats. J Clin Invest. 1980 Dec;66(6):1339–1350. doi: 10.1172/JCI109987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sanders M. E., Schmetz M. A., Hammer C. H., Frank M. M., Joiner K. A. Quantitation of activation of the human terminal complement pathway by ELISA. J Immunol Methods. 1985 Dec 27;85(2):245–256. doi: 10.1016/0022-1759(85)90135-8. [DOI] [PubMed] [Google Scholar]
  16. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]
  17. Ware C. F., Wetsel R. A., Kolb W. P. Physicochemical characterization of fluid phase (SC5b-9) and membrane derived (MC5b-9) attack complexes of human complement purified by immunoadsorbent affinity chromatography or selective detergent extraction. Mol Immunol. 1981 Jun;18(6):521–531. doi: 10.1016/0161-5890(81)90130-9. [DOI] [PubMed] [Google Scholar]

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