Abstract
Peripheral blood mononuclear cells (PBMC) from 'responders' recently boosted with hepatitis B vaccine, were studied for synthesis in vitro of antibody to hepatitis B surface antigen (anti-HBs Ab) when stimulated with pokeweed mitogen (PWM) or HBsAg. HBsAg alone can induce an antigen-specific anti-HBs Ab response in vitro; this antibody synthesis is T cell-dependent. In some responders both allogeneic T4+ cells (in absence of PWM or HBsAg) and mixed leucocyte culture supernatants (MLC/SN) (without T cells and antigen) can help responder B cells to produce anti-HBs Ab. Thus, in some immunized subjects, B lymphocytes involved in anti-HBs Ab synthesis are in an advanced phase of differentiation and require only non-antigen specific T cell signals (B cell growth factor or B cell differentiation factor or interleukin 2, etc) to differentiate into antibody-secreting cells. Moreover, the concentration of the antigen necessary to suppress anti-HBs Ab production induced by HBsAg was five times lower than that necessary to suppress antibody production induced by PWM. T cell help for antigen induced anti-HBs Ab could be different from T cell help for the PWM-induced anti-HBs Ab response. Moreover, the finding that the low HBsAg doses inhibiting specific response did not affect the PWM-driven anti-HBs response suggests that antigen-specific T suppressor cells could play a role in this context.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barnaba V., Levrero M., Van Dyke A. D., Musca A., Cordova C., Balsano F. T-cell subsets in the hyporesponsiveness to hepatitis B surface antigen (HBsAg) and antigen-specific suppressor lymphocytes in chronic hepatitis B virus (HBV) infection. Clin Immunol Immunopathol. 1985 Mar;34(3):284–295. doi: 10.1016/0090-1229(85)90177-1. [DOI] [PubMed] [Google Scholar]
- Barnaba V., Valesini G., Levrero M., Zaccari C., Van Dyke A., Falco M., Musca A., Balsano F. Immunoregulation of the in vitro anti-HBs antibody synthesis in chronic HBsAg carriers and in recently boosted anti-hepatitis B vaccine recipients. Clin Exp Immunol. 1985 May;60(2):259–266. [PMC free article] [PubMed] [Google Scholar]
- Cupps T. R., Gerin J. L., Purcell R. H., Goldsmith P. K., Fauci A. S. In vitro antigen-induced antibody responses to hepatitis B surface antigen in man. Kinetic and cellular requirements. J Clin Invest. 1984 Oct;74(4):1204–1213. doi: 10.1172/JCI111529. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kehrl J. H., Muraguchi A., Butler J. L., Falkoff R. J., Fauci A. S. Human B cell activation, proliferation and differentiation. Immunol Rev. 1984 Apr;78:75–96. doi: 10.1111/j.1600-065x.1984.tb00477.x. [DOI] [PubMed] [Google Scholar]
- Lum L. G., Culbertson N. J. The induction and suppression of in vitro IgG anti-tetanus toxoid antibody synthesis by human lymphocytes stimulated with tetanus toxoid in the absence of in vivo booster immunizations. J Immunol. 1985 Jul;135(1):185–191. [PubMed] [Google Scholar]
- Mage M. G., McHugh L. L., Rothstein T. L. Mouse lymphocytes with and without surface immunoglobulin: preparative scale separation in polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin. J Immunol Methods. 1977;15(1):47–56. doi: 10.1016/0022-1759(77)90016-3. [DOI] [PubMed] [Google Scholar]
- Peters M., Fauci A. S. Selective activation of antigen-specific human B cells in recently immunized individuals by nonspecific factors in the absence of antigen. J Immunol. 1983 Feb;130(2):678–680. [PubMed] [Google Scholar]
- Stevens R. H. Immunoglobulin-bearing cells are a target for the antigen-induced inhibition of pokeweed mitogen-stimulated antibody production. J Immunol. 1981 Sep;127(3):968–972. [PubMed] [Google Scholar]
- Volkman D. J., Allyn S. P., Fauci A. S. Antigen-induced in vitro antibody production in humans: tetanus toxoid-specific antibody synthesis. J Immunol. 1982 Jul;129(1):107–112. [PubMed] [Google Scholar]
- Wysocki L. J., Sato V. L. "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844–2848. doi: 10.1073/pnas.75.6.2844. [DOI] [PMC free article] [PubMed] [Google Scholar]