Skip to main content
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1986 Apr;64(1):14–19.

Characterization of the humoral immune response in Sudanese leishmaniasis: specific antibody detected by class- and subclass-specific reagents.

E M el Amin, E P Wright, A Vlug
PMCID: PMC1542154  PMID: 3731524

Abstract

Sera from Sudanese patients with either visceral or mucosal manifestations of infection with Leishmania were investigated to determine the class of antibodies against the causative parasite in an enzyme-linked immunosorbent assay (ELISA) with intact promastigotes as antigen. All patient sera had significantly more IgG and more antiparasite IgG than did control Sudanese or control Dutch sera; little or no IgM or IgA antiparasite antibody activity was detected. When sera specific for the subclasses of IgG were used to detect the anti-promastigote antibodies, these were found in IgG1- and IgG3-specific ELISA but not in those for IgG2 or IgG4.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aalberse R. C., van der Gaag R., van Leeuwen J. Serologic aspects of IgG4 antibodies. I. Prolonged immunization results in an IgG4-restricted response. J Immunol. 1983 Feb;130(2):722–726. [PubMed] [Google Scholar]
  2. Beck O. E. Distribution of virus antibody activity among human IgG subclasses. Clin Exp Immunol. 1981 Mar;43(3):626–632. [PMC free article] [PubMed] [Google Scholar]
  3. Bird P., Lowe J., Stokes R. P., Bird A. G., Ling N. R., Jefferis R. The separation of human serum IgG into subclass fractions by immunoaffinity chromatography and assessment of specific antibody activity. J Immunol Methods. 1984 Jun 8;71(1):97–105. doi: 10.1016/0022-1759(84)90209-6. [DOI] [PubMed] [Google Scholar]
  4. Chang K. P. Antibody-mediated inhibition of phagocytosis in Leishmania donovani-human phagocyte interactions in vitro. Am J Trop Med Hyg. 1981 Mar;30(2):334–339. doi: 10.4269/ajtmh.1981.30.334. [DOI] [PubMed] [Google Scholar]
  5. French M. A., Harrison G. Serum IgG subclass concentrations in healthy adults: a study using monoclonal antisera. Clin Exp Immunol. 1984 May;56(2):473–475. [PMC free article] [PubMed] [Google Scholar]
  6. Ghose A. C., Haldar J. P., Pal S. C., Mishra B. P., Mishra K. K. Serological investigations on Indian kala-azar. Clin Exp Immunol. 1980 May;40(2):318–326. [PMC free article] [PubMed] [Google Scholar]
  7. Goosen P. C., Van Beekhuizen S., Droogh C., De Lange G. Preparation of antibodies against subclasses of human IgG. J Immunol Methods. 1981;40(3):339–344. doi: 10.1016/0022-1759(81)90365-3. [DOI] [PubMed] [Google Scholar]
  8. Heiner D. C. Significance of immunoglobulin G subclasses. Am J Med. 1984 Mar 30;76(3A):1–6. doi: 10.1016/0002-9343(84)90313-9. [DOI] [PubMed] [Google Scholar]
  9. Ho M., Leeuwenburg J., Mbugua G., Wamachi A., Voller A. An enzyme-linked immunosorbent assay (ELISA) for field diagnosis of visceral leishmaniasis. Am J Trop Med Hyg. 1983 Sep;32(5):943–946. doi: 10.4269/ajtmh.1983.32.943. [DOI] [PubMed] [Google Scholar]
  10. Hommel M., Peters W., Ranque J., Quilici M., Lanotte G. The micro-ELISA technique in the serodiagnosis of visceral leishmaniasis. Ann Trop Med Parasitol. 1978 Jun;72(3):213–218. doi: 10.1080/00034983.1978.11719308. [DOI] [PubMed] [Google Scholar]
  11. Iskander R., Das P. K., Aalberse R. C. IgG4 antibodies in Egyptian patients with schistosomiasis. Int Arch Allergy Appl Immunol. 1981;66(2):200–207. doi: 10.1159/000232819. [DOI] [PubMed] [Google Scholar]
  12. Kaschka W. P., Klein G., Hilgers R., Skvaril F. Humoral immune response in Epstein-Barr virus infections. II. IgG subclass distribution in African patients with Burkitt's lymphoma and nasopharyngeal carcinoma. Clin Exp Immunol. 1984 Jan;55(1):14–22. [PMC free article] [PubMed] [Google Scholar]
  13. Klein F., Skvaril F., Vermeeren R., Vlug A., Duimel W. J. The quantification of human IgG subclasses in reference preparations. Clin Chim Acta. 1985 Aug 15;150(2):119–127. doi: 10.1016/0009-8981(85)90262-1. [DOI] [PubMed] [Google Scholar]
  14. Mohammed E. A., Wright E. P., Kager P. A., Laarman J. J., Pondman K. W. ELISA using intact promastigotes for immunodiagnosis of kala-azar. Trans R Soc Trop Med Hyg. 1985;79(3):344–350. doi: 10.1016/0035-9203(85)90376-1. [DOI] [PubMed] [Google Scholar]
  15. Mortimer G. E., Widdowson J. P. Predominance of immunoglobulin G sub-class 3 among the complement-fixing antibodies to streptococcal M-associated protein. Clin Exp Immunol. 1979 Aug;37(2):247–258. [PMC free article] [PubMed] [Google Scholar]
  16. Pearson R. D., Steigbigel R. T. Mechanism of lethal effect of human serum upon Leishmania donovani. J Immunol. 1980 Nov;125(5):2195–2201. [PubMed] [Google Scholar]
  17. Pearson R. D., Wheeler D. A., Harrison L. H., Kay H. D. The immunobiology of leishmaniasis. Rev Infect Dis. 1983 Sep-Oct;5(5):907–927. doi: 10.1093/clinids/5.5.907. [DOI] [PubMed] [Google Scholar]
  18. Rezai H. R., Ardehali S., Gettner S. Anti-leishmania activity of normal animal sera. Ann Trop Med Parasitol. 1975 Mar;69(1):29–33. doi: 10.1080/00034983.1975.11686981. [DOI] [PubMed] [Google Scholar]
  19. Scott M. T., Goss-Sampson M. Restricted IgG isotype profiles in T. cruzi infected mice and Chagas' disease patients. Clin Exp Immunol. 1984 Nov;58(2):372–379. [PMC free article] [PubMed] [Google Scholar]
  20. Shakib F., McLaughlan P., Stanworth D. R., Smith E., Fairburn E. Elevated serum IgE and IgG4 in patients with atopic dermatitis. Br J Dermatol. 1977 Jul;97(1):59–63. doi: 10.1111/j.1365-2133.1977.tb15428.x. [DOI] [PubMed] [Google Scholar]
  21. Siber G. R., Schur P. H., Aisenberg A. C., Weitzman S. A., Schiffman G. Correlation between serum IgG-2 concentrations and the antibody response to bacterial polysaccharide antigens. N Engl J Med. 1980 Jul 24;303(4):178–182. doi: 10.1056/NEJM198007243030402. [DOI] [PubMed] [Google Scholar]
  22. Wahlgren M., Berzins K., Perlmann P., Persson M. Characterization of the humoral immune response in Plasmodium falciparum malaria. II. IgG subclass levels of anti-P. falciparum antibodies in different sera. Clin Exp Immunol. 1983 Oct;54(1):135–142. [PMC free article] [PubMed] [Google Scholar]
  23. van der Giessen M., Groenboer-Kempers O. The subclasses of human IgG antibodies against tetanus toxoid. Clin Exp Immunol. 1976 Jul;25(1):117–121. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES