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. 1986 Apr;64(1):214–222.

Novel neutrophil chemotactic factor derived from human peripheral blood mononuclear leucocytes.

E Kownatzki, A Kapp, S Uhrich
PMCID: PMC1542167  PMID: 3731527

Abstract

Human mononuclear leucocytes isolated from the peripheral blood by centrifugation on Ficoll-Hypaque cushions and adherent on plastic petri dishes, produced a chemotactic factor that attracted human neutrophilic granulocytes to the same extent as did optimal concentrations of the complement split product C5a and the leukotriene B4. The active component eluted from a Sephadex G-50 gel filtration column as a single peak with an apparent molecular weight of 10,000. The chemotactic activity was resistant to reductive cleavage of disulfide bonds and heating at 100 degrees C for 30 min but was lost when reduction and heating were combined. Digestion with a proteolytic enzyme eliminated the attractive potential. The data suggest that this is a novel chemotactic peptide. It is conceivable that it has been seen previously and was mistaken for a lymphokine or interleukin 1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altman L. C., Snyderman R., Oppenheim J. J., Mergenhagen S. E. A human mononuclear leukocyte chemotactic factor: characterization, specificity and kinetics of production by homologous leukocytes. J Immunol. 1973 Mar;110(3):801–810. [PubMed] [Google Scholar]
  2. Andrews P. Estimation of molecular size and molecular weights of biological compounds by gel filtration. Methods Biochem Anal. 1970;18:1–53. [PubMed] [Google Scholar]
  3. Andrews P. The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochem J. 1965 Sep;96(3):595–606. doi: 10.1042/bj0960595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BOYDEN S. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med. 1962 Mar 1;115:453–466. doi: 10.1084/jem.115.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bokisch V. A., Müller-Eberhard H. J. Anaphylatoxin inactivator of human plasma: its isolation and characterization as a carboxypeptidase. J Clin Invest. 1970 Dec;49(12):2427–2436. doi: 10.1172/JCI106462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Borgeat P., Samuelsson B. Arachidonic acid metabolism in polymorphonuclear leukocytes: effects of ionophore A23187. Proc Natl Acad Sci U S A. 1979 May;76(5):2148–2152. doi: 10.1073/pnas.76.5.2148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chodirker W. B., Bock G. N., Vaughan J. H. Isolation of human PMN leukocytes and granules: observations on early blood diluion and on heparin. J Lab Clin Med. 1968 Jan;71(1):9–19. [PubMed] [Google Scholar]
  8. Czarnetzki B. M. Low molecular weight eosinophil chemotactic factor (ECF) production by rat peritoneal mononuclear phagocytes. Immunobiology. 1980 Apr;157(1):62–66. doi: 10.1016/S0171-2985(80)80063-5. [DOI] [PubMed] [Google Scholar]
  9. Damerau B., Wüstefeld H., Vogt W. Binding characteristics of the complement peptides C3a and C5a-desArg to cellulose nitrate filters in Boyden chambers. Agents Actions Suppl. 1983;12:121–133. doi: 10.1007/978-3-0348-9352-7_7. [DOI] [PubMed] [Google Scholar]
  10. Ford-Hutchinson A. W., Bray M. A., Doig M. V., Shipley M. E., Smith M. J. Leukotriene B, a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytes. Nature. 1980 Jul 17;286(5770):264–265. doi: 10.1038/286264a0. [DOI] [PubMed] [Google Scholar]
  11. Galanos C., Lüderitz O. Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms. Eur J Biochem. 1975 Jun;54(2):603–610. doi: 10.1111/j.1432-1033.1975.tb04172.x. [DOI] [PubMed] [Google Scholar]
  12. Gerard C., Hugli T. E. Identification of classical anaphylatoxin as the des-Arg form of the C5a molecule: evidence of a modulator role for the oligosaccharide unit in human des-Arg74-C5a. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1833–1837. doi: 10.1073/pnas.78.3.1833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Grabbe J., Czarnetzki B. M., Rosenbach T., Mardin M. Identification of chemotactic lipoxygenase products of arachidonate metabolism in psoriatic skin. J Invest Dermatol. 1984 May;82(5):477–479. doi: 10.1111/1523-1747.ep12260985. [DOI] [PubMed] [Google Scholar]
  14. HANKS J. H., WALLACE J. H. Determination of cell viability. Proc Soc Exp Biol Med. 1958 May;98(1):188–192. doi: 10.3181/00379727-98-23985. [DOI] [PubMed] [Google Scholar]
  15. Hugli T. E. The structural basis for anaphylatoxin and chemotactic functions of C3a, C4a, and C5a. Crit Rev Immunol. 1981 Feb;1(4):321–366. [PubMed] [Google Scholar]
  16. Hunninghake G. W., Gadek J. E., Fales H. M., Crystal R. G. Human alveolar macrophage-derived chemotactic factor for neutrophils. Stimuli and partial characterization. J Clin Invest. 1980 Sep;66(3):473–483. doi: 10.1172/JCI109878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hunninghake G. W., Gadek J. E., Lawley T. J., Crystal R. G. Mechanisms of neutrophil accumulation in the lungs of patients with idiopathic pulmonary fibrosis. J Clin Invest. 1981 Jul;68(1):259–269. doi: 10.1172/JCI110242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kazmierowski J. A., Gallin J. I., Reynolds H. Y. Mechanism for the inflammatory response in primate lungs. Demonstration and partial characterization of an alveolar macrophage-derived chemotactic factor with preferential activity for polymorphonuclear leukocytes. J Clin Invest. 1977 Feb;59(2):273–281. doi: 10.1172/JCI108638. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Keller H. U., Wilkinson P. C., Abercrombie M., Becker E. L., Hirsch J. G., Miller M. E., Scottramsey W., Zigmond S. H. A proposal for the definition of terms related to locomotion of leucocytes and other cells. Clin Exp Immunol. 1977 Mar;27(3):377–380. [PMC free article] [PubMed] [Google Scholar]
  20. Luger T. A., Stadler B. M., Luger B. M., Sztein M. B., Schmidt J. A., Hawley-Nelson P., Grabner G., Oppenheim J. J. Characteristics of an epidermal cell thymocyte-activating factor (ETAF) produced by human epidermal cells and a human squamous cell carcinoma cell line. J Invest Dermatol. 1983 Sep;81(3):187–193. doi: 10.1111/1523-1747.ep12517658. [DOI] [PubMed] [Google Scholar]
  21. Meltzer M. S., Oppenheim J. J. Bidirectional amplification of macrophage-lymphocyte interactions: enhanced lymphocyte activation factor production by activated adherent mouse peritoneal cells. J Immunol. 1977 Jan;118(1):77–82. [PubMed] [Google Scholar]
  22. Merrill W. W., Naegel G. P., Matthay R. A., Reynolds H. Y. Alveolar macrophage-derived chemotactic factor: kinetics of in vitro production and partial characterization. J Clin Invest. 1980 Feb;65(2):268–276. doi: 10.1172/JCI109668. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mizel S. B., Mizel D. Purification to apparent homogeneity of murine interleukin 1. J Immunol. 1981 Mar;126(3):834–837. [PubMed] [Google Scholar]
  24. Rocklin R. E., Bendtzen K., Greineder D. Mediators of immunity: lymphokines and monokines. Adv Immunol. 1980;29:55–136. doi: 10.1016/s0065-2776(08)60043-7. [DOI] [PubMed] [Google Scholar]
  25. Rosenwasser L. J., Dinarello C. A., Rosenthal A. S. Adherent cell function in murine T-lymphocyte antigen recognition. IV. Enhancement of murine T-cell antigen recognition by human leukocytic pyrogen. J Exp Med. 1979 Sep 19;150(3):709–714. doi: 10.1084/jem.150.3.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sauder D. N., Mounessa N. L., Katz S. I., Dinarello C. A., Gallin J. I. Chemotactic cytokines: the role of leukocytic pyrogen and epidermal cell thymocyte-activating factor in neutrophil chemotaxis. J Immunol. 1984 Feb;132(2):828–832. [PubMed] [Google Scholar]
  27. Schiffmann E., Corcoran B. A., Wahl S. M. N-formylmethionyl peptides as chemoattractants for leucocytes. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1059–1062. doi: 10.1073/pnas.72.3.1059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schiffmann E., Gallin J. I. Biochemistry of phagocyte chemotaxis. Curr Top Cell Regul. 1979;15:203–261. doi: 10.1016/b978-0-12-152815-7.50010-7. [DOI] [PubMed] [Google Scholar]
  29. Snyderman R., Goetzl E. J. Molecular and cellular mechanisms of leukocyte chemotaxis. Science. 1981 Aug 21;213(4510):830–837. doi: 10.1126/science.6266014. [DOI] [PubMed] [Google Scholar]
  30. Snyderman R., Meadows L., Amos D. B. Characterization of human chemotactic lymphokine production induced by mitogens and mixed leukocyte reactions using a new microassay. Cell Immunol. 1977 May;30(2):225–235. doi: 10.1016/0008-8749(77)90067-3. [DOI] [PubMed] [Google Scholar]
  31. Tucker S. B., Pierre R. V., Jordon R. E. Rapid identification of monocytes in a mixed mononuclear cell preparation. J Immunol Methods. 1977;14(3-4):267–269. doi: 10.1016/0022-1759(77)90137-5. [DOI] [PubMed] [Google Scholar]
  32. Valone F. H., Franklin M., Sun F. F., Goetzl E. J. Alveolar macrophage lipoxygenase products of arachidonic acid: isolation and recognition as the predominant constituents of the neutrophil chemotactic activity elaborated by alveolar macrophages. Cell Immunol. 1980 Sep 1;54(2):390–401. doi: 10.1016/0008-8749(80)90219-1. [DOI] [PubMed] [Google Scholar]
  33. Zigmond S. H., Hirsch J. G. Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. J Exp Med. 1973 Feb 1;137(2):387–410. doi: 10.1084/jem.137.2.387. [DOI] [PMC free article] [PubMed] [Google Scholar]

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