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. 1987 Dec;70(3):529–537.

Comparison between autoantibodies in malaria and leprosy with lupus.

E Bonfa 1, R Llovet 1, M Scheinberg 1, J M de Souza 1, K B Elkon 1
PMCID: PMC1542174  PMID: 3325202

Abstract

Sera from 16 patients with falciparum malaria, 16 patients with vivax malaria and 31 patients with leprosy were tested for autoantibodies to intracellular proteins and nucleic acids. Precipitating antibodies to soluble protein extracts were not detected in any serum. Sera from malaria patients showed prominent immunofluorescence staining of the HEP2 nuclear membrane as well as frequent 75% (24/32) and intense Western blot reactivity. In contrast, only 20% and 36% of patients with leprosy had positive immunofluorescence or positive immunoblots respectively, and reactivity was weak in most cases. Neither the malaria nor leprosy sera contained autoantibodies with specificities similar to the characteristic lupus autoantibodies such as double stranded DNA (dsDNA), Ro/SSA, La/SSB, Sm, RNP and P proteins. Low levels of antibodies to single stranded (ssDNA) were however found in 11 (34%) malaria sera and in seven (23%) leprosy sera. Thirteen percent of patients with leprosy had anti-histone antibodies. These findings demonstrate considerable differences in the capacity of infectious agents to induce autoantibodies and also the infrequency with which autoantibodies characteristic of idiopathic systemic lupus erythematosus are induced.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adu D., Williams D. G., Quakyi I. A., Voller A., Anim-Addo Y., Bruce-Tagoe A. A., Johnson G. D., Holborow E. J. Anti-ssDNA and antinuclear antibodies in human malaria. Clin Exp Immunol. 1982 Aug;49(2):310–316. [PMC free article] [PubMed] [Google Scholar]
  2. Anders R. F., Shi P. T., Scanlon D. B., Leach S. J., Coppel R. L., Brown G. V., Stahl H. D., Kemp D. J. Antigenic repeat structures in proteins of Plasmodium falciparum. Ciba Found Symp. 1986;119:164–183. doi: 10.1002/9780470513286.ch10. [DOI] [PubMed] [Google Scholar]
  3. BONOMO L., TURSI A., TRIMIGLIOZZI G., DAMMACCO F. L.E. CELLS AND ANTINUCLEAR FACTORS IN LEPROSY. Br Med J. 1965 Sep 18;2(5463):689–690. doi: 10.1136/bmj.2.5463.689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bahr G. M., Rook G. A., Moreno E., Lydyard P. M., Modabber F. Z., Stanford J. L. Use of the ELISA to screen for anti-thymocyte and anti-beta 2-microglobulin antibodies in leprosy and SLE. Immunology. 1980 Dec;41(4):865–873. [PMC free article] [PubMed] [Google Scholar]
  5. Boonpucknavig S., Ekapanyakul G. Autoantibodies in sera of Thai patients with Plasmodium falciparum infection. Clin Exp Immunol. 1984 Oct;58(1):77–82. [PMC free article] [PubMed] [Google Scholar]
  6. Christian C. L., Elkon K. B. Autoantibodies to intracellular proteins. Clinical and biologic significance. Am J Med. 1986 Jan;80(1):53–61. doi: 10.1016/0002-9343(86)90048-3. [DOI] [PubMed] [Google Scholar]
  7. Elkon K. B., Culhane L. Partial immunochemical characterization of the Ro and La proteins using antibodies from patients with the sicca syndrome and lupus erythematosus. J Immunol. 1984 May;132(5):2350–2356. [PubMed] [Google Scholar]
  8. Elkon K. B., Inman R. D., Culhane L., Christian C. L. Induction of polymeric IgA rheumatoid factors in infective endocarditis. Am J Med. 1983 Nov;75(5):785–789. doi: 10.1016/0002-9343(83)90408-4. [DOI] [PubMed] [Google Scholar]
  9. Elkon K. B., Jankowski P. W. Fine specificities of autoantibodies directed against the Ro, La, Sm, RNP, and Jo-1 proteins defined by two-dimensional gel electrophoresis and immunoblotting. J Immunol. 1985 Jun;134(6):3819–3824. [PubMed] [Google Scholar]
  10. Greenwood B. M. Possible role of a B-cell mitogen in hypergammaglobulinaemia in malaria and trypanosomiasis. Lancet. 1974 Mar 16;1(7855):435–436. doi: 10.1016/s0140-6736(74)92386-1. [DOI] [PubMed] [Google Scholar]
  11. Hommel M., David P. H., Oligino L. D. Surface alterations of erythrocytes in Plasmodium falciparum malaria. Antigenic variation, antigenic diversity, and the role of the spleen. J Exp Med. 1983 Apr 1;157(4):1137–1148. doi: 10.1084/jem.157.4.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Koffler D., Carr R. I., Agnello V., Fiezi T., Kunkel H. G. Antibodies to polynucleotides: distribution in human serums. Science. 1969 Dec 26;166(3913):1648–1649. doi: 10.1126/science.166.3913.1648. [DOI] [PubMed] [Google Scholar]
  13. Kreisler J. M., Arnaiz A., Perez B., Bootello A. Lumphocytotoxins in leprosy. Int J Lepr Other Mycobact Dis. 1975 Apr-Jun;43(2):91–94. [PubMed] [Google Scholar]
  14. Kurata N., Tan E. M. Identification of antibodies to nuclear acidic antigens by counterimmunoelectrophoresis. Arthritis Rheum. 1976 May-Jun;19(3):574–580. doi: 10.1002/art.1780190309. [DOI] [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Mackworth-Young C. G., Chan J. K., Bunn C. C., Hughes G. R., Gharavi A. E. Complement fixation by anti-dsDNA antibodies in SLE: measurement by radioimmunoassay and relationship with disease activity. Ann Rheum Dis. 1986 Apr;45(4):314–318. doi: 10.1136/ard.45.4.314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Petchclai B., Chuthanondh R., Rungruong S., Ramasoota T. Autoantibodies in leprosy among Thai patients. Lancet. 1973 Jun 30;1(7818):1481–1482. doi: 10.1016/s0140-6736(73)91816-3. [DOI] [PubMed] [Google Scholar]
  18. Phanuphak P., Tirawatnpong S., Hanvanich M., Panmuong W., Moollaor P., Vejjajiva S., Sitprija V., Intaraprasert R., Phanthumkosol D. Autoantibodies in falciparum malaria: a sequential study in 183 Thai patients. Clin Exp Immunol. 1983 Sep;53(3):627–633. [PMC free article] [PubMed] [Google Scholar]
  19. Rea T. H., Quismorio F. P., Harding B., Nies K. M., Di Saia P. J., Levan N. E., Friou G. J. Immunologic responses in patients with lepromatous leprosy. Arch Dermatol. 1976 Jun;112(6):791–800. [PubMed] [Google Scholar]
  20. Rosenberg Y. J. Autoimmune and polyclonal B cell responses during murine malaria. Nature. 1978 Jul 13;274(5667):170–172. doi: 10.1038/274170a0. [DOI] [PubMed] [Google Scholar]
  21. Saha K., Dutta R. N., Mittal M. M. Immunologic aspects of leprosy as related to leucocytic isoantibodies and platelet aggregating factors. Int J Lepr Other Mycobact Dis. 1975 Jul-Sep;43(3):239–248. [PubMed] [Google Scholar]
  22. Shwe T. Clinical significance of autoimmune antibodies in leprosy. Trans R Soc Trop Med Hyg. 1972;66(5):749–753. doi: 10.1016/0035-9203(72)90089-2. [DOI] [PubMed] [Google Scholar]
  23. Tan E. M. Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine. Adv Immunol. 1982;33:167–240. doi: 10.1016/s0065-2776(08)60836-6. [DOI] [PubMed] [Google Scholar]
  24. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wager O. Immunological aspects of leprosy with special reference to autoimmune diseases. Bull World Health Organ. 1969;41(6):793–804. [PMC free article] [PubMed] [Google Scholar]

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