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. 1987 Oct;70(1):18–25.

Use of monoclonal antibodies to identify shared idiotypes on anticardiolipin and anti-DNA antibodies in human sera.

G Valesini 1, A Tincani 1, E N Harris 1, P G Mantelli 1, F Allegri 1, G Palmieri 1, G R Hughes 1, F Balsano 1, G Balestrieri 1
PMCID: PMC1542216  PMID: 3500812

Abstract

Using hybridoma technology we produced monoclonal antibodies (MoAb) to idiotypic determinants on human anti-cardiolipin antibodies purified from a patient with SLE. Hybridomas were screened by inhibition of cardiolipin binding activity of sera from patients with SLE. Seven hybridomas were selected, two of which were studied extensively. Sera from a number of patients with SLE were found to share idiotypic determinants. This cross-reacting idiotype was not detectable on anti-cardiolipin antibodies in syphilis sera. The cross-reacting idiotype was present in sera with anti-ssDNA antibodies even though some of these sera had no anti-cardiolipin antibodies. We propose that these MoAb may recognize a regulatory idiotype.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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