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. 1987 Jul;69(1):25–32.

Characterization of different types of experimental autoimmune thyroiditis in the Buffalo strain rat.

S B Cohen 1, A P Weetman 1
PMCID: PMC1542250  PMID: 3652530

Abstract

We have compared features of experimental thyroiditis in the Buffalo strain rat induced by neonatal thymectomy, immunization with rat thyroglobulin (Tg) and complete Freund's adjuvant, and subcutaneous administration of trypan blue or 3-methylcholanthrene. The disease produced by neonatal thymectomy resulted in significantly worse thyroiditis and higher antibody levels than the other models and shared other features in common with Hashimoto's thyroiditis. In particular TSH levels were elevated, Tg antibodies had a restricted subclass distribution, the thyroid was infiltrated by both B cells and T cells (comprising equal numbers of W3/25 and Ox8 positive cells) and thyroid follicular cells were Ia antigen-positive in some of the thymectomy group animals. In the other forms of thyroiditis, the thyroid infiltrate was mainly composed of macrophages or dendritic cells and B cells. Different pathogenic mechanisms are probably involved in these models; the disease produced by neonatal thymectomy shows the closest similarity to Hashimoto's thyroiditis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aichinger G., Fill H., Wick G. In situ immune complexes, lymphocyte subpopulations, and HLA-DR-positive epithelial cells in Hashimoto thyroiditis. Lab Invest. 1985 Feb;52(2):132–140. [PubMed] [Google Scholar]
  2. Bazin H., Beckers A., Querinjean P. Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur J Immunol. 1974 Jan;4(1):44–48. doi: 10.1002/eji.1830040112. [DOI] [PubMed] [Google Scholar]
  3. FREEMAN T., GORDON A. H., HUMPHREY J. H. Distinction between catabolism of native and denatured proteins by the isolated perfused liver after carbon loading. Br J Exp Pathol. 1958 Oct;39(5):459–471. [PMC free article] [PubMed] [Google Scholar]
  4. Glover E. L., Reuber M. D., Grollman S. Influence of age and sex on thyroiditis in rats injected subcutaneously with 3-methylcholanthrene. Pathol Microbiol (Basel) 1968;32(6):314–320. doi: 10.1159/000162073. [DOI] [PubMed] [Google Scholar]
  5. Hart D. N., Newton M. R., Reece-Smith H., Fabre J. W., Morris P. J. Major histocompatibility complex antigens in the rat pancreas, isolated pancreatic islets, thyroid, and adrenal. Transplantation. 1983 Oct;36(4):431–435. doi: 10.1097/00007890-198310000-00015. [DOI] [PubMed] [Google Scholar]
  6. Medgyesi G. A., Miklós K., Kulics J., Füst G., Gergely J., Bazin H. Classes and subclasses of rat antibodies: reaction with the antigen and interaction of the complex with the complement system. Immunology. 1981 May;43(1):171–176. [PMC free article] [PubMed] [Google Scholar]
  7. Noble B., Yoshida T., Rose N. R., Bigazzi P. E. Thyroid antibodies in spontaneous autoimmune thyroiditis in the Buffalo rat. J Immunol. 1976 Nov;117(5 Pt 1):1447–1455. [PubMed] [Google Scholar]
  8. Penhale W. J., Farmer A., Urbaniak S. J., Irvine W. J. Susceptibility of inbred rat strains to experimental thyroiditis: quantitation of thyroglobulin-binding cells and assessment of T-cell function in susceptible and non-susceptible strains. Clin Exp Immunol. 1975 Jan;19(1):179–191. [PMC free article] [PubMed] [Google Scholar]
  9. Rennie D. P., McGregor A. M., Keast D., Weetman A. P., Foord S. M., Dieguez C., Williams E. D., Hall R. The influence of methimazole on thyroglobulin-induced autoimmune thyroiditis in the rat. Endocrinology. 1983 Jan;112(1):326–330. doi: 10.1210/endo-112-1-326. [DOI] [PubMed] [Google Scholar]
  10. Reuber M. D. Influence of age and sex on chronic thyroiditis in rats given subcutaneous injections of trypan blue. Toxicol Appl Pharmacol. 1970 Jul;17(1):60–66. doi: 10.1016/0041-008x(70)90131-6. [DOI] [PubMed] [Google Scholar]
  11. Silverman D. A., Rose N. R. Autoimmunity in methylcholanthrene-induced and spontaneous thyroiditis in Buffalo strain rats. Proc Soc Exp Biol Med. 1971 Nov;138(2):579–584. doi: 10.3181/00379727-138-35945. [DOI] [PubMed] [Google Scholar]
  12. Silverman D. A., Rose N. R. Neonatal thymectomy increases the incidence of spontaneous and methylcholanthrene-enhanced thyroiditis in rats. Science. 1974 Apr 12;184(4133):162–163. doi: 10.1126/science.184.4133.162. [DOI] [PubMed] [Google Scholar]
  13. Silverman D., Rose N. R. Spontaneous and methylcholanthrene-enhanced thyroiditis in BUF rats. II. Induction of experimental autoimmune thyroiditis without completed Freund's adjuvant. J Immunol. 1975 Jan;114(1 Pt 1):148–150. [PubMed] [Google Scholar]
  14. Spiegelberg H. L. Biological activities of immunoglobulins of different classes and subclasses. Adv Immunol. 1974;19(0):259–294. doi: 10.1016/s0065-2776(08)60254-0. [DOI] [PubMed] [Google Scholar]
  15. Taguchi O., Nishizuka Y. Self tolerance and localized autoimmunity. Mouse models of autoimmune disease that suggest tissue-specific suppressor T cells are involved in self tolerance. J Exp Med. 1987 Jan 1;165(1):146–156. doi: 10.1084/jem.165.1.146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Weetman A. P., Cohen S. The IgG subclass distribution of thyroid autoantibodies. Immunol Lett. 1986 Nov 3;13(6):335–341. doi: 10.1016/0165-2478(86)90068-4. [DOI] [PubMed] [Google Scholar]
  17. Weetman A. P., McGregor A. M. Autoimmune thyroid disease: developments in our understanding. Endocr Rev. 1984 Spring;5(2):309–355. doi: 10.1210/edrv-5-2-309. [DOI] [PubMed] [Google Scholar]
  18. Weetman A. P., McGregor A. M., Rennie D. P., Hall R. Sites of autoantibody production in rats with thyroiditis. Immunology. 1982 Jun;46(2):465–472. [PMC free article] [PubMed] [Google Scholar]

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