Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1986 Jul;65(1):112–119.

An antigenic study of human plasma cells in normal tissue and in myeloma: identification of a novel plasma cell associated antigen.

P D Nathan, L Walker, D Hardie, P Richardson, M Khan, G D Johnson, N R Ling
PMCID: PMC1542266  PMID: 3024883

Abstract

A mouse monoclonal antibody named BU11 which detects an antigen strongly expressed on human plasma cells is described. The antibody stains plasma cells in tonsil sections, fresh and cultured plasmacytoid cells from the bone marrow of patients with multiple myeloma and cells of the plasmacytoid cell line RPMI 8226 used as the immunogen. In vitro studies of pokeweed mitogen (PWM) stimulated peripheral blood B cells and Epstein-Barr virus (EBV) stimulated tonsil B cells show that the antigen is present mainly on cells coexpressing the OKT10 antigen and containing cytoplasmic immunoglobulin (cIg). The BU11 antigen is expressed weakly on some normal B cells and is not present on T cells, monocytes or granulocytes. The antigen is of molecular weight 58kD under reducing conditions and is biochemically distinct from previously described plasma cell antigens.

Full text

PDF
112

Images in this article

118Fig. 4
on p.118

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson K. C., Bates M. P., Slaughenhoupt B., Schlossman S. F., Nadler L. M. A monoclonal antibody with reactivity restricted to normal and neoplastic plasma cells. J Immunol. 1984 Jun;132(6):3172–3179. [PubMed] [Google Scholar]
  2. Anderson K. C., Boyd A. W., Fisher D. C., Leslie D., Schlossman S. F., Nadler L. M. Hairy cell leukemia: a tumor of pre-plasma cells. Blood. 1985 Mar;65(3):620–629. [PubMed] [Google Scholar]
  3. Anderson K. C., Park E. K., Bates M. P., Leonard R. C., Hardy R., Schlossman S. F., Nadler L. M. Antigens on human plasma cells identified by monoclonal antibodies. J Immunol. 1983 Mar;130(3):1132–1138. [PubMed] [Google Scholar]
  4. Freundlich B., Avdalovic N. Use of gelatin/plasma coated flasks for isolating human peripheral blood monocytes. J Immunol Methods. 1983 Aug 12;62(1):31–37. doi: 10.1016/0022-1759(83)90107-2. [DOI] [PubMed] [Google Scholar]
  5. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  6. Gordon J., Guy G., Walker L. Autocrine models of B-lymphocyte growth. I. Role of cell contact and soluble factors in T-independent B-cell responses. Immunology. 1985 Oct;56(2):329–335. [PMC free article] [PubMed] [Google Scholar]
  7. Kuritani T., Cooper M. D. Human b-cell differentiation. I. Analysis of immunoglobulin heavy chain switching using monoclonal anti-immunoglobulin M, G, and A antibodies and pokeweed mitogen-induced plasma cell differentiation. J Exp Med. 1982 Mar 1;155(3):839–851. doi: 10.1084/jem.155.3.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Ling N. R., Stephens G., Bratt P., Dhaliwal H. S. Attachment of antigens and antibodies to fixed red cells: their use in rosette and haemagllutination tests: a comparison with fresh red cells. Mol Immunol. 1979 Sep;16(9):637–642. doi: 10.1016/0161-5890(79)90002-6. [DOI] [PubMed] [Google Scholar]
  11. Mackenzie M. R., Paglieroni T. G. Plasma cell antigens in human multiple myeloma. J Lab Clin Med. 1977 May;89(5):1009–1017. [PubMed] [Google Scholar]
  12. Nadler L. M., Anderson K. C., Marti G., Bates M., Park E., Daley J. F., Schlossman S. F. B4, a human B lymphocyte-associated antigen expressed on normal, mitogen-activated, and malignant B lymphocytes. J Immunol. 1983 Jul;131(1):244–250. [PubMed] [Google Scholar]
  13. Saxon A., Feldhaus J., Robins R. A. Single step separation of human T and B cells using AET treated srbc rosettes. J Immunol Methods. 1976;12(3-4):285–288. doi: 10.1016/0022-1759(76)90050-8. [DOI] [PubMed] [Google Scholar]
  14. Stashenko P., Nadler L. M., Hardy R., Schlossman S. F. Characterization of a human B lymphocyte-specific antigen. J Immunol. 1980 Oct;125(4):1678–1685. [PubMed] [Google Scholar]
  15. Stashenko P., Nadler L. M., Hardy R., Schlossman S. F. Expression of cell surface markers after human B lymphocyte activation. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3848–3852. doi: 10.1073/pnas.78.6.3848. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Takahashi T., Old L. J., Hsu C. J., Boyse E. A. A new differentiation antigen of plasma cells. Eur J Immunol. 1971 Dec;1(6):478–482. doi: 10.1002/eji.1830010614. [DOI] [PubMed] [Google Scholar]
  17. Walker L., Johnson G. D., MacLennan I. C. The IgG subclass responses of human lymphocytes to B-cell activators. Immunology. 1983 Oct;50(2):269–272. [PMC free article] [PubMed] [Google Scholar]
  18. Watanabe T., Yagi Y., Pressman D. Antibody against neoplastic plasma cells. I. Specific surface antigens on mouse myeloma cells. J Immunol. 1971 May;106(5):1213–1221. [PubMed] [Google Scholar]
  19. Wu L. Y., Blanco A., Cooper M. D., Lawton A. R. Ontogeny of B-lymphocyte differentiation induced by pokeweed mitogen. Clin Immunol Immunopathol. 1976 Mar;5(2):208–217. doi: 10.1016/0090-1229(76)90026-x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES