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. 1986 Jul;65(1):57–65.

The relationship of soluble immune complexes, insulin antibodies and insulin-anti-insulin complexes to platelet and coagulation factors in type 1 diabetic patients with and without proliferative retinopathy.

U Di Mario, D Q Borsey, G Contreas, C V Prowse, B F Clarke, D Andreani
PMCID: PMC1542274  PMID: 2947762

Abstract

The possible correlation between soluble immune factors and platelet and coagulation factors has been evaluated in Type 1 diabetic patients with and without proliferative retinopathy, and in non-diabetic controls. Soluble immune complexes, platelet factor IV (PF4), beta-thromboglobulin, fibrinogen, factor VIII related antigen and anti-thrombin III were significantly increased in Type 1 diabetic patients with retinopathy as compared to non-diabetic controls. Fibrinogen and anti-thrombin III were also higher in those patients with retinopathy compared to those without retinopathy. A significant correlation was found between positive values of soluble immune complexes and increased levels of PF4 and beta-thromboglobulin in diabetic patients with retinopathy. The presence of soluble immune complexes and insulin-anti-insulin complexes was associated with a significantly greater number of elevated haemostatic factors in retinopathic patients. Our findings suggest that the interaction of platelets and soluble immune complexes or insulin-anti-insulin complexes may be pathologically relevant to the development of diabetic retinopathy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen O. O. Anti-insulin-antibodies and late diabetic complications. Acta Endocrinol (Copenh) 1976 Oct;83(2):329–340. doi: 10.1530/acta.0.0830329. [DOI] [PubMed] [Google Scholar]
  2. Andreani D., Di Mario U., Galfo C., Ventriglia L., Iavicoli M. Circulating immune complexes in diabetics with severe microangiopathy: evaluation by two different methods. Acta Endocrinol (Copenh) 1982 Feb;99(2):239–244. doi: 10.1530/acta.0.0990239. [DOI] [PubMed] [Google Scholar]
  3. Andreani D., Di Mario U., Zuccarini O., Franco M., Sensi M., Iavicoli M., Pozzilli P. Lymphocyte subsets and immune complexes in long-standing diabetic patients: relation with the presence of microangiopathy. Immunol Lett. 1984;8(1):17–21. doi: 10.1016/0165-2478(84)90098-1. [DOI] [PubMed] [Google Scholar]
  4. Andreani D. Malignant microangiopathy. Diabetologia. 1980 Mar;18(3):255–255. doi: 10.1007/BF00251926. [DOI] [PubMed] [Google Scholar]
  5. Bodansky H. J., Wolf E., Cudworth A. G., Dean B. M., Nineham L. J., Bottazzo G. F., Matthews J. A., Kurtz A. B., Kohner E. M. Genetic and immunologic factors in microvascular disease in type I insulin-dependent diabetes. Diabetes. 1982 Jan;31(1):70–74. doi: 10.2337/diab.31.1.70. [DOI] [PubMed] [Google Scholar]
  6. Bolton A. E., Ludlam C. A., Moore S., Pepper D. S., Cash J. D. Three approaches to the radioimmunoassay of human beta-thromboglobulin. Br J Haematol. 1976 Jun;33(2):233–238. doi: 10.1111/j.1365-2141.1976.tb03534.x. [DOI] [PubMed] [Google Scholar]
  7. Bolton A. E., Ludlam C. A., Pepper D. S., Moore S., Cash J. D. A radioimmunoassay for platelet factor 4. Thromb Res. 1976 Jan;8(1):51–58. doi: 10.1016/0049-3848(76)90122-5. [DOI] [PubMed] [Google Scholar]
  8. Borsey D. Q., Dawes J., Fraser D. M., Prowse C. V., Elton R. A., Clarke B. F. Plasma beta-thromboglobulin in diabetes mellitus. Diabetologia. 1980 May;18(5):353–357. doi: 10.1007/BF00276813. [DOI] [PubMed] [Google Scholar]
  9. Campbell I. W., Dawes J., Fraser D. M., Pepper D. S., Clarke B. F., Duncan L. J., Cash J. D. Plasma beta-thromboglobulin in diabetes mellitus. Diabetes. 1977 Dec;26(12):1175–1177. doi: 10.2337/diab.26.12.1175. [DOI] [PubMed] [Google Scholar]
  10. Casali P., Bossus A., Carpentier N. A., Lambert P. H. Solid-phase enzyme immunoassay or radioimmunoassay for the detection of immune complexes based on their recognition by conglutinin: conglutinin-binding test. A comparative study with 125I-labelled Clq binding and Raji-cell RIA tests. Clin Exp Immunol. 1977 Aug;29(2):342–354. [PMC free article] [PubMed] [Google Scholar]
  11. Di Mario U., Fallucca F., Gargiulo P., Tiberti C., Scardellato A., Arduini P., Pachi' A., Andreani D. Insulin-anti-insulin complexes in diabetic women and their neonates. Diabetologia. 1984 Jul;27 (Suppl):83–86. doi: 10.1007/BF00275654. [DOI] [PubMed] [Google Scholar]
  12. Di Mario U., Guy K., Irvine W. J. The detection of circulating immune complexes in normal subjects using four different methods. J Clin Lab Immunol. 1981 Mar;5(2):95–99. [PubMed] [Google Scholar]
  13. Di Mario U., Ventriglia L., Iavicoli M., Guy K., Andreani D. The correlation between insulin antibodies and circulating immune complexes in diabetics with and without microangiopathy. Clin Exp Immunol. 1983 Jun;52(3):575–580. [PMC free article] [PubMed] [Google Scholar]
  14. ELLIS B. C., STRANSKY A. A quick and accurate method for the determination of fibronogen in plasma. J Lab Clin Med. 1961 Sep;58:477–488. [PubMed] [Google Scholar]
  15. Folling I. Insulin--anti-insulin complexes. Acta Endocrinol Suppl (Copenh) 1976;205:199–209. [PubMed] [Google Scholar]
  16. Fuller J. H., Keen H., Jarrett R. J., Omer T., Meade T. W., Chakrabarti R., North W. R., Stirling Y. Haemostatic variables associated with diabetes and its complications. Br Med J. 1979 Oct 20;2(6196):964–966. doi: 10.1136/bmj.2.6196.964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Iavicoli M., Di Mario U., Pozzilli P., Canalese J., Ventriglia L., Galfo C., Andreani D. Impaired phagocytic function and increased immune complexes in diabetics with severe microangiopathy. Diabetes. 1982 Jan;31(1):7–11. doi: 10.2337/diab.31.1.7. [DOI] [PubMed] [Google Scholar]
  18. Irvine W. J., Al-Khateeb S. F., Di Mario U., Feek C. M., Gray R. S., Edmond B., Duncan L. J. Soluble immune complexes in the sera of newly diagnosed insulin-dependent diabetics and in treated diabetics. Clin Exp Immunol. 1977 Oct;30(1):16–21. [PMC free article] [PubMed] [Google Scholar]
  19. Irvine W. J., Di Mario U., Guy K., Iavicoli M., Pozzilli P., Lumbroso B., Andreani D. Immune complexes and diabetic microangiopathy. J Clin Lab Immunol. 1978 Nov;1(3):187–191. [PubMed] [Google Scholar]
  20. Jones R. L., Peterson C. M. Hematologic alterations in diabetes mellitus. Am J Med. 1981 Feb;70(2):339–352. doi: 10.1016/0002-9343(81)90771-3. [DOI] [PubMed] [Google Scholar]
  21. Kaplan K. L., Owen J. Plasma levels of beta-thromboglobulin and platelet factor 4 as indices of platelet activation in vivo. Blood. 1981 Feb;57(2):199–202. [PubMed] [Google Scholar]
  22. Kilpatrick J. M., Virella G. Isolation and characterization of soluble insulin-anti-insulin immune complexes formed in vitro and in vivo in sera from patients with diabetes mellitus. Clin Exp Immunol. 1980 Jun;40(3):445–452. [PMC free article] [PubMed] [Google Scholar]
  23. Kockum C. Radioimmunoassay of fibrinopeptide A-clinical applications. Thromb Res. 1976 Feb;8(2):225–236. doi: 10.1016/0049-3848(76)90265-6. [DOI] [PubMed] [Google Scholar]
  24. Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
  25. MOHOS S. C., HENNIGAR G. R., FOGELMAN J. A. INSULIN-INDUCED GLOMERULOSCLEROSIS IN THE RABBIT. J Exp Med. 1963 Nov 1;118:667–680. doi: 10.1084/jem.118.5.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Preston F. E., Ward J. D., Marcola B. H., Porter N. R., Timperley W. R., O'Malley B. C. Elevated beta-thromboglobulin levels and circulating platelet aggregates in diabetic microangiopathy. Lancet. 1978 Feb 4;1(8058):238–240. doi: 10.1016/s0140-6736(78)90483-x. [DOI] [PubMed] [Google Scholar]
  27. Virella G., Russell D., Laimins M., Colwell J. Simplified method for detecting anti-insulin antibodies and insulin-anti-insulin immune complexes. Clin Chem. 1980 Aug;26(9):1357–1359. [PubMed] [Google Scholar]
  28. Virella G., Wohltmann H., Sagel J., Lopes-Virella M. F., Kilpatrick M., Phillips C., Colwell J. Soluble immune complexes in patients with diabetes mellitus: detection and pathological significance. Diabetologia. 1981 Sep;21(3):184–191. doi: 10.1007/BF00252652. [DOI] [PubMed] [Google Scholar]
  29. Westberg N. G., Michael A. F. Immunohistopathology of diabetic glomerulosclerosis. Diabetes. 1972 Mar;21(3):163–174. doi: 10.2337/diab.21.3.163. [DOI] [PubMed] [Google Scholar]

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