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. 1986 Jul;65(1):190–197.

Regulation of immune interferon production during the response to soluble microbial antigens.

E Piccolella, A Dolei, G Lombardi, D Vismara, P Pizzoli, V Colizzi, F Dianzani
PMCID: PMC1542282  PMID: 2431816

Abstract

Human peripheral blood mononuclear cells (PBMC) stimulated in vitro with purified protein derivative (PPD) or with a Candida albicans polysaccharide extract (MPPS) released immune interferon (IFN) and interleukin 2 (IL-2). Kinetic studies showed a biphasic production of IFN with maximum levels at days 3-4 and days 5-6 of culture. In contrast, the IL-2 production is only observed at days 2-3 of culture. The relationship between IFN and IL-2, analysed both in responder and nonresponder PBMC cultures, showed that the early peak of IFN production appears to be IL-2 independent whereas the second peak seems strictly related to the presence of IL-2 culture. Furthermore, monoclonal antibodies against class I and class II products of the major histocompatibility complex (MHC) inhibited IFN production when added at the beginning of culture, whereas only anti-class I antibodies interfered with the release of IFN when added to antigen-primed lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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