Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1986 May;64(2):432–441.

Immunoglobulin- and J chain-producing cells associated with lymphoid follicles in the human appendix, colon and ileum, including Peyer's patches.

K Bjerke, P Brandtzaeg
PMCID: PMC1542337  PMID: 3091305

Abstract

Immunohistochemistry of B cells associated with normal human Peyer's patches and solitary lymphoid follicles of the ileum, colon and appendix mucosa showed that local accumulation of IgG-producing cells is a common feature of gut-associated lymphoid tissue (GALT). These immunocytes have strikingly down-regulated J-chain expression, indicating that they belong to mature memory clones. They are located mainly in the dome areas, alongside the follicles, and to a lesser extent in the germinal centres, and are accompanied by a much smaller number of J-chain negative IgA- and IgM-producing cells. It is concluded that B cells of mature memory clones are retained in GALT, whereas relatively early counterparts with a high J chain-expressing potential probably emigrate rapidly after stimulation and seed distant secretory sites where they undergo terminal differentiation to produce mainly J chain-containing dimeric IgA.

Full text

PDF
432

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRIDGES R. A., CONDIE R. M., ZAK S. J., GOOD R. A. The morphologic basis of antibody formation development during the neonatal period. J Lab Clin Med. 1959 Mar;53(3):331–357. [PubMed] [Google Scholar]
  2. Bienenstock J., Dolezel J. Peyer's patches: lack of specific antibody-containing cells after oral and parenteral immunization. J Immunol. 1971 Apr;106(4):938–945. [PubMed] [Google Scholar]
  3. Brandtzaeg P. Immunohistochemical characterization of intracellular J-chain and binding site for secretory component (SC) in human immunoglobulin (Ig)-producing cells. Mol Immunol. 1983 Sep;20(9):941–966. doi: 10.1016/0161-5890(83)90036-6. [DOI] [PubMed] [Google Scholar]
  4. Brandtzaeg P., Korsrud F. R. Significance of different J chain profiles in human tissues: generation of IgA and IgM with binding site for secretory component is related to the J chain expressing capacity of the total local immunocyte population, including IgG and IgD producing cells, and depends on the clinical state of the tissue. Clin Exp Immunol. 1984 Dec;58(3):709–718. [PMC free article] [PubMed] [Google Scholar]
  5. Brandtzaeg P. Prolonged incubation time in immunohistochemistry: effects on fluorescence staining of immunoglobulins and epithelial components in ethanol- and formaldehyde-fixed paraffin-embedded tissues. J Histochem Cytochem. 1981 Nov;29(11):1302–1315. doi: 10.1177/29.11.7033362. [DOI] [PubMed] [Google Scholar]
  6. Brandtzaeg P. Role of J chain and secretory component in receptor-mediated glandular and hepatic transport of immunoglobulins in man. Scand J Immunol. 1985 Aug;22(2):111–146. doi: 10.1111/j.1365-3083.1985.tb01866.x. [DOI] [PubMed] [Google Scholar]
  7. Brandtzaeg P., Valnes K., Scott H., Rognum T. O., Bjerke K., Baklien K. The human gastrointestinal secretory immune system in health and disease. Scand J Gastroenterol Suppl. 1985;114:17–38. doi: 10.3109/00365528509093765. [DOI] [PubMed] [Google Scholar]
  8. Butcher E. C., Rouse R. V., Coffman R. L., Nottenburg C. N., Hardy R. R., Weissman I. L. Surface phenotype of Peyer's patch germinal center cells: implications for the role of germinal centers in B cell differentiation. J Immunol. 1982 Dec;129(6):2698–2707. [PubMed] [Google Scholar]
  9. Cornes J. S. Number, size, and distribution of Peyer's patches in the human small intestine: Part I The development of Peyer's patches. Gut. 1965 Jun;6(3):225–229. doi: 10.1136/gut.6.3.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Crabbé P. A., Nash D. R., Bazin H., Eyssen H., Heremans J. F. Immunohistochemical observations on lymphoid tissues from conventional and germ-free mice. Lab Invest. 1970 May;22(5):448–457. [PubMed] [Google Scholar]
  11. Craig S. W., Cebra J. J. Peyer's patches: an enriched source of precursors for IgA-producing immunocytes in the rabbit. J Exp Med. 1971 Jul 1;134(1):188–200. doi: 10.1084/jem.134.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Elson C. O., Ealding W. Generalized systemic and mucosal immunity in mice after mucosal stimulation with cholera toxin. J Immunol. 1984 Jun;132(6):2736–2741. [PubMed] [Google Scholar]
  13. Elson C. O. Induction and control of the gastrointestinal immune system. Scand J Gastroenterol Suppl. 1985;114:1–15. doi: 10.3109/00365528509093764. [DOI] [PubMed] [Google Scholar]
  14. Hamilton S. R., Keren D. F., Yardley J. H., Brown G. No impairment of local intestinal immune response to keyhole limpet haemocyanin in the absence of Peyer's patches. Immunology. 1981 Mar;42(3):431–435. [PMC free article] [PubMed] [Google Scholar]
  15. Husband A. J., Gowans J. L. The origin and antigen-dependent distribution of IgA-containing cells in the intestine. J Exp Med. 1978 Nov 1;148(5):1146–1160. doi: 10.1084/jem.148.5.1146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Husband A. J. Kinetics of extravasation and redistribution of IgA-specific antibody-containing cells in the intestine. J Immunol. 1982 Mar;128(3):1355–1359. [PubMed] [Google Scholar]
  17. Kagnoff M. F., Campbell S. Functional characteristics of Peyer's patch lymphoid cells. I. Induction of humoral antibody and cell-mediated allograft reactions. J Exp Med. 1974 Feb 1;139(2):398–406. doi: 10.1084/jem.139.2.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Korsrud F. R., Brandtzaeg P. Immunohistochemical evaluation of J-chain expression by intra- and extra-follicular immunoglobulin-producing human tonsillar cells. Scand J Immunol. 1981;13(3):271–280. doi: 10.1111/j.1365-3083.1981.tb00135.x. [DOI] [PubMed] [Google Scholar]
  19. Kraal G., Weissman I. L., Butcher E. C. Germinal centre B cells: antigen specificity and changes in heavy chain class expression. Nature. 1982 Jul 22;298(5872):377–379. doi: 10.1038/298377a0. [DOI] [PubMed] [Google Scholar]
  20. Layton G. T., Smithyman A. M. The optimum conditions for antibody production in vitro by Peyer's patch lymphocytes and a comparison between total immunoglobulins and antigen (TNP)-specific antibody synthesis following mitogen stimulation. Int Arch Allergy Appl Immunol. 1983;72(2):158–163. doi: 10.1159/000234859. [DOI] [PubMed] [Google Scholar]
  21. Lycke N., Lindholm L., Holmgren J. IgA isotype restriction in the mucosal but not in the extramucosal immune response after oral immunizations with cholera toxin or cholera B subunit. Int Arch Allergy Appl Immunol. 1983;72(2):119–127. doi: 10.1159/000234853. [DOI] [PubMed] [Google Scholar]
  22. McClelland D. B. Peyer's-patch-associated synthesis of immunoglobulin in germ-free, specific-pathogen-free, and conventional mice. Scand J Immunol. 1976;5(8):909–915. doi: 10.1111/j.1365-3083.1976.tb03041.x. [DOI] [PubMed] [Google Scholar]
  23. Nieuwenhuis P., van Nouhuijs C. E., Eggens J. H., Keuning F. J. Germinal centres and the origin of the B-cell system. I. Germinal centres in the rabbit appendix. Immunology. 1974 Mar;26(3):497–507. [PMC free article] [PubMed] [Google Scholar]
  24. Pierce N. F., Cray W. C., Jr Determinants of the localization, magnitude, and duration of a specific mucosal IgA plasma cell response in enterically immunized rats. J Immunol. 1982 Mar;128(3):1311–1315. [PubMed] [Google Scholar]
  25. Rosner A. J., Keren D. F. Demonstration of M cells in the specialized follicle-associated epithelium overlying isolated lymphoid follicles in the gut. J Leukoc Biol. 1984 Apr;35(4):397–404. doi: 10.1002/jlb.35.4.397. [DOI] [PubMed] [Google Scholar]
  26. Rudzik R., Clancy R. L., Perey D. Y., Day R. P., Bienenstock J. Repopulation with IgA-containing cells of bronchial and intestinal lamina propria after transfer of homologous Peyer's patch and bronchial lymphocytes. J Immunol. 1975 May;114(5):1599–1604. [PubMed] [Google Scholar]
  27. Sminia T., Delemarre F., Janse E. M. Histological observations on the intestinal immune response towards horseradish peroxidase in rats. Immunology. 1983 Sep;50(1):53–56. [PMC free article] [PubMed] [Google Scholar]
  28. Sminia T., Plesch B. E. An immunohistochemical study of cells with surface and cytoplasmic immunoglobulins in situ in Peyer's patches and lamina propria of rat small intestine. Virchows Arch B Cell Pathol Incl Mol Pathol. 1982 Aug;40(2):181–189. doi: 10.1007/BF02932863. [DOI] [PubMed] [Google Scholar]
  29. Tseng J. Repopulation of the gut lamina propria with IgA-containing cells by lymphoid cells isolated from the gut lamina propria. Eur J Immunol. 1984 May;14(5):420–425. doi: 10.1002/eji.1830140507. [DOI] [PubMed] [Google Scholar]
  30. Valnes K., Brandtzaeg P. Retardation of immunofluorescence fading during microscopy. J Histochem Cytochem. 1985 Aug;33(8):755–761. doi: 10.1177/33.8.3926864. [DOI] [PubMed] [Google Scholar]
  31. Veldkamp J., van der Gaag R., Willers J. M. The role of Peyer's patch cells in antibody formation. Immunology. 1973 Nov;25(5):761–771. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES