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. 1987 Sep;69(3):611–617.

Human T cell proliferative responses to particulate microbial antigens are supported by populations enriched in dendritic cells.

A Mittal 1, I Nath 1
PMCID: PMC1542363  PMID: 3311498

Abstract

The efficacy of dendritic cells in antigen presentation was studied in eight healthy subjects using a lymphoproliferation assay. Both particulate (Mycobacterium leprae, H37Ra) and soluble (PPD, tetanus toxoid) bacterial antigens were used as stimulants over a concentration range of accessory cells (monocytes (MO) and dendritic cells (DC)) varying from 10 to 0.1% in co-cultures using T-enriched cells. In general, co-cultures with T + MO and T + DC at all concentrations of accessory cells showed significant improvement of antigen-induced lymphoproliferation over PBMC cultures. The improvement in delta ct/min of T + DC co-cultures as compared to T + MO with parallel concentrations of accessory cells (P less than 0.05 to less than 0.01) was significant. Of the bacterial antigens used to test the antigen-presenting ability of DC, the particulate antigen (H37Ra) showed the most impressive improvement (380%) of T cell proliferation in DC reconstituted cultures as compared to monocytes. PPD, soluble protein derived from a similar tuberculosis strain of mycobacteria, was not presented as effectively as the particulate equivalent even though the donors of the appropriate cell combinations showed skin test reactivity to this antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crow M. K., Kunkel H. G. Human dendritic cells: major stimulators of the autologous and allogeneic mixed leucocyte reactions. Clin Exp Immunol. 1982 Aug;49(2):338–346. [PMC free article] [PubMed] [Google Scholar]
  2. Kaye P. M., Chain B. M., Feldmann M. Nonphagocytic dendritic cells are effective accessory cells for anti-mycobacterial responses in vitro. J Immunol. 1985 Mar;134(3):1930–1934. [PubMed] [Google Scholar]
  3. Klinkert W. E., LaBadie J. H., O'Brien J. P., Beyer C. F., Bowers W. E. Rat dendritic cells function as accessory cells and control the production of a soluble factor required for mitogenic responses of T lymphocytes. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5414–5418. doi: 10.1073/pnas.77.9.5414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Mason D. W., Pugh C. W., Webb M. The rat mixed lymphocyte reaction: roles of a dendritic cell in intestinal lymph and T-cell subsets defined by monoclonal antibodies. Immunology. 1981 Sep;44(1):75–87. [PMC free article] [PubMed] [Google Scholar]
  5. Nath I., Sathish M., Jayaraman T., Bhutani L. K., Sharma A. K. Evidence for the presence of M. leprae reactive T lymphocytes in patients with lepromatous leprosy. Clin Exp Immunol. 1984 Dec;58(3):522–530. [PMC free article] [PubMed] [Google Scholar]
  6. Nussenzweig M. C., Steinman R. M., Witmer M. D., Gutchinov B. A monoclonal antibody specific for mouse dendritic cells. Proc Natl Acad Sci U S A. 1982 Jan;79(1):161–165. doi: 10.1073/pnas.79.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Prasad H. K., Nath I. Incorporation of 3H-thymidine in Mycobacterium leprae within differentiated human macrophages. J Med Microbiol. 1981 Aug;14(3):279–293. doi: 10.1099/00222615-14-3-279. [DOI] [PubMed] [Google Scholar]
  8. Sathish M., Bhutani L. K., Sharma A. K., Nath I. Monocyte-derived soluble suppressor factor(s) in patients with lepromatous leprosy. Infect Immun. 1983 Dec;42(3):890–899. doi: 10.1128/iai.42.3.890-899.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Steinman R. M., Cohn Z. A. Identification of a novel cell type in peripheral lymphoid organs of mice. I. Morphology, quantitation, tissue distribution. J Exp Med. 1973 May 1;137(5):1142–1162. doi: 10.1084/jem.137.5.1142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Steinman R. M., Kaplan G., Witmer M. D., Cohn Z. A. Identification of a novel cell type in peripheral lymphoid organs of mice. V. Purification of spleen dendritic cells, new surface markers, and maintenance in vitro. J Exp Med. 1979 Jan 1;149(1):1–16. doi: 10.1084/jem.149.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sunshine G. H., Gold D. P., Wortis H. H., Marrack P., Kappler J. W. Mouse spleen dendritic cells present soluble antigens to antigen-specific T cell hybridomas. J Exp Med. 1983 Nov 1;158(5):1745–1750. doi: 10.1084/jem.158.5.1745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Van Voorhis W. C., Hair L. S., Steinman R. M., Kaplan G. Human dendritic cells. Enrichment and characterization from peripheral blood. J Exp Med. 1982 Apr 1;155(4):1172–1187. doi: 10.1084/jem.155.4.1172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Van Voorhis W. C., Steinman R. M., Hair L. S., Luban J., Witmer M. D., Koide S., Cohn Z. A. Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages. J Exp Med. 1983 Jul 1;158(1):126–145. doi: 10.1084/jem.158.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]

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