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. 1987 Aug;69(2):426–432.

Multiple myeloma: ecto-5' nucleotidase deficiency of suppressor/cytotoxic (CD8) lymphocytes is a marker for the expansion of suppressor T cells.

M Massaia 1, U Dianzani 1, P Pioppo 1, E Sibilla 1, M Boccadoro 1, A Pileri 1
PMCID: PMC1542394  PMID: 2820641

Abstract

In this study, 5'NT activity was investigated by radiochemical and cytochemical assays in T cell subpopulations of patients with multiple myeloma (MM). Cytotoxic/suppressor (CD8) and helper/inducer (CD4) lymphocytes were separated from peripheral blood by the panning technique, or sorted with a fluorescein activated cell sorter. 5'NT activity was significantly decreased in MM CD8 lymphocytes compared with the controls. By contrast, it was comparably low in CD4 subpopulations of normal controls and MM patients. The cytochemical assay indicated that a decreased number of 5'NT+ cells rather than a decreased activity per cell was responsible for 5'NT deficiency in MM CD8 lymphocytes. CD8 lymphocytes with the suppressor phenotype (OKMI+, granular cells) were significantly increased in MM. These data provide a phenotypic explanation for the enhanced suppressor activity displayed by T lymphocytes in MM. A significant correlation was found between the increase of suppressor cells and the decrease of 5'NT+ cells. 5'NT deficiency of CD8 lymphocytes can be a biochemical marker for the expansion of suppressor T cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Broder S., Humphrey R., Durm M., Blackman M., Meade B., Goldman C., Strober W., Waldmann T. Impaired synthesis of polyclonal (non-paraprotein) immunoglobulins by circulating lymphocytes from patients with multiple myeloma Role of suppressor cells. N Engl J Med. 1975 Oct 30;293(18):887–892. doi: 10.1056/NEJM197510302931801. [DOI] [PubMed] [Google Scholar]
  2. Clement L. T., Grossi C. E., Gartland G. L. Morphologic and phenotypic features of the subpopulation of Leu-2+ cells that suppresses B cell differentiation. J Immunol. 1984 Nov;133(5):2461–2468. [PubMed] [Google Scholar]
  3. Dianzani U., Massaia M., Pileri A., Grossi C. E., Clement L. T. Differential expression of ecto-5' nucleotidase activity by functionally and phenotypically distinct subpopulations of human Leu-2+/T8+ lymphocytes. J Immunol. 1986 Jul 15;137(2):484–489. [PubMed] [Google Scholar]
  4. Edwards N. L., Gelfand E. W., Burk L., Dosch H. M., Fox I. H. Distribution of 5'-nucleotidase in human lymphoid tissues. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3474–3476. doi: 10.1073/pnas.76.7.3474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Greaves M. F., Brown G. Purification of human T and B lymphocytes. J Immunol. 1974 Jan;112(1):420–423. [PubMed] [Google Scholar]
  6. Hoover R. G., Hickman S., Gebel H. M., Rebbe N., Lynch R. G. Expansion of Fc receptor-bearing T lymphocytes in patients with immunoglobulin G and immunoglobulin A myeloma. J Clin Invest. 1981 Jan;67(1):308–311. doi: 10.1172/JCI110028. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Landay A., Clement L. T., Grossi C. E. Phenotypically and functionally distinct subpopulations of human lymphocytes with T cell markers also exhibit different cytochemical patterns of staining for lysosomal enzymes. Blood. 1984 May;63(5):1067–1071. [PubMed] [Google Scholar]
  8. Landay A., Gartland G. L., Clement L. T. Characterization of a phenotypically distinct subpopulation of Leu-2+ cells that suppresses T cell proliferative responses. J Immunol. 1983 Dec;131(6):2757–2761. [PubMed] [Google Scholar]
  9. Lauria F., Foa R., Cavo M., Gobbi M., Raspadori D., Giubellino M. C., Tazzari P. L., Tura S. Membrane phenotype and functional behaviour of T lymphocytes in multiple myeloma: correlation with clinical stages of the disease. Clin Exp Immunol. 1984 Jun;56(3):653–658. [PMC free article] [PubMed] [Google Scholar]
  10. Massaia M., Ma D. D., Boccadoro M., Golzio F., Gavarotti P., Dianzani U., Pileri A. Decreased ecto-5'nucleotidase activity of peripheral blood lymphocytes in human monoclonal gammopathies: correlation with tumor cell kinetics. Blood. 1985 Mar;65(3):530–534. [PubMed] [Google Scholar]
  11. Massaia M., Ma D. D., Sylwestrowicz T. A., Tidman N., Price G., Janossy G., Hoffbrand A. V. Enzymes of purine metabolism in human peripheral lymphocyte subpopulations. Clin Exp Immunol. 1982 Oct;50(1):148–154. [PMC free article] [PubMed] [Google Scholar]
  12. Mills K. H., Cawley J. C. Abnormal monoclonal antibody-defined helper/suppressor T-cell subpopulations in multiple myeloma: relationship to treatment and clinical stage. Br J Haematol. 1983 Feb;53(2):271–275. doi: 10.1111/j.1365-2141.1983.tb02021.x. [DOI] [PubMed] [Google Scholar]
  13. Murray J. L., Reuben J. M., Munn C. G., Mansell P. W., Newell G. R., Hersh E. M. Decreased 5' nucleotidase activity in lymphocytes from asymptomatic sexually active homosexual men and patients with the acquired immune deficiency syndrome. Blood. 1984 Nov;64(5):1016–1021. [PubMed] [Google Scholar]
  14. Paglieroni T., Mackenzie M. R. Multiple myeloma: an immunologic profile. Cytotoxic and suppressive effects of the EA rosette-forming cell. J Immunol. 1980 Jun;124(6):2563–2570. [PubMed] [Google Scholar]
  15. Pellegrino M. A., Ferrone S., Dierich M. P., Reisfeld R. A. Enhancement of sheep red blood cell human lymphocyte rosette formation by the sulfhydryl compound 2-amino ethylisothiouronium bromide. Clin Immunol Immunopathol. 1975 Jan;3(3):324–333. doi: 10.1016/0090-1229(75)90019-7. [DOI] [PubMed] [Google Scholar]
  16. Perri R. T., Oken M. M., Kay N. E. Enhanced T cell suppression is directed toward sensitive circulating B cells in multiple myeloma. J Lab Clin Med. 1982 Apr;99(4):512–519. [PubMed] [Google Scholar]
  17. Platsoucas C. D., Hansen H. J., Redman J. R., Berenson S., Lee B. J., 3rd, Clarkson B. D. T-cell imbalances in patients with multiple myeloma: an analysis by monoclonal antibodies. J Clin Immunol. 1983 Jul;3(3):277–284. doi: 10.1007/BF00915352. [DOI] [PubMed] [Google Scholar]
  18. Salazar-Gonzalez J. F., Moody D. J., Giorgi J. V., Martinez-Maza O., Mitsuyasu R. T., Fahey J. L. Reduced ecto-5'-nucleotidase activity and enhanced OKT10 and HLA-DR expression on CD8 (T suppressor/cytotoxic) lymphocytes in the acquired immune deficiency syndrome: evidence of CD8 cell immaturity. J Immunol. 1985 Sep;135(3):1778–1785. [PubMed] [Google Scholar]
  19. Silber R., Conklyn M. Decreased 5'-nucleotidase activity in a T lymphocyte subpopulation from patients with B cell chronic lymphocytic leukemia. Blood. 1984 Aug;64(2):479–481. [PubMed] [Google Scholar]
  20. Silber R., Conklyn M., Grusky G., Zucker-Franklin D. Human lymphocytes: 5'-nucleotidase-positive and -negative subpopulations. J Clin Invest. 1975 Nov;56(5):1324–1327. doi: 10.1172/JCI108209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sylwestrowicz T. A., Ma D. D., Murphy P. P., Massaia M., Prentice H. G., Hoffbrand A. V., Greaves M. F. 5'nucleotidase, adenosine deaminase and purine nucleoside phosphorylase activities in acute leukaemia. Leuk Res. 1982;6(4):475–482. doi: 10.1016/0145-2126(82)90004-2. [DOI] [PubMed] [Google Scholar]
  22. Thompson L. F., Saxon A., O'Connor R. D., Fox R. I. Ecto-5'-nucleotidase activity in human T cell subsets. Decreased numbers of ecto-5'-nucleotidase positive cells from both OKT4+ and OKT8+ cells in patients with hypogammaglobulinemia. J Clin Invest. 1983 Apr;71(4):892–899. doi: 10.1172/JCI110843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wysocki L. J., Sato V. L. "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844–2848. doi: 10.1073/pnas.75.6.2844. [DOI] [PMC free article] [PubMed] [Google Scholar]

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