Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1987 Aug;69(2):308–313.

Insulin autoantibodies, islet cell surface antibodies and the development of spontaneous diabetes in the BB/Edinburgh rat.

B M Dean 1, A J Bone 1, A M Varey 1, R Walker 1, J D Baird 1, A Cooke 1
PMCID: PMC1542413  PMID: 3308226

Abstract

The presence of insulin autoantibodies (IAA) and islet cell surface antibodies (ICSA) was sought in two longitudinal studies, involving BB/Edinburgh rats of high (BB/E/H, n = 157) and low (BB/E/L, n = 61) susceptibility to diabetes development. Both studies were designed to correlate pancreatic morphology with cellular and humoral immunity. In Study I, groups of eight male and eight female non-diabetic rats of the BB/E/H line were killed at 15 day intervals from 30-105 days and plasma samples were obtained by cardiac puncture. In study II, 61 BB/E/H and 41 BB/E/L rats underwent pancreatic biopsy 1-3 times from 30 days of age until onset of diabetes or 150 days, plasma samples being taken from the tail vein at biopsy. Both studies revealed a higher prevalence for ICSA than IAA in BB/E rats. Whereas a highly significant association of ICSA with diabetes development was observed in study II (chi 2 = 8.30, P less than 0.005), IAA were associated with diabetes development only weakly (P less than 0.03, Mann-Witney U-rank test). No correlation between the presence of ICSA and IAA in individual rats was observed and IAA were not significantly associated with BB/E/H in preference to BB/E/L rats, although positive IAA values were significantly elevated in the former compared with the latter (P less than 0.01). These observations support the concept that IAA form part of a background of heightened autoimmunity against which frank diabetes develops in some animals.

Full text

PDF
308

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arslanian S. A., Becker D. J., Rabin B., Atchison R., Eberhardt M., Cavender D., Dorman J., Drash A. L. Correlates of insulin antibodies in newly diagnosed children with insulin-dependent diabetes before insulin therapy. Diabetes. 1985 Sep;34(9):926–930. doi: 10.2337/diab.34.9.926. [DOI] [PubMed] [Google Scholar]
  2. Atkinson M. A., Maclaren N. K., Riley W. J., Winter W. E., Fisk D. D., Spillar R. P. Are insulin autoantibodies markers for insulin-dependent diabetes mellitus? Diabetes. 1986 Aug;35(8):894–898. doi: 10.2337/diab.35.8.894. [DOI] [PubMed] [Google Scholar]
  3. Bodansky H. J., Grant P. J., Dean B. M., McNally J., Bottazzo G. F., Hambling M. H., Wales J. K. Islet-cell antibodies and insulin autoantibodies in association with common viral infections. Lancet. 1986 Dec 13;2(8520):1351–1353. doi: 10.1016/s0140-6736(86)92003-9. [DOI] [PubMed] [Google Scholar]
  4. Bone A. J., Swenne I. Microcarriers: a new approach to pancreatic islet cell culture. In Vitro. 1982 Feb;18(2):141–148. doi: 10.1007/BF02796406. [DOI] [PubMed] [Google Scholar]
  5. Cooke A., Lydyard P. M., Roitt I. M. Autoimmunity and idiotypes. Lancet. 1984 Sep 29;2(8405):723–725. doi: 10.1016/s0140-6736(84)92628-x. [DOI] [PubMed] [Google Scholar]
  6. Dean B. M., Becker F., McNally J. M., Tarn A. C., Schwartz G., Gale E. A., Bottazzo G. F. Insulin autoantibodies in the pre-diabetic period: correlation with islet cell antibodies and development of diabetes. Diabetologia. 1986 May;29(5):339–342. doi: 10.1007/BF00452073. [DOI] [PubMed] [Google Scholar]
  7. Dyrberg T., Nakhooda A. F., Baekkeskov S., Lernmark A., Poussier P., Marliss E. B. Islet cell surface antibodies and lymphocyte antibodies in the spontaneously diabetic BB Wistar rat. Diabetes. 1982 Mar;31(3):278–281. doi: 10.2337/diab.31.3.278. [DOI] [PubMed] [Google Scholar]
  8. Elder M., Maclaren N., Riley W., McConnell T. Gastric parietal cell and other autoantibodies in the BB rat. Diabetes. 1982 Apr;31(4 Pt 1):313–318. doi: 10.2337/diab.31.4.313. [DOI] [PubMed] [Google Scholar]
  9. Karjalainen J., Knip M., Mustonen A., Ilonen J., Akerblom H. K. Relation between insulin antibody and complement-fixing islet cell antibody at clinical diagnosis of IDDM. Diabetes. 1986 May;35(5):620–622. doi: 10.2337/diab.35.5.620. [DOI] [PubMed] [Google Scholar]
  10. Nakhooda A. F., Like A. A., Chappel C. I., Murray F. T., Marliss E. B. The spontaneously diabetic Wistar rat. Metabolic and morphologic studies. Diabetes. 1977 Feb;26(2):100–112. doi: 10.2337/diab.26.2.100. [DOI] [PubMed] [Google Scholar]
  11. Palmer J. P., Asplin C. M., Clemons P., Lyen K., Tatpati O., Raghu P. K., Paquette T. L. Insulin antibodies in insulin-dependent diabetics before insulin treatment. Science. 1983 Dec 23;222(4630):1337–1339. doi: 10.1126/science.6362005. [DOI] [PubMed] [Google Scholar]
  12. Srikanta S., Ricker A. T., McCulloch D. K., Soeldner J. S., Eisenbarth G. S., Palmer J. P. Autoimmunity to insulin, beta cell dysfunction, and development of insulin-dependent diabetes mellitus. Diabetes. 1986 Feb;35(2):139–142. doi: 10.2337/diab.35.2.139. [DOI] [PubMed] [Google Scholar]
  13. WEIGLE W. O. THE INDUCTION OF AUTOIMMUNITY IN RABBITS FOLLOWING INJECTION OF HETEROLOGOUS OR ALTERED HOMOLOGOUS THYROGLOBULIN. J Exp Med. 1965 Feb 1;121:289–308. doi: 10.1084/jem.121.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wassermann N. H., Penn A. S., Freimuth P. I., Treptow N., Wentzel S., Cleveland W. L., Erlanger B. F. Anti-idiotypic route to anti-acetylcholine receptor antibodies and experimental myasthenia gravis. Proc Natl Acad Sci U S A. 1982 Aug;79(15):4810–4814. doi: 10.1073/pnas.79.15.4810. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wilkin T., Hoskins P. J., Armitage M., Rodier M., Casey C., Diaz J. L., Pyke D. A., Leslie R. D. Value of insulin autoantibodies as serum markers for insulin-dependent diabetes mellitus. Lancet. 1985 Mar 2;1(8427):480–481. doi: 10.1016/s0140-6736(85)92086-0. [DOI] [PubMed] [Google Scholar]
  16. Yale J. F., Marliss E. B. Altered immunity and diabetes in the BB rat. Clin Exp Immunol. 1984 Jul;57(1):1–11. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES