Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1987 Feb;67(2):415–424.

Heterogeneity of non-lymphoid cells expressing HLA-D region antigens in human fetal gut.

J Spencer, T T MacDonald, P G Isaacson
PMCID: PMC1542580  PMID: 3301095

Abstract

Human fetal ileum contains an abundance of cells expressing HLA-D region (HLA-DR) antigens. In this study we characterized the HLA-DR positive cellular infiltrate in fetal ileum using a panel of monoclonal antibodies against cells of the macrophage/monocyte lineage. As well as anti-HLA-DR, the whole infiltrate was recognized by three of the antibodies in the panel studied: RFD1, reported to be an antibody to 'dendritic cells', leu3a which recognizes CD4 (an antigen expressed on helper/inducer T cells and macrophages), and PD7/26 which recognizes the leukocyte common antigen. The macrophage specific antibodies RFD7 and 3.9 stained fewer cells than the anti-HLA-DR. By sequential staining it was clear that most of the RFD7 positive macrophages in the fetal gut lamina propria were not recognized by 3.9 which is a broad specificity antimacrophage antibody in adult tissue. In contrast to this, more of the macrophages within the Peyer's patches of the fetal gut were recognized by 3.9 than by RFD7. In fetal lamina propria not all of the HLA-D region positive cells expressed macrophage markers and some expressed both markers associated with macrophages and 'dendritic' cells. Macrophages with different surface phenotypes were differentially distributed between the lamina propria and the primitive Peyer's patches; RFD7+, 3.9- macrophages were concentrated in the lamina propria whereas RFD7-, 3.9+ macrophages were abundant in Peyer's patches. Within the Peyer's patch lymphoid tissue RFD7+, 3.9- cells were present in the T cell zone whereas RFD7-, 3.9+ cells were concentrated in the dome region as they are in the adult. This suggests that functional heterogeneity of organized macrophage populations may occur in fetal ileum which is free of dietary and bacterial antigens.

Full text

PDF
415

Images in this article

417Fig. 1
on p.417
418Fig. 1 (b)
on p.418
419Fig. 1 (c)
on p.419
420Fig. 2
on p.420
421Fig. 2 (b)
on p.421
422Fig. 3
on p.422
423Fig. 3 (b)
on p.423

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bodmer J. G., Bodmer W. F. Monoclonal antibodies to HLA determinants. Br Med Bull. 1984 Jul;40(3):267–275. doi: 10.1093/oxfordjournals.bmb.a071988. [DOI] [PubMed] [Google Scholar]
  2. Hogg N., Ross G. D., Jones D. B., Slusarenko M., Walport M. J., Lachmann P. J. Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1). Eur J Immunol. 1984 Mar;14(3):236–243. doi: 10.1002/eji.1830140307. [DOI] [PubMed] [Google Scholar]
  3. Hogg N., Takacs L., Palmer D. G., Selvendran Y., Allen C. The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules. Eur J Immunol. 1986 Mar;16(3):240–248. doi: 10.1002/eji.1830160306. [DOI] [PubMed] [Google Scholar]
  4. Janossy G., Bofill M., Poulter L. W., Rawlings E., Burford G. D., Navarrete C., Ziegler A., Kelemen E. Separate ontogeny of two macrophage-like accessory cell populations in the human fetus. J Immunol. 1986 Jun 15;136(12):4354–4361. [PubMed] [Google Scholar]
  5. Mayrhofer G., Pugh C. W., Barclay A. N. The distribution, ontogeny and origin in the rat of Ia-positive cells with dendritic morphology and of Ia antigen in epithelia, with special reference to the intestine. Eur J Immunol. 1983 Feb;13(2):112–122. doi: 10.1002/eji.1830130206. [DOI] [PubMed] [Google Scholar]
  6. Poulter L. W., Campbell D. A., Munro C., Janossy G. Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies. Scand J Immunol. 1986 Sep;24(3):351–357. doi: 10.1111/j.1365-3083.1986.tb02104.x. [DOI] [PubMed] [Google Scholar]
  7. Radzun H. J., Parwaresch M. R., Feller A. C., Hansmann M. L. Monocyte/macrophage-specific monoclonal antibody Ki-M1 recognizes interdigitating reticulum cells. Am J Pathol. 1984 Dec;117(3):441–450. [PMC free article] [PubMed] [Google Scholar]
  8. Selby W. S., Poulter L. W., Hobbs S., Jewell D. P., Janossy G. Heterogeneity of HLA-DR-positive histiocytes in human intestinal lamina propria: a combined histochemical and immunohistological analysis. J Clin Pathol. 1983 Apr;36(4):379–384. doi: 10.1136/jcp.36.4.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Spencer J., Finn T., Isaacson P. G. Expression of HLA-DR antigens on epithelium associated with lymphoid tissue in the human gastrointestinal tract. Gut. 1986 Feb;27(2):153–157. doi: 10.1136/gut.27.2.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Todd R. F., 3rd, Nadler L. M., Schlossman S. F. Antigens on human monocytes identified by monoclonal antibodies. J Immunol. 1981 Apr;126(4):1435–1442. [PubMed] [Google Scholar]
  11. Watson A. J., DeMars R., Trowbridge I. S., Bach F. H. Detection of a novel human class II HLA antigen. 1983 Jul 28-Aug 3Nature. 304(5924):358–361. doi: 10.1038/304358a0. [DOI] [PubMed] [Google Scholar]
  12. Wilders M. M., Sminia T., Janse E. M. Ontogeny of non-lymphoid and lymphoid cells in the rat gut with special reference to large mononuclear Ia-positive dendritic cells. Immunology. 1983 Oct;50(2):303–314. [PMC free article] [PubMed] [Google Scholar]
  13. Wood G. S., Warner N. L., Warnke R. A. Anti-Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage. J Immunol. 1983 Jul;131(1):212–216. [PubMed] [Google Scholar]
  14. van Heyningen V., Guy K., Newman R., Steel C. M. Human MHC class II molecules as differentiation markers. Immunogenetics. 1982;16(5):459–469. doi: 10.1007/BF00372104. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES