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. 1987 Feb;67(2):398–405.

Antigen presentation by a subset of T8+ Ia+ cells to T4+ helper cells.

R Brines, T Lehner
PMCID: PMC1542583  PMID: 2955972

Abstract

The helper function of human T4+ cells acting on autologous peripheral blood B cells was elicited by the hapten-carrier conjugate DNP-streptococcal antigen (DNP-SA), in the presence of monocyte-enriched cells. The antigen presenting function of monocyte-enriched cells can be replaced by a subset of autologous T8+ Vicia villosa lectin adherent (T8VV+) cells. Cell depletion studies confirmed that the T8VV+ cell presentation was mediated by a T cell since killing with anti-T3 antibody and complement removed the antigen presenting capacity of T8VV+ cells but not of monocyte-enriched cells. Furthermore, killing with anti-Ia specific monoclonal antibody and complement abrogates antigen presentation by the T8VV+ cells, suggesting that the latter express an Ia gene product. The antigen presentation is specific to DNP-SA which elicits anti-DNP IgM antibodies, as the latter are not produced in response to tetanus toxoid. We suggest that interactions between T cells may occur not only by T4+ cells inducing T8+ suppressor cell functions but also the reverse activity of T8+ cell presenting antigen to T4+ cells to induce helper function in the absence of other accessory cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burger D. R., Ford D., Vetto R. M., Hamblin A., Goldstein A., Hubbard M., Dumonde D. C. Endothelial cell presentation of antigen to human T cells. Hum Immunol. 1981 Nov;3(3):209–230. doi: 10.1016/0198-8859(81)90019-7. [DOI] [PubMed] [Google Scholar]
  2. Engleman E. G., Benike C. J., Grumet F. C., Evans R. L. Activation of human T lymphocyte subsets: helper and suppressor/cytotoxic T cells recognize and respond to distinct histocompatibility antigens. J Immunol. 1981 Nov;127(5):2124–2129. [PubMed] [Google Scholar]
  3. Lanzavecchia A. Antigen-specific interaction between T and B cells. Nature. 1985 Apr 11;314(6011):537–539. doi: 10.1038/314537a0. [DOI] [PubMed] [Google Scholar]
  4. Lehner T. Antigen-binding human T suppressor cells and their association with the HLA-DR locus. Eur J Immunol. 1983 May;13(5):370–378. doi: 10.1002/eji.1830130505. [DOI] [PubMed] [Google Scholar]
  5. Lehner T., Avery J., Jones T. Separation and characterization of a subset of human T8+ cells which function as antigen-presenting and contrasuppressor cells. Immunology. 1985 Apr;54(4):713–722. [PMC free article] [PubMed] [Google Scholar]
  6. Lehner T., Jones T. The role of MHC class II antigenic determinants in the function of human antigen binding T8+ cells, monocytes and helper and suppressor factors. Clin Exp Immunol. 1984 Jun;56(3):683–693. [PMC free article] [PubMed] [Google Scholar]
  7. Mogensen C. E. The glomerular permeability determined by dextran clearance using Sephadex gel filtration. Scand J Clin Lab Invest. 1968;21(1):77–82. doi: 10.3109/00365516809076979. [DOI] [PubMed] [Google Scholar]
  8. Morimoto C., Reinherz E. L., Schlossman S. F. Regulation of in vitro primary anti-DNP antibody production by functional subsets of T lymphocytes in man. J Immunol. 1981 Jul;127(1):69–73. [PubMed] [Google Scholar]
  9. Nadler P. I., Klingenstein R. J., Richman L. K., Ahmann G. B. The murine Kupffer cell. II. Accessory cell function in in vitro primary antibody responses, mitogen-induced proliferation, and stimulation of mixed lymphocyte responses. J Immunol. 1980 Dec;125(6):2521–2525. [PubMed] [Google Scholar]
  10. Pellegrino M. A., Ferrone S., Theofilopoulos A. N. Isolation of human T and B lymphocytes by rosette formation with 2-aminoethylisothiquronium bromide (AET) -treated sheep red blood cells with monkey red blood cells. J Immunol Methods. 1976;11(3-4):273–279. doi: 10.1016/0022-1759(76)90120-4. [DOI] [PubMed] [Google Scholar]
  11. Rosenwasser L. J., Rosenthal A. S. Adherent cell function in murine T lymphocyte antigen recognition. I. A. macrophage-dependent T cell proliferation assay in the mouse. J Immunol. 1978 Jun;120(6):1991–1995. [PubMed] [Google Scholar]
  12. Russell M. W., Bergmeier L. A., Zanders E. D., Lehner T. Protein antigens of Streptococcus mutans: purification and properties of a double antigen and its protease-resistant component. Infect Immun. 1980 May;28(2):486–493. doi: 10.1128/iai.28.2.486-493.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sopori M. L., Cohen D. A., Cherian S., Kaplan A. M. Antigen presentation in the rat: role of a nonadherent, nonphagocytic, W3/13, OX-6 positive T cell in the presentation of antigen to primed T lymphocytes. Cell Immunol. 1984 Aug;87(1):177–191. doi: 10.1016/0008-8749(84)90142-4. [DOI] [PubMed] [Google Scholar]
  14. Stingl G., Gazze-Stingl L. A., Aberer W., Wolff K. Antigen presentation by murine epidermal langerhans cells and its alteration by ultraviolet B light. J Immunol. 1981 Oct;127(4):1707–1713. [PubMed] [Google Scholar]
  15. Sunshine G. H., Katz D. R., Feldmann M. Dendritic cells induce T cell proliferation to synthetic antigens under Ir gene control. J Exp Med. 1980 Dec 1;152(6):1817–1822. doi: 10.1084/jem.152.6.1817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wysocki L. J., Sato V. L. "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844–2848. doi: 10.1073/pnas.75.6.2844. [DOI] [PMC free article] [PubMed] [Google Scholar]

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