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. 1987 Feb;67(2):383–390.

Immunohistological evidence of lymphokine production and lymphocyte activation antigens in tuberculin reactions.

M A Fullmer, J Y Shen, R L Modlin, T H Rea
PMCID: PMC1542600  PMID: 3038437

Abstract

Evidence of lymphokine elaboration and lymphocyte activation was sought in tuberculin skin test reactions at 24 and 48, or 48 and 96 h in patients with active, culture-proven, pulmonary tuberculosis. Through the use of frozen sections, immunoperoxidase techniques and monoclonal antibodies, anti-interleukin 2 positive cells were found to constitute 0.4% to 0.6% of the dermal infiltrate, and keratinocyte Ia expression at 96 h was consistent with a marker for interferon-gamma production. Cells bearing the interleukin 2 receptor more than doubled in prevalence from 24 to 48 or 96 h but cells staining with Ta1, an antibody identifying activated lymphocytes, were 10% of the cells of the infiltrate at all three times. One-half of the cells of the infiltrate were OKM1-positive, presumably macrophages, perhaps reflecting the presence of active tuberculosis.

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Selected References

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