Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1987 Feb;67(2):252–261.

Immunity to schistosomiasis mansoni in guinea-pigs vaccinated with radiation-attenuated cercariae: I. Humoral responses against skin-stage schistosomula.

J R Gordon, D J McLaren
PMCID: PMC1542601  PMID: 3111760

Abstract

The anti-schistosomular humoral responses of guinea-pigs vaccinated with radiation-attenuated cercariae of Schistosoma mansoni have been investigated in vitro. The sera of vaccinated animals contain schistosomulicidal complement-fixing antibodies which peak in titre at week 5 after vaccination and predominantly consist of IgG2 and IgM antibodies. The ability of the serum to arm macrophages from normal animals to bind to schistosomula, also peaks in titre at week 5 and is associated with IgG2 antibodies. Basophils from normal animals can be sensitized in vitro by vaccine serum to degranulate in the presence of schistosomular antigens. This anaphylactic antibody activity is associated with IgG1 but not IgE antibodies, and peaks in titre at week 10. Three antigens (14 kD, 20 kD and 43 kD) are specifically and transiently detected by vaccine serum on Western blots of schistosomular proteins; these antigens are first discernible at week 4, but were virtually undetectable at week 12.

Full text

PDF
252

Images in this article

258Fig. 6
on p.258

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Capron A., Dessaint J. P., Capron M., Joseph M., Pestel J. Role of anaphylactic antibodies in immunity to schistosomes. Am J Trop Med Hyg. 1980 Sep;29(5):849–857. doi: 10.4269/ajtmh.1980.29.849. [DOI] [PubMed] [Google Scholar]
  2. Correa-Oliveira R., Sher A. Defective immunoglobulin M responses to vaccination or infection with Schistosoma mansoni in xid mice. Infect Immun. 1985 Nov;50(2):409–414. doi: 10.1128/iai.50.2.409-414.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coupland K., Leslie R. G. The expression of Fc receptors on guinea-pig peritoneal macrophages and neutrophils. Immunology. 1983 Apr;48(4):647–656. [PMC free article] [PubMed] [Google Scholar]
  4. Dean D. A., Wistar R., Chen P. Immune response of guinea pigs to Schistosoma mansoni. I. In vitro effects of antibody and neutrophils, eosinophils and macrophages on schistosomula. Am J Trop Med Hyg. 1975 Jan;24(1):74–82. doi: 10.4269/ajtmh.1975.24.74. [DOI] [PubMed] [Google Scholar]
  5. Haynes J. D., Rosenstein R. W., Askenase P. W. A newly described activity of guinea pig IgG1 antibodies: transfer of cutaneous basophil reactions. J Immunol. 1978 Mar;120(3):886–894. [PubMed] [Google Scholar]
  6. James S. L., Correa-Oliveira R., Leonard E. J. Defective vaccine-induced immunity to Schistosoma mansoni in P strain mice. II. Analysis of cellular responses. J Immunol. 1984 Sep;133(3):1587–1593. [PubMed] [Google Scholar]
  7. James S. L., Natovitz P. C., Farrar W. L., Leonard E. J. Macrophages as effector cells of protective immunity in murine schistosomiasis: macrophage activation in mice vaccinated with radiation-attenuated cercariae. Infect Immun. 1984 Jun;44(3):569–575. doi: 10.1128/iai.44.3.569-575.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. James S. L., Sher A., Lazdins J. K., Meltzer M. S. Macrophages as effector cells of protective immunity in murine schistosomiasis. II. Killing of newly transformed schistosomula in vitro by macrophages activated as a consequence of Schistosoma mansoni infection. J Immunol. 1982 Apr;128(4):1535–1540. [PubMed] [Google Scholar]
  9. Joseph M., Auriault C., Capron A., Vorng H., Viens P. A new function for platelets: IgE-dependent killing of schistosomes. Nature. 1983 Jun 30;303(5920):810–812. doi: 10.1038/303810a0. [DOI] [PubMed] [Google Scholar]
  10. Kauffman H. F., Levering P. R., De Vries K. A single centrifugation step method for the simultaneous separation of different leukocytes with special reference to basophilic leukocytes. J Immunol Methods. 1983 Feb 25;57(1-3):1–7. doi: 10.1016/0022-1759(83)90058-3. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Lanar D. E., Pearce E. J., Sher A. Expression in Escherichia coli of two Schistosoma mansoni genes that encode major antigens recognized by immune mice. Mol Biochem Parasitol. 1985 Oct;17(1):45–60. doi: 10.1016/0166-6851(85)90127-6. [DOI] [PubMed] [Google Scholar]
  14. McLaren D. J., Pearce E. J., Smithers S. R. Site potential for challenge attrition in mice, rats and guinea pigs vaccinated with irradiated cercariae of Schistosoma mansoni. Parasite Immunol. 1985 Jan;7(1):29–44. doi: 10.1111/j.1365-3024.1985.tb00477.x. [DOI] [PubMed] [Google Scholar]
  15. Minard P., Dean D. A., Jacobson R. H., Vannier W. E., Murrell K. D. Immunization of mice with cobalt-60 irradiated Schistosoma mansoni cercariae. Am J Trop Med Hyg. 1978 Jan;27(1 Pt 1):76–86. doi: 10.4269/ajtmh.1978.27.76. [DOI] [PubMed] [Google Scholar]
  16. Moqbel R., MacDonald A. J., Kay A. B. Enhancement of human eosinophil- and neutrophil-mediated killing of schistosomula of Schistosoma mansoni by reversed type (IgE-mediated) anaphylaxis, in vitro. Clin Exp Immunol. 1985 Mar;59(3):577–586. [PMC free article] [PubMed] [Google Scholar]
  18. Payares G., McLaren D. J., Evans W. H., Smithers S. R. Changes in the surface antigen profile of Schistosoma mansoni during maturation from cercaria to adult worm. Parasitology. 1985 Aug;91(Pt 1):83–99. doi: 10.1017/s0031182000056535. [DOI] [PubMed] [Google Scholar]
  19. Pearce E. J., McLaren D. J. Reappraisal of the guinea-pig as an experimental host for studies of schistosomiasis mansoni. Parasitology. 1983 Dec;87(Pt 3):455–464. doi: 10.1017/s0031182000082986. [DOI] [PubMed] [Google Scholar]
  20. Pearce E. J., McLaren D. J. Schistosoma mansoni: in vivo and in vitro studies of immunity using the guinea-pig model. Parasitology. 1983 Dec;87(Pt 3):465–479. doi: 10.1017/s0031182000082998. [DOI] [PubMed] [Google Scholar]
  21. Pierce R. J., Aimar C., Balloul J. M., Delarue M., Grausz D., Verwaerde C., Capron A. Translation of Schistosoma mansoni antigens in Xenopus oocytes microinjected with mRNA from adult worms. Mol Biochem Parasitol. 1985 May;15(2):171–188. doi: 10.1016/0166-6851(85)90118-5. [DOI] [PubMed] [Google Scholar]
  22. Ramalho-Pinto F. J., Gazzinelli G., Howells R. E., Mota-Santos T. A., Figueiredo E. A., Pellegrino J. Schistosoma mansoni: defined system for stepwise transformation of cercaria to schistosomule in vitro. Exp Parasitol. 1974 Dec;36(3):360–372. doi: 10.1016/0014-4894(74)90076-9. [DOI] [PubMed] [Google Scholar]
  23. Ramalho-Pinto F. J., McLaren D. J., Smithers S. R. Complement-mediated killing of schistosomula of Schistosoma mansoni by rat eosinophils in vitro. J Exp Med. 1978 Jan 1;147(1):147–156. doi: 10.1084/jem.147.1.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sher A., Correa-Oliveira R., Hieny S., Hussain R. Mechanisms of protective immunity against Schistosoma mansoni infection in mice vaccinated with irradiated cercariae. IV. Analysis of the role of IgE antibodies and mast cells. J Immunol. 1983 Sep;131(3):1460–1465. [PubMed] [Google Scholar]
  25. Sher A., Hieny S., James S. L., Asofsky R. Mechanisms of protective immunity against Schistosoma mansoni infection in mice vaccinated with irradiated cercariae. II. Analysis of immunity in hosts deficient in T lymphocytes, B lymphocytes, or complement. J Immunol. 1982 Apr;128(4):1880–1884. [PubMed] [Google Scholar]
  26. Simpson A. J., Hackett F., Walker T., de Rossi R., Smithers S. R. Antibody response against schistosomulum surface antigens and protective immunity following immunization with highly irradiated cercariae of Schistosoma mansoni. Parasite Immunol. 1985 Mar;7(2):133–152. doi: 10.1111/j.1365-3024.1985.tb00065.x. [DOI] [PubMed] [Google Scholar]
  27. Spiegelberg H. L. Biological activities of immunoglobulins of different classes and subclasses. Adv Immunol. 1974;19(0):259–294. doi: 10.1016/s0065-2776(08)60254-0. [DOI] [PubMed] [Google Scholar]
  28. Verwaerde C., Auriault C., Damonneville M., Grzych J. M., Pierce R., Capron A. IgG response of rats and humans to the released products of schistosomula of Schistosoma mansoni. Parasitology. 1985 Jun;90(Pt 3):509–518. doi: 10.1017/s0031182000055505. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES