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. 1987 Feb;67(2):352–361.

Heterogeneity of human anti-MAG IgM as revealed by their reactivity on avian embryonic tissues.

G C Tucker, K Dellagi, C Schmitt, J C Brouet, J P Thiery
PMCID: PMC1542607  PMID: 2440627

Abstract

Monoclonal IgM from patients with peripheral neuropathies frequently have an antibody activity directed against myelin-associated glycoprotein (MAG). Reactivity of 19 of these sera with avian embryonic tissues was compared. Immunohistological studies allowed their classification into distinct groups according to the staining pattern. Both neural and non-neural cell lineages were recognized by these antibodies. The IgM specificities were also compared with those of the monoclonal antibodies HNK-1 and NC-1, which react with MAG and some cell adhesion molecules. Only one group displayed a pattern of staining similar to that observed with these monoclonals. The reactivity of these IgM paraproteins with two cell adhesion molecules, neural cell adhesion molecule and neuron-glia-cell adhesion molecule by immunoblotting confirmed the heterogeneity found in the histological study.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abo T., Balch C. M. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol. 1981 Sep;127(3):1024–1029. [PubMed] [Google Scholar]
  2. Braun P. E., Frail D. E., Latov N. Myelin-associated glycoprotein is the antigen for a monoclonal IgM in polyneuropathy. J Neurochem. 1982 Nov;39(5):1261–1265. doi: 10.1111/j.1471-4159.1982.tb12563.x. [DOI] [PubMed] [Google Scholar]
  3. Chou K. H., Ilyas A. A., Evans J. E., Quarles R. H., Jungalwala F. B. Structure of a glycolipid reacting with monoclonal IgM in neuropathy and with HNK-1. Biochem Biophys Res Commun. 1985 Apr 16;128(1):383–388. doi: 10.1016/0006-291x(85)91690-0. [DOI] [PubMed] [Google Scholar]
  4. Crossin K. L., Hoffman S., Grumet M., Thiery J. P., Edelman G. M. Site-restricted expression of cytotactin during development of the chicken embryo. J Cell Biol. 1986 May;102(5):1917–1930. doi: 10.1083/jcb.102.5.1917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dellagi K., Brouet J. C. Antigen shared between myelin and NK cells. Lancet. 1984 Jun 2;1(8388):1244–1244. doi: 10.1016/s0140-6736(84)91732-x. [DOI] [PubMed] [Google Scholar]
  6. Dellagi K., Dupouey P., Brouet J. C., Billecocq A., Gomez D., Clauvel J. P., Seligmann M. Waldenström's macroglobulinemia and peripheral neuropathy: a clinical and immunologic study of 25 patients. Blood. 1983 Aug;62(2):280–285. [PubMed] [Google Scholar]
  7. Edelman G. M. Cell adhesion and the molecular processes of morphogenesis. Annu Rev Biochem. 1985;54:135–169. doi: 10.1146/annurev.bi.54.070185.001031. [DOI] [PubMed] [Google Scholar]
  8. Edelman G. M. Modulation of cell adhesion during induction, histogenesis, and perinatal development of the nervous system. Annu Rev Neurosci. 1984;7:339–377. doi: 10.1146/annurev.ne.07.030184.002011. [DOI] [PubMed] [Google Scholar]
  9. Frail D. E., Edwards A. M., Braun P. E. Molecular characteristics of the epitope in myelin-associated glycoprotein that is recognized by a monoclonal IgM in human neuropathy patients. Mol Immunol. 1984 Aug;21(8):721–725. doi: 10.1016/0161-5890(84)90024-5. [DOI] [PubMed] [Google Scholar]
  10. Fraser S. E., Murray B. A., Chuong C. M., Edelman G. M. Alteration of the retinotectal map in Xenopus by antibodies to neural cell adhesion molecules. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4222–4226. doi: 10.1073/pnas.81.13.4222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grumet M., Edelman G. M. Heterotypic binding between neuronal membrane vesicles and glial cells is mediated by a specific cell adhesion molecule. J Cell Biol. 1984 May;98(5):1746–1756. doi: 10.1083/jcb.98.5.1746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Grumet M., Hoffman S., Edelman G. M. Two antigenically related neuronal cell adhesion molecules of different specificities mediate neuron-neuron and neuron-glia adhesion. Proc Natl Acad Sci U S A. 1984 Jan;81(1):267–271. doi: 10.1073/pnas.81.1.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gulcher J. R., Marton L. S., Stefansson K. Two large glycosylated polypeptides found in myelinating oligodendrocytes but not in myelin. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2118–2122. doi: 10.1073/pnas.83.7.2118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hoffman S., Chuong C. M., Edelman G. M. Evolutionary conservation of key structures and binding functions of neural cell adhesion molecules. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6881–6885. doi: 10.1073/pnas.81.21.6881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hoffman S., Sorkin B. C., White P. C., Brackenbury R., Mailhammer R., Rutishauser U., Cunningham B. A., Edelman G. M. Chemical characterization of a neural cell adhesion molecule purified from embryonic brain membranes. J Biol Chem. 1982 Jul 10;257(13):7720–7729. [PubMed] [Google Scholar]
  16. Ilyas A. A., Quarles R. H., Brady R. O. The monoclonal antibody HNK-1 reacts with a human peripheral nerve ganglioside. Biochem Biophys Res Commun. 1984 Aug 16;122(3):1206–1211. doi: 10.1016/0006-291x(84)91220-8. [DOI] [PubMed] [Google Scholar]
  17. Ilyas A. A., Quarles R. H., Dalakas M. C., Brady R. O. Polyneuropathy with monoclonal gammopathy: glycolipids are frequently antigens for IgM paraproteins. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6697–6700. doi: 10.1073/pnas.82.19.6697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ilyas A. A., Quarles R. H., MacIntosh T. D., Dobersen M. J., Trapp B. D., Dalakas M. C., Brady R. O. IgM in a human neuropathy related to paraproteinemia binds to a carbohydrate determinant in the myelin-associated glycoprotein and to a ganglioside. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1225–1229. doi: 10.1073/pnas.81.4.1225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kelly J. J., Jr, Kyle R. A., O'Brien P. C., Dyck P. J. Prevalence of monoclonal protein in peripheral neuropathy. Neurology. 1981 Nov;31(11):1480–1483. doi: 10.1212/wnl.31.11.1480. [DOI] [PubMed] [Google Scholar]
  20. Kruse J., Keilhauer G., Faissner A., Timpl R., Schachner M. The J1 glycoprotein--a novel nervous system cell adhesion molecule of the L2/HNK-1 family. Nature. 1985 Jul 11;316(6024):146–148. doi: 10.1038/316146a0. [DOI] [PubMed] [Google Scholar]
  21. Kruse J., Mailhammer R., Wernecke H., Faissner A., Sommer I., Goridis C., Schachner M. Neural cell adhesion molecules and myelin-associated glycoprotein share a common carbohydrate moiety recognized by monoclonal antibodies L2 and HNK-1. Nature. 1984 Sep 13;311(5982):153–155. doi: 10.1038/311153a0. [DOI] [PubMed] [Google Scholar]
  22. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  23. Latov N., Braun P. E., Gross R. B., Sherman W. H., Penn A. S., Chess L. Plasma cell dyscrasia and peripheral neuropathy: identification of the myelin antigens that react with human paraproteins. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7139–7142. doi: 10.1073/pnas.78.11.7139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Latov N., Sherman W. H., Nemni R., Galassi G., Shyong J. S., Penn A. S., Chess L., Olarte M. R., Rowland L. P., Osserman E. F. Plasma-cell dyscrasia and peripheral neuropathy with a monoclonal antibody to peripheral-nerve myelin. N Engl J Med. 1980 Sep 11;303(11):618–621. doi: 10.1056/NEJM198009113031105. [DOI] [PubMed] [Google Scholar]
  25. Marton L. S., Stefansson K. Developmental alterations in molecular weights of proteins in the human central nervous system that react with antibodies against myelin-associated glycoprotein. J Cell Biol. 1984 Nov;99(5):1642–1646. doi: 10.1083/jcb.99.5.1642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. McClain D. A., Edelman G. M. A neural cell adhesion molecule from human brain. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6380–6384. doi: 10.1073/pnas.79.20.6380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. McGarry R. C., Helfand S. L., Quarles R. H., Roder J. C. Recognition of myelin-associated glycoprotein by the monoclonal antibody HNK-1. Nature. 1983 Nov 24;306(5941):376–378. doi: 10.1038/306376a0. [DOI] [PubMed] [Google Scholar]
  28. Melmed C., Frail D., Duncan I., Braun P., Danoff D., Finlayson M., Stewart J. Peripheral neuropathy with IgM kappa monoclonal immunoglobulin directed against myelin-associated glycoprotein. Neurology. 1983 Nov;33(11):1397–1405. doi: 10.1212/wnl.33.11.1397. [DOI] [PubMed] [Google Scholar]
  29. Murray N., Steck A. J. Indication of a possible role in a demyelinating neuropathy for an antigen shared between myelin and NK cells. Lancet. 1984 Mar 31;1(8379):711–713. doi: 10.1016/s0140-6736(84)92224-4. [DOI] [PubMed] [Google Scholar]
  30. Quarles R. H., Everly J. L., Brady R. O. Demonstration of a glycoprotein which is associated with a purified myelin fraction from rat brain. Biochem Biophys Res Commun. 1972 Apr 28;47(2):491–497. doi: 10.1016/0006-291x(72)90741-3. [DOI] [PubMed] [Google Scholar]
  31. Quarles R. H., Everly J. L., Brady R. O. Myelin-associated glycoprotein: a developmental change. Brain Res. 1973 Aug 30;58(2):506–509. doi: 10.1016/0006-8993(73)90022-x. [DOI] [PubMed] [Google Scholar]
  32. Quarles R. H., Pasnak C. F. A rapid procedure for selectively isolating the major glycoprotein from purified rat brain myelin. Biochem J. 1977 Jun 1;163(3):635–637. doi: 10.1042/bj1630635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Schachner M., Faissner A., Kruse J., Lindner J., Meier D. H., Rathjen F. G., Wernecke H. Cell-type specificity and developmental expression of neural cell-surface components involved in cell interactions and of structurally related molecules. Cold Spring Harb Symp Quant Biol. 1983;48(Pt 2):557–568. doi: 10.1101/sqb.1983.048.01.060. [DOI] [PubMed] [Google Scholar]
  34. Schuller-Petrovic S., Gebhart W., Lassmann H., Rumpold H., Kraft D. A shared antigenic determinant between natural killer cells and nervous tissue. Nature. 1983 Nov 10;306(5939):179–181. doi: 10.1038/306179a0. [DOI] [PubMed] [Google Scholar]
  35. Shy M. E., Vietorisz T., Nobile-Orazio E., Latov N. Specificity of human IgM M-proteins that bind to myelin-associated glycoprotein: peptide mapping, deglycosylation, and competitive binding studies. J Immunol. 1984 Nov;133(5):2509–2512. [PubMed] [Google Scholar]
  36. Steck A. J., Murray N., Vandevelde M., Zurbriggen A. Human monoclonal antibodies to myelin-associated glycoprotein. Comparison of specificity and use for immunocytochemical localisation of the antigen. J Neuroimmunol. 1983 Oct;5(2):145–156. doi: 10.1016/0165-5728(83)90005-x. [DOI] [PubMed] [Google Scholar]
  37. Thiery J. P., Duband J. L., Rutishauser U., Edelman G. M. Cell adhesion molecules in early chicken embryogenesis. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6737–6741. doi: 10.1073/pnas.79.21.6737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Thiery J. P., Duband J. L., Tucker G. C. Cell migration in the vertebrate embryo: role of cell adhesion and tissue environment in pattern formation. Annu Rev Cell Biol. 1985;1:91–113. doi: 10.1146/annurev.cb.01.110185.000515. [DOI] [PubMed] [Google Scholar]
  39. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Tucker G. C., Aoyama H., Lipinski M., Tursz T., Thiery J. P. Identical reactivity of monoclonal antibodies HNK-1 and NC-1: conservation in vertebrates on cells derived from the neural primordium and on some leukocytes. Cell Differ. 1984 Aug;14(3):223–230. doi: 10.1016/0045-6039(84)90049-6. [DOI] [PubMed] [Google Scholar]
  41. Vincent M., Duband J. L., Thiery J. P. A cell surface determinant expressed early on migrating avian neural crest cells. Brain Res. 1983 Aug;285(2):235–238. doi: 10.1016/0165-3806(83)90058-5. [DOI] [PubMed] [Google Scholar]
  42. Wood E., Feizi T. Blood group I and i activities of straight chain and branched synthetic oligosaccharides related to the precursors of the major blood group antigens. FEBS Lett. 1979 Aug 1;104(1):135–140. doi: 10.1016/0014-5793(79)81100-x. [DOI] [PubMed] [Google Scholar]

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