Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1987 Feb;67(2):454–459.

Sperm-egg penetration of human spermatozoa treated with various rabbit antisera to human sperm antigens.

T Samuel, Y Soffer, E Caspi
PMCID: PMC1542608  PMID: 3649279

Abstract

The effect of rabbit antisera to human whole spermatozoa, to human lactate dehydrogenase (LDH-X) and to human protamine 1 (HP1) and protamine 2 (HP2), was tested in the hamster zona-free egg penetration assay with human spermatozoa. The human spermatozoa were incubated with TEST yolk buffer washed and incubated with the antisera before penetration assay. Only antiserum to whole spermatozoa could inhibit the penetration capacity of treated human spermatozoa. The significance of the results as well as the advantage of the method used by us are discussed.

Full text

PDF
454

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander N. J. Antibodies to human spermatozoa impede sperm penetration of cervical mucus or hamster eggs. Fertil Steril. 1984 Mar;41(3):433–439. [PubMed] [Google Scholar]
  2. Barros C., Gonzalez J., Herrera E., Bustos-Obregon E. Human sperm penetration into zona-free hamster oocytes as a test to evaluate the sperm fertilizing ability. Andrologia. 1979 May-Jun;11(3):197–210. doi: 10.1111/j.1439-0272.1979.tb02186.x. [DOI] [PubMed] [Google Scholar]
  3. Barros C., Gonzélez J., Herrera E., Bustos-Obregón E. Fertilizing capacity of human spermatozoa evaluated by actual penetration of foreign eggs. Contraception. 1978 Jan;17(1):87–92. doi: 10.1016/0010-7824(78)90064-1. [DOI] [PubMed] [Google Scholar]
  4. Binor Z., Sokoloski J. E., Wolf D. P. Penetration of the zona-free hamster egg by human sperm. Fertil Steril. 1980 Mar;33(3):321–327. doi: 10.1016/s0015-0282(16)44602-9. [DOI] [PubMed] [Google Scholar]
  5. Cohen J., Weber R. F., van der Vijver J. C., Zeilmaker G. H. In vitro fertilizing capacity of human spermatozoa with the use of zona-free hamster ova: interassay variation and prognostic value. Fertil Steril. 1982 Apr;37(4):565–572. doi: 10.1016/s0015-0282(16)46167-4. [DOI] [PubMed] [Google Scholar]
  6. Goldberg E., Wheat T. E., Powell J. E., Stevens V. C. Reduction of fertility in female baboons immunized with lactate dehydrogenase C4. Fertil Steril. 1981 Feb;35(2):214–217. doi: 10.1016/s0015-0282(16)45325-2. [DOI] [PubMed] [Google Scholar]
  7. Jaskey D. G., Cohen M. R. Twenty-four to ninety-six-hour storage of human spermatozoa in test-yolk buffer. Fertil Steril. 1981 Feb;35(2):205–208. doi: 10.1016/s0015-0282(16)45323-9. [DOI] [PubMed] [Google Scholar]
  8. Johnson J. P., Alexander N. J. Hamster egg penetration: comparison of preincubation periods. Fertil Steril. 1984 Apr;41(4):599–602. [PubMed] [Google Scholar]
  9. Kolk A. H., Samuel T. Isolation, chemical and immunological characterization of two strongly basic nuclear proteins from human spermatozoa. Biochim Biophys Acta. 1975 Jun 26;393(2):307–319. doi: 10.1016/0005-2795(75)90057-4. [DOI] [PubMed] [Google Scholar]
  10. Menge A. C., Black C. S. Effects of antisera on human sperm penetration of zona-free hamster ova. Fertil Steril. 1979 Aug;32(2):214–221. doi: 10.1016/s0015-0282(16)44184-1. [DOI] [PubMed] [Google Scholar]
  11. Morton D. B., McAnulty P. A. The effect on fertility of immunizing female sheep with ram sperm acrosin and hyaluronidase. J Reprod Immunol. 1979 Jan-Feb;1(1):61–73. doi: 10.1016/0165-0378(79)90030-5. [DOI] [PubMed] [Google Scholar]
  12. Naz R. K., Saxe J. M., Menge A. C. Inhibition of fertility in rabbits by monoclonal antibodies against sperm. Biol Reprod. 1983 Feb;28(1):249–254. doi: 10.1095/biolreprod28.1.249. [DOI] [PubMed] [Google Scholar]
  13. O'Rand M. G. Inhibition of fertility and sperm-zona binding by antiserum to the rabbit sperm membrane autoantigen RSA-1. Biol Reprod. 1981 Oct;25(3):621–628. doi: 10.1095/biolreprod25.3.621. [DOI] [PubMed] [Google Scholar]
  14. Overstreet J. W., Yanagimachi R., Katz D. F., Hayashi K., Hanson F. W. Penetration of human spermatozoa into the human zona pellucida and the zona-free hamster egg: a study of fertile donors and infertile patients. Fertil Steril. 1980 May;33(5):534–542. doi: 10.1016/s0015-0282(16)44720-5. [DOI] [PubMed] [Google Scholar]
  15. Peterson R. N., Robl J. M., Dziuk P. J., Russell L. D. The effects of antisperm plasma membrane antibodies on sperm-egg binding, penetration, and fertilization in the pig. J Exp Zool. 1982 Sep 20;223(1):79–81. doi: 10.1002/jez.1402230113. [DOI] [PubMed] [Google Scholar]
  16. Rogers B. J. The sperm penetration assay: its usefulness reevaluated. Fertil Steril. 1985 Jun;43(6):821–840. doi: 10.1016/s0015-0282(16)48608-5. [DOI] [PubMed] [Google Scholar]
  17. Rogers B. J., Van Campen H., Ueno M., Lambert H., Bronson R., Hale R. Analysis of human spermatozoal fertilizing ability using zona-free ova. Fertil Steril. 1979 Dec;32(6):664–670. doi: 10.1016/s0015-0282(16)44416-x. [DOI] [PubMed] [Google Scholar]
  18. Rümke P., Hekman A. Sterility: an immunologic disorder? Clin Obstet Gynecol. 1977 Sep;20(3):691–703. doi: 10.1097/00003081-197709000-00017. [DOI] [PubMed] [Google Scholar]
  19. Samuel T., Alexander N. J., Rümke P. Reproducibility of the indirect immunofluorescence test on swollen human spermheads. Clin Exp Immunol. 1977 May;28(2):223–225. [PMC free article] [PubMed] [Google Scholar]
  20. Samuel T., Kolk A. H., Rümke P. Studies on the immunogenicity of protamines in humans and experimental animals by means of a micro-complement fixation test. Clin Exp Immunol. 1978 Aug;33(2):252–260. [PMC free article] [PubMed] [Google Scholar]
  21. Samuel T., Linnet L., Rümke P. Post-vasectomy autoimmunity to protamines in relation to the formation of granulomas and sperm agglutinating antibodies. Clin Exp Immunol. 1978 Aug;33(2):261–269. [PMC free article] [PubMed] [Google Scholar]
  22. Soffer Y., Marcus Z. H., Bukovsky I., Caspi E. Immunological factors and post coital test in unexplained infertility. Int J Fertil. 1976;(2):89–95. [PubMed] [Google Scholar]
  23. Syner F. N., Kuras R., Moghissi K. S. Active immunization of female rabbits with purified rabbit acrosin and effect on fertility. Fertil Steril. 1979 Oct;32(4):468–473. [PubMed] [Google Scholar]
  24. Yanagimachi R. Penetration of guinea-pig spermatozoa into hamster eggs in vitro. J Reprod Fertil. 1972 Mar;28(3):477–480. doi: 10.1530/jrf.0.0280477. [DOI] [PubMed] [Google Scholar]
  25. Yanagimachi R., Yanagimachi H., Rogers B. J. The use of zona-free animal ova as a test-system for the assessment of the fertilizing capacity of human spermatozoa. Biol Reprod. 1976 Nov;15(4):471–476. doi: 10.1095/biolreprod15.4.471. [DOI] [PubMed] [Google Scholar]
  26. Zausner-Guelman B., Blasco L., Wolf D. P. Zona-free hamster eggs and human sperm penetration capacity: a comparative study of proven fertile donors and infertility patients. Fertil Steril. 1981 Dec;36(6):771–777. doi: 10.1016/s0015-0282(16)45924-8. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES